دورية أكاديمية
Functional microRNAs and target sites are created by lineage-specific transposition.
| العنوان: | Functional microRNAs and target sites are created by lineage-specific transposition. |
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| المؤلفون: | Spengler RM; Department of Internal Medicine., Oakley CK, Davidson BL |
| المصدر: | Human molecular genetics [Hum Mol Genet] 2014 Apr 01; Vol. 23 (7), pp. 1783-93. Date of Electronic Publication: 2013 Nov 13. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: IRL Press at Oxford University Press Country of Publication: England NLM ID: 9208958 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1460-2083 (Electronic) Linking ISSN: 09646906 NLM ISO Abbreviation: Hum Mol Genet Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Oxford, England ; New York : IRL Press at Oxford University Press, c1992- |
| مواضيع طبية MeSH: | 3' Untranslated Regions/*genetics , Alu Elements/*genetics , DNA Transposable Elements/*genetics , Long Interspersed Nucleotide Elements/*genetics , MicroRNAs/*genetics, Animals ; Binding Sites/genetics ; Cell Line ; Cell Lineage/genetics ; Gene Expression Regulation ; HEK293 Cells ; HeLa Cells ; Humans ; Mice ; MicroRNAs/biosynthesis ; Pan troglodytes |
| مستخلص: | Transposable elements (TEs) account for nearly one-half of the sequence content in the human genome, and de novo germline transposition into regulatory or coding sequences of protein-coding genes can cause heritable disorders. TEs are prevalent in and around protein-coding genes, providing an opportunity to impart regulation. Computational studies reveal that microRNA (miRNA) genes and miRNA target sites reside within TE sequences, but there is little experimental evidence supporting a role for TEs in the birth of miRNAs, or as platform for gene regulation by miRNAs. In this work, we validate miRNAs and target sites derived from TE families prevalent in the human genome, including the ancient long interspersed nuclear element 2 (LINE2/L2), mammalian-wide interspersed repeat (MIR) retrotransposons and the primate-specific Alu family. We show that genes with 3' untranslated region (3' UTR) MIR elements are enriched for let-7 targets and that these sites are conserved and responsive to let-7 expression. We also demonstrate that 3' UTR-embedded Alus are a source of miR-24 and miR-122 target sites and that a subset of active genomic Alus provide for de novo target site creation. Finally, we report that although the creation of miRNA genes by Alu elements is relatively uncommon relative to their overall genomic abundance, Alu-derived miR-1285-1 is efficiently processed from its genomic locus and regulates genes with target sites contained within homologous elements. Taken together, our data provide additional evidence for TEs as a source for miRNAs and miRNA target sites, with instances of conservation through the course of mammalian evolution. |
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| معلومات مُعتمدة: | P30 ES005605 United States ES NIEHS NIH HHS |
| المشرفين على المادة: | 0 (3' Untranslated Regions) 0 (DNA Transposable Elements) 0 (MIRN122 microRNA, human) 0 (MIRN1285 microRNA, human) 0 (MIRN24 microRNA, human) 0 (MicroRNAs) 0 (mirnlet7 microRNA, human) |
| تواريخ الأحداث: | Date Created: 20131116 Date Completed: 20141126 Latest Revision: 20211021 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC3943519 |
| DOI: | 10.1093/hmg/ddt569 |
| PMID: | 24234653 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1460-2083 |
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| DOI: | 10.1093/hmg/ddt569 |