دورية أكاديمية
أعرض في EDS

Full inactivation of human influenza virus by high hydrostatic pressure preserves virus structure and membrane fusion while conferring protection to mice against infection.

التفاصيل البيبلوغرافية
العنوان: Full inactivation of human influenza virus by high hydrostatic pressure preserves virus structure and membrane fusion while conferring protection to mice against infection.
المؤلفون: Dumard CH; Instituto de Bioquímica Médica and Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil., Barroso SP, de Oliveira GA, Carvalho CA, Gomes AM, Couceiro JN, Ferreira DF, Nico D, Oliveira AC, Silva JL, Santos PS
المصدر: PloS one [PLoS One] 2013 Nov 25; Vol. 8 (11), pp. e80785. Date of Electronic Publication: 2013 Nov 25 (Print Publication: 2013).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Hydrostatic Pressure* , Membrane Fusion*, Influenza, Human/*virology , Orthomyxoviridae/*physiology , Orthomyxoviridae Infections/*prevention & control, Animals ; Antibodies, Viral/biosynthesis ; Humans ; Mice ; Microscopy, Electron ; Orthomyxoviridae/immunology ; Orthomyxoviridae/ultrastructure ; Orthomyxoviridae Infections/virology
مستخلص: Whole inactivated vaccines (WIVs) possess greater immunogenicity than split or subunit vaccines, and recent studies have demonstrated that WIVs with preserved fusogenic activity are more protective than non-fusogenic WIVs. In this work, we describe the inactivation of human influenza virus X-31 by high hydrostatic pressure (HHP) and analyze the effects on the structure by spectroscopic measurements, light scattering, and electron microscopy. We also investigated the effects of HHP on the glycoprotein activity and fusogenic activity of the viral particles. The electron microscopy data showed pore formation on the viral envelope, but the general morphology was preserved, and small variations were seen in the particle structure. The activity of hemagglutinin (HA) during the process of binding and fusion was affected in a time-dependent manner, but neuraminidase (NA) activity was not affected. Infectious activity ceased after 3 hours of pressurization, and mice were protected from infection after being vaccinated. Our results revealed full viral inactivation with overall preservation of viral structure and maintenance of fusogenic activity, thereby conferring protection against infection. A strong response consisting of serum immunoglobulin IgG1, IgG2a, and serum and mucosal IgA was also detected after vaccination. Thus, our data strongly suggest that applying hydrostatic pressure may be an effective method for developing new vaccines against influenza A as well as other viruses.
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المشرفين على المادة: 0 (Antibodies, Viral)
تواريخ الأحداث: Date Created: 20131128 Date Completed: 20140805 Latest Revision: 20211021
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC3840014
DOI: 10.1371/journal.pone.0080785
PMID: 24282553
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0080785