دورية أكاديمية
Microbial community and potential functional gene diversity involved in anaerobic hydrocarbon degradation and methanogenesis in an oil sands tailings pond.
| العنوان: | Microbial community and potential functional gene diversity involved in anaerobic hydrocarbon degradation and methanogenesis in an oil sands tailings pond. |
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| المؤلفون: | An D; a Department of Biological Sciences, University of Calgary, Calgary, AB T2N 1N4, Canada., Brown D, Chatterjee I, Dong X, Ramos-Padron E, Wilson S, Bordenave S, Caffrey SM, Gieg LM, Sensen CW, Voordouw G |
| المصدر: | Genome [Genome] 2013 Oct; Vol. 56 (10), pp. 612-8. Date of Electronic Publication: 2013 Jul 29. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Canadian Science Publishing Country of Publication: Canada NLM ID: 8704544 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1480-3321 (Electronic) Linking ISSN: 08312796 NLM ISO Abbreviation: Genome Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2011- : Ottawa, ON : Canadian Science Publishing Original Publication: Ottawa, Canada : National Research Council of Canada, c1987- |
| مواضيع طبية MeSH: | Metagenome*, Archaea/*isolation & purification , Bacteria/*isolation & purification , Hydrocarbons/*metabolism , Methane/*biosynthesis , Oil and Gas Fields/*microbiology , Ponds/*microbiology, Alberta ; Archaea/classification ; Archaea/genetics ; Archaea/metabolism ; Bacteria/classification ; Bacteria/genetics ; Bacteria/metabolism ; Metagenomics ; Phylogeny ; RNA, Ribosomal, 16S/genetics ; Sequence Analysis, DNA |
| مستخلص: | Oil sands tailings ponds harbor large amounts of tailings resulting from surface mining of bitumen and consist of water, sand, clays, residual bitumen, and hydrocarbon diluent. Oxygen ingress in these ponds is limited to the surface layers, causing most hydrocarbon degradation to be catalyzed by anaerobic, methanogenic microbial communities. This causes the evolution of large volumes of methane of up to 10(4) m(3)/day. A pyrosequencing survey of 16S rRNA amplicons from 10 samples obtained from different depths indicated the presence of a wide variety of taxa involved in anaerobic hydrocarbon degradation and methanogenesis, including the phyla Proteobacteria, Euryarchaeota, Firmicutes, Actinobacteria, Chloroflexi, and Bacteroidetes. Metagenomic sequencing of DNA isolated from one of these samples indicated a more diverse community than indicated by the 16S rRNA amplicon survey. Both methods indicated the same major phyla to be present. The metagenomic dataset indicated the presence of genes involved in the three stages of anaerobic aromatic hydrocarbon degradation, including genes for enzymes of the peripheral (upper), the central (lower), and the methanogenesis pathways. Upper pathway genes showed broad phylogenetic affiliation (Proteobacteria, Firmicutes, and Actinobacteria), whereas lower pathway genes were mostly affiliated with the Deltaproteobacteria. Genes for both hydrogenotrophic and acetotrophic methanogenesis were also found. The wide variety of taxa involved in initial hydrocarbon degradation through upper pathways may reflect the variety of residual bitumen and diluent components present in the tailings pond. |
| المشرفين على المادة: | 0 (Hydrocarbons) 0 (RNA, Ribosomal, 16S) OP0UW79H66 (Methane) |
| تواريخ الأحداث: | Date Created: 20131119 Date Completed: 20140630 Latest Revision: 20161020 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1139/gen-2013-0083 |
| PMID: | 24237342 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1480-3321 |
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| DOI: | 10.1139/gen-2013-0083 |