دورية أكاديمية
Polysaccharides from Ganoderma formosanum function as a Th1 adjuvant and stimulate cytotoxic T cell response in vivo.
| العنوان: | Polysaccharides from Ganoderma formosanum function as a Th1 adjuvant and stimulate cytotoxic T cell response in vivo. |
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| المؤلفون: | Pi CC; Department of Biochemical Science and Technology, National Taiwan University, Taipei 106, Taiwan, ROC., Chu CL; Graduate Institute of Immunology, National Taiwan University, College of Medicine, Taipei 100, Taiwan, ROC., Lu CY; Department of Biochemical Science and Technology, National Taiwan University, Taipei 106, Taiwan, ROC., Zhuang YJ; Department of Biochemical Science and Technology, National Taiwan University, Taipei 106, Taiwan, ROC., Wang CL; Department of Biochemical Science and Technology, National Taiwan University, Taipei 106, Taiwan, ROC., Yu YH; Department of Biochemical Science and Technology, National Taiwan University, Taipei 106, Taiwan, ROC., Wang HY; Department of Biochemical Science and Technology, National Taiwan University, Taipei 106, Taiwan, ROC., Lin CC; Department of Biochemical Science and Technology, National Taiwan University, Taipei 106, Taiwan, ROC., Chen CJ; Department of Biochemical Science and Technology, National Taiwan University, Taipei 106, Taiwan, ROC. Electronic address: cjchen1@ntu.edu.tw. |
| المصدر: | Vaccine [Vaccine] 2014 Jan 09; Vol. 32 (3), pp. 401-8. Date of Electronic Publication: 2013 Nov 17. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 8406899 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2518 (Electronic) Linking ISSN: 0264410X NLM ISO Abbreviation: Vaccine Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam, The Netherlands : Elsevier Science Original Publication: [Guildford, Surrey, UK] : Butterworths, [c1983- |
| مواضيع طبية MeSH: | Adjuvants, Immunologic/*pharmacology , Ganoderma/*chemistry , Polysaccharides/*pharmacology , T-Lymphocytes, Cytotoxic/*immunology , Th1 Cells/*immunology, Adjuvants, Immunologic/isolation & purification ; Animals ; Dendritic Cells/drug effects ; Dendritic Cells/immunology ; Interferon-gamma/metabolism ; Male ; Mice ; Mice, Inbred C57BL ; Ovalbumin/administration & dosage ; Ovalbumin/immunology ; Polysaccharides/isolation & purification ; T-Lymphocytes, Cytotoxic/drug effects ; Taiwan ; Th1 Cells/drug effects ; Vaccines/administration & dosage ; Vaccines/immunology |
| مستخلص: | The fungus of Ganoderma is a basidiomycete that possesses a variety of pharmacological effects and has been used in traditional Asian medicine for centuries. Ganoderma formosanum is a native Ganoderma species isolated in Taiwan, and we have previously demonstrated that PS-F2, a polysaccharide fraction purified from the submerged culture broth of G. formosanum, exhibits immunostimulatory properties in macrophages. In this study, we further characterized the adjuvant functions of PS-F2. In vitro, PS-F2 stimulated dendritic cells (DCs) to produce proinflammatory cytokines, including TNF-α, interleukin (IL)-6, and IL-12/IL-23 p40. PS-F2 also stimulated DCs to express the maturation markers CD40, CD80, CD86, and MHC class II. In a murine splenocyte culture, PS-F2 treatment resulted in elevated expression of T-bet and interferon (IFN)-γ in T lymphocytes. When used as an adjuvant in vivo with the ovalbumin (OVA) antigen, PS-F2 stimulated OVA-specific antibody production and primed IFN-γ production in OVA-specific T lymphocytes. PS-F2-adjuvated immunization also induced OVA-specific CTLs, which protected mice from a challenge with tumor cells expressing OVA. Collectively, our data show that PS-F2 functions as an adjuvant capable of inducing a Th1-polarized adaptive immune response, which would be useful in vaccines against viruses and tumors. (Copyright © 2013 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Adjuvant; CTL response; Ganoderma formosanum; Polysaccharides; Th1 response; Vaccine |
| المشرفين على المادة: | 0 (Adjuvants, Immunologic) 0 (Polysaccharides) 0 (Vaccines) 82115-62-6 (Interferon-gamma) 9006-59-1 (Ovalbumin) |
| تواريخ الأحداث: | Date Created: 20131121 Date Completed: 20140820 Latest Revision: 20201209 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1016/j.vaccine.2013.11.027 |
| PMID: | 24252697 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1873-2518 |
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| DOI: | 10.1016/j.vaccine.2013.11.027 |