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The structure of TAX1BP1 UBZ1+2 provides insight into target specificity and adaptability.

التفاصيل البيبلوغرافية
العنوان: The structure of TAX1BP1 UBZ1+2 provides insight into target specificity and adaptability.
المؤلفون: Ceregido MA; Departamento de Química Física e Instituto de Biotecnología, Facultad de Ciencias, Universidad de Granada, Granada 18071, Spain; Jean Jeener NMR Centre, Structural Biology Brussels, Vrije Universiteit Brussel, Brussels 1050, Belgium; Molecular Recognition Unit, Department of Structural Biology, VIB, Brussels 1050, Belgium., Spínola Amilibia M; Instituto de Biomedicina de Valencia, Consejo Superior de Investigaciones Cientificas, Valencia 46010, Spain; Departamento de Biología Físico-Química, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Cientificas, Madrid 28040, Spain., Buts L; Jean Jeener NMR Centre, Structural Biology Brussels, Vrije Universiteit Brussel, Brussels 1050, Belgium; Molecular Recognition Unit, Department of Structural Biology, VIB, Brussels 1050, Belgium., Rivera-Torres J; Department of Epidemiology, Atherothrombosis and Imaging, Spanish National Cardiovascular Research Center, Madrid 28029, Spain., Garcia-Pino A; Jean Jeener NMR Centre, Structural Biology Brussels, Vrije Universiteit Brussel, Brussels 1050, Belgium; Molecular Recognition Unit, Department of Structural Biology, VIB, Brussels 1050, Belgium., Bravo J; Instituto de Biomedicina de Valencia, Consejo Superior de Investigaciones Cientificas, Valencia 46010, Spain. Electronic address: jbravo@ibv.csic.es., van Nuland NA; Jean Jeener NMR Centre, Structural Biology Brussels, Vrije Universiteit Brussel, Brussels 1050, Belgium; Molecular Recognition Unit, Department of Structural Biology, VIB, Brussels 1050, Belgium. Electronic address: nvnuland@vub.ac.be.
المصدر: Journal of molecular biology [J Mol Biol] 2014 Feb 06; Vol. 426 (3), pp. 674-90. Date of Electronic Publication: 2013 Nov 13.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2985088R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1089-8638 (Electronic) Linking ISSN: 00222836 NLM ISO Abbreviation: J Mol Biol Subsets: MEDLINE
أسماء مطبوعة: Publication: Amsterdam : Elsevier
Original Publication: 1959- : London : Academic Press
مواضيع طبية MeSH: Intracellular Signaling Peptides and Proteins/*chemistry , Intracellular Signaling Peptides and Proteins/*metabolism , Neoplasm Proteins/*chemistry , Neoplasm Proteins/*metabolism , Ubiquitin/*metabolism, Amino Acid Motifs ; Amino Acid Sequence ; Crystallography, X-Ray ; Humans ; Magnetic Resonance Spectroscopy ; Molecular Sequence Data ; Protein Binding ; Protein Structure, Tertiary ; Scattering, Small Angle ; Sequence Homology, Amino Acid ; Signal Transduction
مستخلص: TAX1BP1 is a novel ubiquitin-binding adaptor protein involved in the negative regulation of the NF-kappaB transcription factor, which is a key player in inflammatory responses, immunity and tumorigenesis. TAX1BP1 recruits A20 to the ubiquitinated signaling proteins TRAF6 and RIP1, leading to their A20-mediated deubiquitination and the disruption of IL-1-induced and TNF-induced NF-kappaB signaling, respectively. The two zinc fingers localized at its C-terminus function as novel ubiquitin-binding domains (UBZ, ubiquitin-binding zinc finger). Here we present for the first time both the solution and crystal structures of two classical UBZ domains in tandem within the human TAX1BP1. The relative orientation of the two domains is slightly different in the X-ray structure with respect to the NMR structure, indicating some degree of conformational flexibility, which is rationalized by NMR relaxation data. The observed degree of flexibility and stability between the two UBZ domains might have consequences on the recognition mechanism of interacting partners.
(Copyright © 2013 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: 2D; 3D; NMR; NOE; SAXS; X-ray; hetNOE; heteronuclear NOE; inflammation; nuclear Overhauser enhancement; small-angle X-ray scattering; three-dimensional; two-dimensional; ubiquitin-binding domains
المشرفين على المادة: 0 (Intracellular Signaling Peptides and Proteins)
0 (Neoplasm Proteins)
0 (TAX1BP1 protein, human)
0 (Ubiquitin)
تواريخ الأحداث: Date Created: 20131119 Date Completed: 20140325 Latest Revision: 20140120
رمز التحديث: 20221213
DOI: 10.1016/j.jmb.2013.11.006
PMID: 24239949
قاعدة البيانات: MEDLINE
الوصف
تدمد:1089-8638
DOI:10.1016/j.jmb.2013.11.006