دورية أكاديمية
Closed loop adaptive optics for microscopy without a wavefront sensor.
| العنوان: | Closed loop adaptive optics for microscopy without a wavefront sensor. |
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| المؤلفون: | Kner P; Faculty of Engineering, University of Georgia, Athens, GA 30602., Winoto L; Keck Advanced Microscopy Laboratory and Department of Biochemistry and Biophysics, University of California San Francisco, 600 16 St., San Francisco, CA 94158., Agard DA; Keck Advanced Microscopy Laboratory and Department of Biochemistry and Biophysics, University of California San Francisco, 600 16 St., San Francisco, CA 94158 ; Howard Hughes Medical Institute, University of California, San Francisco, CA 94143., Sedat JW; Keck Advanced Microscopy Laboratory and Department of Biochemistry and Biophysics, University of California San Francisco, 600 16 St., San Francisco, CA 94158. |
| المصدر: | Proceedings of SPIE--the International Society for Optical Engineering [Proc SPIE Int Soc Opt Eng] 2010 Feb 24; Vol. 7570. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: SPIE, The International Society for Optical Engineering Country of Publication: United States NLM ID: 101524122 Publication Model: Print Cited Medium: Print ISSN: 0277-786X (Print) Linking ISSN: 0277786X NLM ISO Abbreviation: Proc SPIE Int Soc Opt Eng Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Bellingham, WA : SPIE, The International Society for Optical Engineering |
| مستخلص: | A three-dimensional wide-field image of a small fluorescent bead contains more than enough information to accurately calculate the wavefront in the microscope objective back pupil plane using the phase retrieval technique. The phase-retrieved wavefront can then be used to set a deformable mirror to correct the point-spread function (PSF) of the microscope without the use of a wavefront sensor. This technique will be useful for aligning the deformable mirror in a widefield microscope with adaptive optics and could potentially be used to correct aberrations in samples where small fluorescent beads or other point sources are used as reference beacons. Another advantage is the high resolution of the retrieved wavefont as compared with current Shack-Hartmann wavefront sensors. Here we demonstrate effective correction of the PSF in 3 iterations. Starting from a severely aberrated system, we achieve a Strehl ratio of 0.78 and a greater than 10-fold increase in maximum intensity. |
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| معلومات مُعتمدة: | R01 GM025101 United States GM NIGMS NIH HHS; R01 GM031627 United States GM NIGMS NIH HHS |
| فهرسة مساهمة: | Keywords: Adaptive Optics; Biomedical Imaging; Microscopy |
| تواريخ الأحداث: | Date Created: 20140107 Latest Revision: 20211021 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC3877333 |
| DOI: | 10.1117/12.840943 |
| PMID: | 24392198 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0277-786X |
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| DOI: | 10.1117/12.840943 |