دورية أكاديمية
Tissue-specific gene silencing monitored in circulating RNA.
العنوان: | Tissue-specific gene silencing monitored in circulating RNA. |
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المؤلفون: | Sehgal A, Chen Q, Gibbings D, Sah DW, Bumcrot D |
المصدر: | RNA (New York, N.Y.) [RNA] 2014 Feb; Vol. 20 (2), pp. 143-9. Date of Electronic Publication: 2013 Dec 19. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Cold Spring Harbor Laboratory Press Country of Publication: United States NLM ID: 9509184 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-9001 (Electronic) Linking ISSN: 13558382 NLM ISO Abbreviation: RNA Subsets: MEDLINE |
أسماء مطبوعة: | Publication: <2003->: Cold Spring Harbor, NY : Cold Spring Harbor Laboratory Press Original Publication: New York, NY : Cambridge University Press, c1995- |
مواضيع طبية MeSH: | Gene Knockdown Techniques* , RNA Interference*, RNA, Messenger/*blood, Aged ; Animals ; Biomarkers, Tumor/blood ; Biomarkers, Tumor/genetics ; Gene Expression ; Glypicans/genetics ; Humans ; Liver/metabolism ; Macaca fascicularis ; Male ; Organ Specificity ; RNA, Messenger/genetics ; RNA, Small Interfering/genetics ; Rats ; Rats, Sprague-Dawley ; Species Specificity ; alpha-Fetoproteins/genetics |
مستخلص: | Pharmacologic target gene modulation is the primary objective for RNA antagonist strategies and gene therapy. Here we show that mRNAs encoding tissue-specific gene transcripts can be detected in biological fluids and that RNAi-mediated target gene silencing in the liver and brain results in quantitative reductions in serum and cerebrospinal fluid mRNA levels, respectively. Further, administration of an anti-miRNA oligonucleotide resulted in decreased levels of the miRNA in circulation. Moreover, ectopic expression of an adenoviral transgene in the liver was quantified based on measurement of serum mRNA levels. This noninvasive method for monitoring tissue-specific RNA modulation could greatly advance the clinical development of RNA-based therapeutics. |
References: | J Proteome Res. 2008 Dec;7(12):5157-66. (PMID: 19367702) Biol Direct. 2007 Nov 30;2:35. (PMID: 18053135) Cerebrospinal Fluid Res. 2009 Sep 07;6:10. (PMID: 19735572) Nature. 2005 Dec 1;438(7068):685-9. (PMID: 16258535) Arch Virol. 2004 Aug;149(8):1611-7. (PMID: 15290384) Biochem Pharmacol. 2011 May 15;81(10):1171-82. (PMID: 21371441) Nature. 2006 May 4;441(7089):111-4. (PMID: 16565705) Proc Natl Acad Sci U S A. 2010 Feb 2;107(5):1864-9. (PMID: 20080679) Proc Natl Acad Sci U S A. 2008 Aug 19;105(33):11915-20. (PMID: 18695239) Nature. 2010 Apr 15;464(7291):1067-70. (PMID: 20305636) Cell Metab. 2006 Feb;3(2):87-98. (PMID: 16459310) Mol Neurodegener. 2008 Nov 01;3:19. (PMID: 18976489) Cancer Res. 2011 Jun 1;71(11):3792-801. (PMID: 21478294) PLoS One. 2008;3(11):e3694. (PMID: 19002258) Mol Ther. 2010 Jul;18(7):1357-64. (PMID: 20461061) Oligonucleotides. 2009 Mar;19(1):23-29. (PMID: 19093781) Semin Oncol. 2012 Aug;39(4):410-33. (PMID: 22846859) Ann Clin Biochem. 2003 Mar;40(Pt 2):122-30. (PMID: 12662399) Nat Genet. 2008 May;40(5):569-71. (PMID: 18408718) Anticancer Res. 2007 May-Jun;27(3A):1257-65. (PMID: 17593617) N Engl J Med. 2013 Aug 29;369(9):819-29. (PMID: 23984729) Hum Mutat. 1995;5(3):191-6. (PMID: 7599630) Nature. 1997 Aug 28;388(6645):839-40. (PMID: 9278044) Clin Chem. 1972 Jun;18(6):519-22. (PMID: 5026765) Nat Cell Biol. 2007 Jun;9(6):654-9. (PMID: 17486113) Proteomics. 2011 Feb;11(4):709-20. (PMID: 21241021) |
فهرسة مساهمة: | Keywords: RNAi; circulating RNA; exosome; gene silencing; in vivo delivery |
المشرفين على المادة: | 0 (Biomarkers, Tumor) 0 (GPC3 protein, human) 0 (Glypicans) 0 (RNA, Messenger) 0 (RNA, Small Interfering) 0 (alpha-Fetoproteins) |
تواريخ الأحداث: | Date Created: 20131221 Date Completed: 20140318 Latest Revision: 20211021 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC3895267 |
DOI: | 10.1261/rna.042507.113 |
PMID: | 24355758 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1469-9001 |
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DOI: | 10.1261/rna.042507.113 |