دورية أكاديمية
أعرض في EDS

Method for site-specific detection of m6A nucleoside presence in RNA based on high-resolution melting (HRM) analysis.

التفاصيل البيبلوغرافية
العنوان: Method for site-specific detection of m6A nucleoside presence in RNA based on high-resolution melting (HRM) analysis.
المؤلفون: Golovina AY; Department of Chemistry, Lomonosov Moscow State University, Moscow 119992, Russia, Department of Bioinformatics and Bioengineering, Lomonosov Moscow State University, Moscow 119992, Russia and A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia., Dzama MM, Petriukov KS, Zatsepin TS, Sergiev PV, Bogdanov AA, Dontsova OA
المصدر: Nucleic acids research [Nucleic Acids Res] 2014 Feb; Vol. 42 (4), pp. e27. Date of Electronic Publication: 2013 Nov 21.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE
أسماء مطبوعة: Publication: 1992- : Oxford : Oxford University Press
Original Publication: London, Information Retrieval ltd.
مواضيع طبية MeSH: Adenosine/*chemistry , Nucleic Acid Hybridization/*methods , RNA/*chemistry, Adenosine/analysis ; Adenosine/metabolism ; HEK293 Cells ; Humans ; Methylation ; Methyltransferases/genetics ; Oligonucleotide Probes ; RNA/metabolism
مستخلص: Chemical landscape of natural RNA species is decorated with the large number of modified nucleosides. Some of those could easily be detected by reverse transcription, while others permit only high-performance liquid chromatography or mass-spectrometry detection. Presence of m(6)A nucleoside at a particular position of long RNA molecule is challenging to observe. Here we report an easy and high-throughput method for detection of m(6)A nucleosides in RNA based on high-resolution melting analysis. The method relies on the previous knowledge of the modified nucleoside position at a particular place of RNA and allows rapid screening for conditions or genes necessary for formation of that modification.
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المشرفين على المادة: 0 (Oligonucleotide Probes)
63231-63-0 (RNA)
EC 2.1.1.- (Methyltransferases)
K72T3FS567 (Adenosine)
تواريخ الأحداث: Date Created: 20131123 Date Completed: 20140507 Latest Revision: 20211021
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC3936739
DOI: 10.1093/nar/gkt1160
PMID: 24265225
قاعدة البيانات: MEDLINE
الوصف
تدمد:1362-4962
DOI:10.1093/nar/gkt1160