دورية أكاديمية
Tissue-specific gene silencing monitored in circulating RNA.
| العنوان: | Tissue-specific gene silencing monitored in circulating RNA. |
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| المؤلفون: | Sehgal A, Chen Q, Gibbings D, Sah DW, Bumcrot D |
| المصدر: | RNA (New York, N.Y.) [RNA] 2014 Feb; Vol. 20 (2), pp. 143-9. Date of Electronic Publication: 2013 Dec 19. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cold Spring Harbor Laboratory Press Country of Publication: United States NLM ID: 9509184 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-9001 (Electronic) Linking ISSN: 13558382 NLM ISO Abbreviation: RNA Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2003->: Cold Spring Harbor, NY : Cold Spring Harbor Laboratory Press Original Publication: New York, NY : Cambridge University Press, c1995- |
| مواضيع طبية MeSH: | Gene Knockdown Techniques* , RNA Interference*, RNA, Messenger/*blood, Aged ; Animals ; Biomarkers, Tumor/blood ; Biomarkers, Tumor/genetics ; Gene Expression ; Glypicans/genetics ; Humans ; Liver/metabolism ; Macaca fascicularis ; Male ; Organ Specificity ; RNA, Messenger/genetics ; RNA, Small Interfering/genetics ; Rats ; Rats, Sprague-Dawley ; Species Specificity ; alpha-Fetoproteins/genetics |
| مستخلص: | Pharmacologic target gene modulation is the primary objective for RNA antagonist strategies and gene therapy. Here we show that mRNAs encoding tissue-specific gene transcripts can be detected in biological fluids and that RNAi-mediated target gene silencing in the liver and brain results in quantitative reductions in serum and cerebrospinal fluid mRNA levels, respectively. Further, administration of an anti-miRNA oligonucleotide resulted in decreased levels of the miRNA in circulation. Moreover, ectopic expression of an adenoviral transgene in the liver was quantified based on measurement of serum mRNA levels. This noninvasive method for monitoring tissue-specific RNA modulation could greatly advance the clinical development of RNA-based therapeutics. |
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| فهرسة مساهمة: | Keywords: RNAi; circulating RNA; exosome; gene silencing; in vivo delivery |
| المشرفين على المادة: | 0 (Biomarkers, Tumor) 0 (GPC3 protein, human) 0 (Glypicans) 0 (RNA, Messenger) 0 (RNA, Small Interfering) 0 (alpha-Fetoproteins) |
| تواريخ الأحداث: | Date Created: 20131221 Date Completed: 20140318 Latest Revision: 20211021 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC3895267 |
| DOI: | 10.1261/rna.042507.113 |
| PMID: | 24355758 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1469-9001 |
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| DOI: | 10.1261/rna.042507.113 |