دورية أكاديمية
Chlorella modulates insulin signaling pathway and prevents high-fat diet-induced insulin resistance in mice.
| العنوان: | Chlorella modulates insulin signaling pathway and prevents high-fat diet-induced insulin resistance in mice. |
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| المؤلفون: | Vecina JF; Department of Pharmacology/Hemocenter, State University of Campinas, Campinas, SP 13083-970, Brazil., Oliveira AG; Department of Internal Medicine, State University of Campinas, Campinas, SP 13081-970, Brazil., Araujo TG; Department of Internal Medicine, State University of Campinas, Campinas, SP 13081-970, Brazil., Baggio SR; Food Science and Quality Center (CCQA), Food Technology Institute (ITAL), Campinas, SP 13070-178, Brazil., Torello CO; Department of Pharmacology/Hemocenter, State University of Campinas, Campinas, SP 13083-970, Brazil., Saad MJ; Department of Internal Medicine, State University of Campinas, Campinas, SP 13081-970, Brazil., Queiroz ML; Department of Pharmacology/Hemocenter, State University of Campinas, Campinas, SP 13083-970, Brazil. Electronic address: mlsq@uol.com.br. |
| المصدر: | Life sciences [Life Sci] 2014 Jan 24; Vol. 95 (1), pp. 45-52. Date of Electronic Publication: 2013 Dec 11. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0375521 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0631 (Electronic) Linking ISSN: 00243205 NLM ISO Abbreviation: Life Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2008->: Amsterdam : Elsevier Original Publication: Oxford; Elmsford, N. Y. [etc.] Pergamon Press. |
| مواضيع طبية MeSH: | Insulin Resistance*, Chlorella vulgaris/*chemistry , Insulin/*metabolism , Obesity/*drug therapy , Plant Extracts/*pharmacology, Adipose Tissue/drug effects ; Adipose Tissue/metabolism ; Animals ; Blood Glucose/drug effects ; Body Weight/drug effects ; Diet, High-Fat/adverse effects ; Dyslipidemias/etiology ; Dyslipidemias/prevention & control ; Glucose Tolerance Test ; Leptin/metabolism ; Lipids/blood ; Liver/drug effects ; Liver/metabolism ; Male ; Mice ; Mice, Inbred BALB C ; Muscle, Skeletal/drug effects ; Muscle, Skeletal/metabolism ; Obesity/complications ; Phosphorylation/drug effects ; Signal Transduction/drug effects |
| مستخلص: | Aims: The search for natural agents that minimize obesity-associated disorders is receiving special attention. In this regard, the present study aimed to evaluate the prophylactic effect of Chlorella vulgaris (CV) on body weight, lipid profile, blood glucose and insulin signaling in liver, skeletal muscle and adipose tissue of diet-induced obese mice. Main Methods: Balb/C mice were fed either with standard rodent chow diet or high-fat diet (HFD) and received concomitant treatment with CV for 12 consecutive weeks. Triglyceride, free fatty acid, total cholesterol and fractions of cholesterol were measured using commercial assay. Insulin and leptin levels were determined by enzyme-linked immunosorbent assay (ELISA). Insulin and glucose tolerance tests were performed. The expression and phosphorylation of IRβ, IRS-1 and Akt were determined by Western blot analyses. Key Findings: Herein we demonstrate for the first time in the literature that prevention by CV of high-fat diet-induced insulin resistance in obese mice, as shown by increased glucose and insulin tolerance, is in part due to the improvement in the insulin signaling pathway at its main target tissues, by increasing the phosphorylation levels of proteins such as IR, IRS-1 and Akt. In parallel, the lower phosphorylation levels of IRS-1(ser307) were observed in obese mice. We also found that CV administration prevents high-fat diet-induced dyslipidemia by reducing triglyceride, cholesterol and free fatty acid levels. Significance: We propose that the modulatory effect of CV treatment preventing the deleterious effects induced by high-fat diet is a good indicator for its use as a prophylactic-therapeutic agent against obesity-related complications. (Copyright © 2013 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Chlorella vulgaris; Insulin resistance; Insulin signaling pathway; Obesity |
| المشرفين على المادة: | 0 (Blood Glucose) 0 (Insulin) 0 (Leptin) 0 (Lipids) 0 (Plant Extracts) |
| تواريخ الأحداث: | Date Created: 20131217 Date Completed: 20140305 Latest Revision: 20140113 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.lfs.2013.11.020 |
| PMID: | 24333277 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0631 |
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| DOI: | 10.1016/j.lfs.2013.11.020 |