دورية أكاديمية
أعرض في EDS

Staged miRNA re-regulation patterns during reprogramming.

التفاصيل البيبلوغرافية
العنوان: Staged miRNA re-regulation patterns during reprogramming.
المؤلفون: Henzler CM, Li Z, Dang J, Arcila ML, Zhou H, Liu J, Chang KY, Bassett DS, Rana TM, Kosik KS
المصدر: Genome biology [Genome Biol] 2013 Dec 31; Vol. 14 (12), pp. R149. Date of Electronic Publication: 2013 Dec 31.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: BioMed Central Ltd Country of Publication: England NLM ID: 100960660 Publication Model: Electronic Cited Medium: Internet ISSN: 1474-760X (Electronic) Linking ISSN: 14747596 NLM ISO Abbreviation: Genome Biol Subsets: MEDLINE
أسماء مطبوعة: Publication: London, UK : BioMed Central Ltd
Original Publication: London : Genome Biology Ltd., c2000-
مواضيع طبية MeSH: Cellular Reprogramming* , Gene Expression Regulation*, Induced Pluripotent Stem Cells/*metabolism , MicroRNAs/*genetics, Animals ; Calcium-Binding Proteins ; Cells, Cultured ; Fibroblasts/cytology ; Fibroblasts/metabolism ; High-Throughput Nucleotide Sequencing/methods ; Intercellular Signaling Peptides and Proteins/genetics ; Iodide Peroxidase/genetics ; Mice ; Transcription Factors/metabolism
مستخلص: Background: MiRNAs often operate in feedback loops with transcription factors and represent a key mechanism for fine-tuning gene expression. In transcription factor-induced reprogramming, miRNAs play a critical role; however, detailed analyses of miRNA expression changes during reprogramming at the level of deep sequencing have not been previously reported.
Results: We use four factor reprogramming to induce pluripotent stem cells from mouse fibroblasts and isolate FACS-sorted Thy1- and SSEA1+ intermediates and Oct4-GFP+ induced pluripotent stem cells (iPSCs). Small RNAs from these cells, and two partial-iPSC lines, another iPSC line, and mouse embryonic stem cells (mES cells) were deep sequenced. A comprehensive resetting of the miRNA profile occurs during reprogramming; however, analysis of miRNA co-expression patterns yields only a few patterns of change. Dlk1-Dio3 region miRNAs dominate the large pool of miRNAs experiencing small but significant fold changes early in reprogramming. Overexpression of Dlk1-Dio3 miRNAs early in reprogramming reduces reprogramming efficiency, suggesting the observed downregulation of these miRNAs may contribute to reprogramming. As reprogramming progresses, fewer miRNAs show changes in expression, but those changes are generally of greater magnitude.
Conclusions: The broad resetting of the miRNA profile during reprogramming that we observe is due to small changes in gene expression in many miRNAs early in the process, and large changes in only a few miRNAs late in reprogramming. This corresponds with a previously observed transition from a stochastic to a more deterministic signal.
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المشرفين على المادة: 0 (Calcium-Binding Proteins)
0 (Dlk1 protein, mouse)
0 (Intercellular Signaling Peptides and Proteins)
0 (MicroRNAs)
0 (Transcription Factors)
EC 1.11.1.- (iodothyronine deiodinase type III)
EC 1.11.1.8 (Iodide Peroxidase)
تواريخ الأحداث: Date Created: 20140102 Date Completed: 20150423 Latest Revision: 20211021
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC4053856
DOI: 10.1186/gb-2013-14-12-r149
PMID: 24380417
قاعدة البيانات: MEDLINE
الوصف
تدمد:1474-760X
DOI:10.1186/gb-2013-14-12-r149