التفاصيل البيبلوغرافية
| العنوان: |
Novel parallelized quadrupole/linear ion trap/Orbitrap tribrid mass spectrometer improving proteome coverage and peptide identification rates. |
| المؤلفون: |
Senko MW; Thermo Fisher Scientific , 355 River Oaks Parkway, San Jose, California 95134, United States., Remes PM, Canterbury JD, Mathur R, Song Q, Eliuk SM, Mullen C, Earley L, Hardman M, Blethrow JD, Bui H, Specht A, Lange O, Denisov E, Makarov A, Horning S, Zabrouskov V |
| المصدر: |
Analytical chemistry [Anal Chem] 2013 Dec 17; Vol. 85 (24), pp. 11710-4. Date of Electronic Publication: 2013 Nov 27. |
| نوع المنشور: |
Journal Article |
| اللغة: |
English |
| بيانات الدورية: |
Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6882 (Electronic) Linking ISSN: 00032700 NLM ISO Abbreviation: Anal Chem Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: Washington, American Chemical Society. |
| مواضيع طبية MeSH: |
Mass Spectrometry/*methods , Peptides/*analysis , Proteomics/*methods, Mass Spectrometry/instrumentation ; Peptides/chemistry ; Proteomics/instrumentation ; Time Factors |
| مستخلص: |
Proteome coverage and peptide identification rates have historically advanced in line with improvements to the detection limits and acquisition rate of the mass spectrometer. For a linear ion trap/Orbitrap hybrid, the acquisition rate has been limited primarily by the duration of the ion accumulation and analysis steps. It is shown here that the spectral acquisition rate can be significantly improved through extensive parallelization of the acquisition process using a novel mass spectrometer incorporating quadrupole, Orbitrap, and linear trap analyzers. Further, these improvements to the acquisition rate continue to enhance proteome coverage and general experimental throughput. |
| المشرفين على المادة: |
0 (Peptides) |
| تواريخ الأحداث: |
Date Created: 20131121 Date Completed: 20140715 Latest Revision: 20131217 |
| رمز التحديث: |
20221213 |
| DOI: |
10.1021/ac403115c |
| PMID: |
24251866 |
| قاعدة البيانات: |
MEDLINE |
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