دورية أكاديمية
Osteogenesis induction of periodontal ligament cells onto bone morphogenic protein-2 immobilized PCL fibers.
| العنوان: | Osteogenesis induction of periodontal ligament cells onto bone morphogenic protein-2 immobilized PCL fibers. |
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| المؤلفون: | Kim SE; Department of Orthopedic Surgery & Rare Diseases Institute, Korea University Medical Center, Guro Hospital, #148, Gurodong-ro, Guro-gu, Seoul 152-703, Republic of Korea., Yun YP, Han YK, Lee DW, Ohe JY, Lee BS, Song HR, Park K, Choi BJ |
| المصدر: | Carbohydrate polymers [Carbohydr Polym] 2014 Jan; Vol. 99, pp. 700-9. Date of Electronic Publication: 2013 Aug 28. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 8307156 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1344 (Electronic) Linking ISSN: 01448617 NLM ISO Abbreviation: Carbohydr Polym Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <1992-> : Barking : Elsevier Applied Science Publishers Original Publication: London [Eng.] : Applied Science Publishers, c1981- |
| مواضيع طبية MeSH: | Tissue Scaffolds*, Bone Morphogenetic Protein 2/*pharmacology , Dopamine/*chemistry , Heparin/*chemistry , Periodontal Ligament/*drug effects , Polyesters/*chemistry, Alkaline Phosphatase/genetics ; Alkaline Phosphatase/metabolism ; Bone Morphogenetic Protein 2/genetics ; Bone Morphogenetic Protein 2/metabolism ; Calcium/metabolism ; Cell Differentiation ; Cell Proliferation ; Dopamine/pharmacology ; Gene Expression ; Heparin/pharmacology ; Humans ; Immobilized Proteins ; Osteogenesis/drug effects ; Periodontal Ligament/cytology ; Periodontal Ligament/metabolism ; Photoelectron Spectroscopy ; Primary Cell Culture ; Tissue Engineering |
| مستخلص: | The purpose of this study was to develop bone morphogenic protein-2 (BMP-2) immobilized PCL fibers to induce osteogenic differentiation of periodontal ligament cells (PDLCs). The PCL fiber surface was modified with heparin-dopamine (Hep-DOPA) (Hep-PCL) and further immobilized with BMP-2 (BMP-2/Hep-PCL). PCL fibers and surface-modified PCL fibers (Hep-PCL and BMP-2/Hep-PCL) were characterized by X-ray photoelectron spectroscopy (XPS) and contact angle. Osteogenic differentiation of PDLCs was demonstrated by alkaline phosphatase (ALP) activity, calcium deposition, and gene expression. The results of XPS and contact angle revealed that Hep-DOPA and BMP-2 were successfully immobilized onto the PCL surface and that the BMP-2/Hep-PCL fibers have more hydrophilic surface properties than PCL fibers alone. ALP activity, calcium deposition, and gene expression on BMP-2/Hep-PCL fibers showed significantly induced osteogenic differentiation relative to PCL fibers. Therefore, we suggest that BMP-2/Hep-PCL fibers have the potential to effectively induce osteogenic differentiation of PDLCs. (Copyright © 2013. Published by Elsevier Ltd.) |
| فهرسة مساهمة: | Keywords: Bone morphogenic protein-2 (BMP-2); Osteogenesis induction; PCL fiber; Periodontal ligament cells (PDLCs) |
| المشرفين على المادة: | 0 (BMP2 protein, human) 0 (Bone Morphogenetic Protein 2) 0 (Immobilized Proteins) 0 (Polyesters) 24980-41-4 (polycaprolactone) 9005-49-6 (Heparin) EC 3.1.3.1 (Alkaline Phosphatase) SY7Q814VUP (Calcium) VTD58H1Z2X (Dopamine) |
| تواريخ الأحداث: | Date Created: 20131127 Date Completed: 20140714 Latest Revision: 20220318 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.carbpol.2013.08.053 |
| PMID: | 24274561 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-1344 |
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| DOI: | 10.1016/j.carbpol.2013.08.053 |