دورية أكاديمية
Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica.
| العنوان: | Generation and selection of anti-flagellin monoclonal antibodies useful for serotyping Salmonella enterica. |
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| المؤلفون: | Hiriart Y; Laboratorio de Investigaciones del Sistema Inmune (LISIN), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, CP1900 Argentina., Serradell M; Departamento de Ciencias Biológicas, Cátedra de Microbiología, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina., Martínez A; Departamento de Bacteriología y Virología, Instituto de Higiene, Centro Nacional de Salmonella, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay., Sampaolesi S; Departamento de Ciencias Biológicas, Cátedra de Microbiología, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina., Maciel DG; Laboratorio de Investigaciones del Sistema Inmune (LISIN), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, CP1900 Argentina., Chabalgoity JA; Departamento de Desarrollo Biotecnológico, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay., Yim L; Departamento de Desarrollo Biotecnológico, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay., Algorta G; Departamento de Bacteriología y Virología, Instituto de Higiene, Centro Nacional de Salmonella, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay., Rumbo M; Laboratorio de Investigaciones del Sistema Inmune (LISIN), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, CP1900 Argentina. |
| المصدر: | SpringerPlus [Springerplus] 2013 Nov 28; Vol. 2, pp. 640. Date of Electronic Publication: 2013 Nov 28 (Print Publication: 2013). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: SpringerPlus Country of Publication: Switzerland NLM ID: 101597967 Publication Model: eCollection Cited Medium: Print ISSN: 2193-1801 (Print) Linking ISSN: 21931801 NLM ISO Abbreviation: Springerplus Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Switzerland : SpringerPlus |
| مستخلص: | In developing countries, bacterial acute gastroenteritis continues to be an important cause of morbidity and mortality among young children. Salmonellosis constitutes a major cause of infectious enteritis worldwide, most of them associated to the consumption of contaminated food products. Traditionally, Salmonella has been classified in serovars based on varieties of O and H surface antigens. In the present work we generated and characterized a panel of anti-flagellin monoclonal antibodies (MAbs) in order to select antibodies useful for detecting the H surface antigen. Four different MAbs were obtained by somatic hybridization of splenocytes. We found two MAbs that recognised regions of flagellin conserved among different Salmonella serovars. Other two MAbs recognised structures restricted to Salmonella enterica sv. Typhimurium, being one of them suitable for agglutination tests. Using a diverse panel of S. enterica serovars with different H antigen varieties we confirmed that this MAb agglutinates specifically S. Typhimurium (antigenic formula: 4,12:i:1,2) or other serovars expressing flagellar factor i. In conclusion, we generated a valuable immunochemical tool to be used in simple assays for serotyping of epidemiologically relevant strains. The capacity to characterize specific strains and determine the primary sources of Salmonella contamination generate valuable information of the epidemiology of this microorganism, contributing to the improvement of public health. |
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| فهرسة مساهمة: | Keywords: Flagellin; Monoclonal antibodies; Salmonella; Serotyping |
| تواريخ الأحداث: | Date Created: 20131219 Date Completed: 20131218 Latest Revision: 20211021 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC3862864 |
| DOI: | 10.1186/2193-1801-2-640 |
| PMID: | 24349948 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 2193-1801 |
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| DOI: | 10.1186/2193-1801-2-640 |