دورية أكاديمية
أعرض في EDS

Membrane cholesterol removal changes mechanical properties of cells and induces secretion of a specific pool of lysosomes.

التفاصيل البيبلوغرافية
العنوان: Membrane cholesterol removal changes mechanical properties of cells and induces secretion of a specific pool of lysosomes.
المؤلفون: Hissa B; Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil., Pontes B; LPO-COPEA, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Roma PM; Departamento de Física, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil., Alves AP; Departamento de Física, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil., Rocha CD; Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil., Valverde TM; Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil., Aguiar PH; Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil., Almeida FP; Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Guimarães AJ; Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Universidade Federal Fluminense, Rio de Janeiro, RJ, Brazil., Guatimosim C; Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil., Silva AM; Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil., Fernandes MC; Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland, United States of America., Andrews NW; Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland, United States of America., Viana NB; LPO-COPEA, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil ; Instituto de Física, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Mesquita ON; Departamento de Física, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil., Agero U; Departamento de Física, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil., Andrade LO; Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
المصدر: PloS one [PLoS One] 2013 Dec 20; Vol. 8 (12), pp. e82988. Date of Electronic Publication: 2013 Dec 20 (Print Publication: 2013).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Cell Membrane/*drug effects , Cholesterol/*chemistry , Fibroblasts/*drug effects , Lysosomes/*metabolism , beta-Cyclodextrins/*pharmacology, Actins/genetics ; Actins/metabolism ; Animals ; Bridged Bicyclo Compounds, Heterocyclic/pharmacology ; Cell Line ; Cell Membrane/ultrastructure ; Cholesterol/deficiency ; Cytoskeleton/drug effects ; Cytoskeleton/ultrastructure ; Exocytosis/drug effects ; Fibroblasts/cytology ; Fibroblasts/metabolism ; Gene Expression ; Lysosomes/classification ; Membrane Fluidity/drug effects ; Mice ; RNA, Small Interfering/genetics ; RNA, Small Interfering/metabolism ; Synaptotagmins/antagonists & inhibitors ; Synaptotagmins/genetics ; Synaptotagmins/metabolism ; Thiazolidines/pharmacology ; rho GTP-Binding Proteins/genetics ; rho GTP-Binding Proteins/metabolism
مستخلص: In a previous study we had shown that membrane cholesterol removal induced unregulated lysosomal exocytosis events leading to the depletion of lysosomes located at cell periphery. However, the mechanism by which cholesterol triggered these exocytic events had not been uncovered. In this study we investigated the importance of cholesterol in controlling mechanical properties of cells and its connection with lysosomal exocytosis. Tether extraction with optical tweezers and defocusing microscopy were used to assess cell dynamics in mouse fibroblasts. These assays showed that bending modulus and surface tension increased when cholesterol was extracted from fibroblasts plasma membrane upon incubation with MβCD, and that the membrane-cytoskeleton relaxation time increased at the beginning of MβCD treatment and decreased at the end. We also showed for the first time that the amplitude of membrane-cytoskeleton fluctuation decreased during cholesterol sequestration, showing that these cells become stiffer. These changes in membrane dynamics involved not only rearrangement of the actin cytoskeleton, but also de novo actin polymerization and stress fiber formation through Rho activation. We found that these mechanical changes observed after cholesterol sequestration were involved in triggering lysosomal exocytosis. Exocytosis occurred even in the absence of the lysosomal calcium sensor synaptotagmin VII, and was associated with actin polymerization induced by MβCD. Notably, exocytosis triggered by cholesterol removal led to the secretion of a unique population of lysosomes, different from the pool mobilized by actin depolymerizing drugs such as Latrunculin-A. These data support the existence of at least two different pools of lysosomes with different exocytosis dynamics, one of which is directly mobilized for plasma membrane fusion after cholesterol removal.
References: Microbes Infect. 2010 Sep;12(10):784-9. (PMID: 20561595)
Theriogenology. 2008 Nov;70(8):1188-96. (PMID: 18640708)
J Cell Sci. 2012 Dec 15;125(Pt 24):5937-43. (PMID: 23038769)
Mol Biol Cell. 2006 Feb;17(2):977-89. (PMID: 16339081)
Neuron. 1995 Feb;14(2):353-63. (PMID: 7857644)
J Biol Chem. 2002 Nov 8;277(45):43399-409. (PMID: 12202484)
Proteomics. 2007 Jul;7(14):2398-409. (PMID: 17623299)
Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11875-80. (PMID: 23821745)
Cell. 2003 Feb 21;112(4):519-33. (PMID: 12600315)
J Exp Biol. 1988 Sep;139:253-66. (PMID: 3062121)
Cell Mol Life Sci. 2009 Jul;66(14):2319-28. (PMID: 19370312)
J Biol Chem. 1998 Aug 28;273(35):22298-304. (PMID: 9712847)
Semin Cell Dev Biol. 2007 Oct;18(5):616-26. (PMID: 17719248)
J Cell Sci. 2007 Jul 1;120(Pt 13):2223-31. (PMID: 17550968)
Proteomics. 2005 Dec;5(18):4733-42. (PMID: 16267816)
J Cell Biol. 2000 Mar 20;148(6):1141-49. (PMID: 10725327)
Ann N Y Acad Sci. 2002 Oct;971:222-31. (PMID: 12438122)
Proc Natl Acad Sci U S A. 2003 Nov 25;100(24):13964-9. (PMID: 14612561)
Anal Biochem. 1976 May 7;72:248-54. (PMID: 942051)
Biophys J. 2011 Jul 6;101(1):43-52. (PMID: 21723813)
Biochem Biophys Res Commun. 2005 Apr 1;329(1):117-24. (PMID: 15721282)
J Cell Sci. 2010 Feb 15;123(Pt 4):595-605. (PMID: 20103534)
EMBO J. 2001 May 1;20(9):2202-13. (PMID: 11331586)
J Cell Biol. 2006 Aug 28;174(5):725-34. (PMID: 16943184)
PLoS One. 2013 Jul 03;8(7):e67708. (PMID: 23844071)
J Cell Biol. 1995 Feb;128(4):589-98. (PMID: 7860632)
J Biol Chem. 2004 May 7;279(19):20471-9. (PMID: 14993220)
Endocrinology. 2008 Oct;149(10):5136-45. (PMID: 18599549)
PLoS Negl Trop Dis. 2012;6(3):e1583. (PMID: 22479662)
J Biol Chem. 1957 May;226(1):497-509. (PMID: 13428781)
FASEB J. 2009 Jan;23(1):58-67. (PMID: 18806218)
Biophys J. 1999 Oct;77(4):1992-2002. (PMID: 10512819)
Biochem Biophys Res Commun. 2010 Jan 1;391(1):414-8. (PMID: 19914217)
Cell Signal. 2003 Oct;15(10):893-9. (PMID: 12873702)
Biochim Biophys Acta. 2004 Dec 6;1742(1-3):37-49. (PMID: 15590054)
Neuron. 2000 Sep;27(3):539-50. (PMID: 11055436)
Cell. 2001 Jul 27;106(2):157-69. (PMID: 11511344)
PLoS One. 2013;8(2):e57147. (PMID: 23451167)
Phys Rev E Stat Nonlin Soft Matter Phys. 2003 May;67(5 Pt 1):051904. (PMID: 12786175)
Biophys J. 1990 Oct;58(4):997-1009. (PMID: 2249000)
Biomaterials. 2013 Jun;34(17):4309-26. (PMID: 23478037)
Soft Matter. 2012;8(32):8350-8360. (PMID: 23227105)
Biophys J. 2006 Aug 1;91(3):1108-15. (PMID: 16617074)
Antioxid Redox Signal. 2007 Sep;9(9):1519-34. (PMID: 17576163)
J Neurosci. 1992 Jun;12(6):2186-97. (PMID: 1607935)
Cell Mol Bioeng. 2010 Jun 1;3(2):151-162. (PMID: 21461187)
Biophys J. 2004 Nov;87(5):3336-43. (PMID: 15347591)
Cell Motil Cytoskeleton. 2001 Jan;48(1):37-51. (PMID: 11124709)
Microsc Res Tech. 2004 Oct;65(3):159-65. (PMID: 15605417)
PLoS Biol. 2004 Aug;2(8):E233. (PMID: 15226824)
Nature. 2012 Oct 11;490(7419):201-7. (PMID: 23060190)
J Physiol. 2006 Feb 15;571(Pt 1):83-99. (PMID: 16339182)
Biochemistry. 1988 Oct 18;27(21):8261-9. (PMID: 3233209)
J Physiol. 2007 Mar 1;579(Pt 2):413-29. (PMID: 17170046)
Biochim Biophys Acta. 2007 Jun;1768(6):1311-24. (PMID: 17493580)
PLoS One. 2011;6(12):e29162. (PMID: 22195014)
Mol Biol Cell. 2009 Jul;20(14):3261-72. (PMID: 19458190)
Cell Mol Bioeng. 2012 Jun 1;5(2):143-154. (PMID: 23487555)
Ann N Y Acad Sci. 2009 Jan;1152:43-52. (PMID: 19161375)
Mol Membr Biol. 2007 Jul-Aug;24(4):313-24. (PMID: 17520487)
J Cell Biol. 2005 Oct 10;171(1):121-31. (PMID: 16203859)
Mol Biol Cell. 2004 Jul;15(7):3132-45. (PMID: 15121881)
J Cell Biochem. 2009 Apr 15;106(6):1031-40. (PMID: 19229870)
J Cell Biol. 2010 Jun 14;189(6):1027-38. (PMID: 20530211)
Cell. 2012 Sep 14;150(6):1300, 1300.e1-2. (PMID: 22980986)
Cell Mol Biol Lett. 2001;6(3):703-20. (PMID: 11598643)
J Cell Biol. 2002 Nov 25;159(4):625-35. (PMID: 12438417)
J Mol Neurosci. 2012 Oct;48(2):328-38. (PMID: 22588981)
HFSP J. 2010 Apr;4(2):85-92. (PMID: 20885775)
Nat Rev Mol Cell Biol. 2001 Feb;2(2):98-106. (PMID: 11252968)
Proc Natl Acad Sci U S A. 2011 Aug 30;108(35):14467-72. (PMID: 21808040)
J Biol Chem. 2005 May 20;280(20):19449-53. (PMID: 15769746)
PLoS One. 2010 Nov 29;5(11):e15054. (PMID: 21124786)
معلومات مُعتمدة: R01 GM064625 United States GM NIGMS NIH HHS
المشرفين على المادة: 0 (Actins)
0 (Bridged Bicyclo Compounds, Heterocyclic)
0 (RNA, Small Interfering)
0 (Thiazolidines)
0 (beta-Cyclodextrins)
0 (methyl-beta-cyclodextrin)
134193-27-4 (Synaptotagmins)
97C5T2UQ7J (Cholesterol)
EC 3.6.5.2 (rho GTP-Binding Proteins)
SRQ9WWM084 (latrunculin A)
تواريخ الأحداث: Date Created: 20131231 Date Completed: 20141006 Latest Revision: 20220309
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC3869752
DOI: 10.1371/journal.pone.0082988
PMID: 24376622
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0082988