دورية أكاديمية
Circulating but not immobilized N-deglycosylated von Willebrand factor increases platelet adhesion under flow conditions.
العنوان: | Circulating but not immobilized N-deglycosylated von Willebrand factor increases platelet adhesion under flow conditions. |
---|---|
المؤلفون: | Fallah MA; University of Augsburg, Chair of Experimental Physics I, 86159 Augsburg, Germany ; Department of Biophysical Chemistry, University of Konstanz, 78457 Konstanz, Germany., Huck V; Heidelberg University, Medical Faculty Mannheim, Experimental Dermatology, 68167 Mannheim, Germany., Niemeyer V; Heidelberg University, Medical Faculty Mannheim, Experimental Dermatology, 68167 Mannheim, Germany., Desch A; Heidelberg University, Medical Faculty Mannheim, Experimental Dermatology, 68167 Mannheim, Germany., Angerer JI; University of Augsburg, Chair of Experimental Physics I, 86159 Augsburg, Germany., McKinnon TA; Imperial College London, Hammersmith Hospital Campus, Department of Medicine, London W12 0NN, United Kingdom., Wixforth A; University of Augsburg, Chair of Experimental Physics I, 86159 Augsburg, Germany., Schneider SW; Heidelberg University, Medical Faculty Mannheim, Experimental Dermatology, 68167 Mannheim, Germany., Schneider MF; Department of Mechanical Engineering, Boston University, Boston, Massachusetts 02215, USA. |
المصدر: | Biomicrofluidics [Biomicrofluidics] 2013 Aug 26; Vol. 7 (4), pp. 44124. Date of Electronic Publication: 2013 Aug 26 (Print Publication: 2013). |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: American Institute of Physics Country of Publication: United States NLM ID: 101293825 Publication Model: eCollection Cited Medium: Print ISSN: 1932-1058 (Print) Linking ISSN: 19321058 NLM ISO Abbreviation: Biomicrofluidics Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: Melville, NY : American Institute of Physics, 2007- |
مستخلص: | The role of von Willebrand factor (VWF) as a shear stress activated platelet adhesive has been related to a coiled-elongated shape conformation. The forces dominating this transition have been suggested to be controlled by the proteins polymeric architecture. However, the fact that 20% of VWF molecular weight originates from glycan moieties has so far been neglected in these calculations. In this study, we present a systematic experimental investigation on the role of N-glycosylation for VWF mediated platelet adhesion under flow. A microfluidic flow chamber with a stenotic compartment that allows one to mimic various physiological flow conditions was designed for the efficient analysis of the adhesion spectrum. Surprisingly, we found an increase in platelet adhesion with elevated shear rate, both qualitatively and quantitatively fully conserved when N-deglycosylated VWF (N-deg-VWF) instead of VWF was immobilized in the microfluidic channel. This has been demonstrated consistently over four orders of magnitude in shear rate. In contrast, when N-deg-VWF was added to the supernatant, an increase in adhesion rate by a factor of two was detected compared to the addition of wild-type VWF. It appears that once immobilized, the role of glycans is at least modified if not-as found here for the case of adhesion-negated. These findings strengthen the physical impact of the circulating polymer on shear dependent platelet adhesion events. At present, there is no theoretical explanation for an increase in platelet adhesion to VWF in the absence of its N-glycans. However, our data indicate that the effective solubility of the protein and hence its shape or conformation may be altered by the degree of glycosylation and is therefore a good candidate for modifying the forces required to uncoil this biopolymer. |
References: | J Appl Physiol. 1963 Mar;18:255-60. (PMID: 13935041) Annu Rev Biochem. 1998;67:395-424. (PMID: 9759493) Thromb Res. 2007;120 Suppl 1:S5-9. (PMID: 17493665) J Thromb Haemost. 2011 Feb;9(2):275-81. (PMID: 21054779) Cell. 1998 Sep 4;94(5):657-66. (PMID: 9741630) J Biol Chem. 2011 Oct 21;286(42):36361-7. (PMID: 21896483) Blood. 2006 Sep 15;108(6):1903-10. (PMID: 16772609) Proc Natl Acad Sci U S A. 2008 Jun 17;105(24):8256-61. (PMID: 18550810) Biomicrofluidics. 2013 Apr 05;7(2):21501. (PMID: 24404005) Methods Enzymol. 1994;230:44-57. (PMID: 8139511) J Biomech. 1977;10(5-6):339-53. (PMID: 893472) Nature. 1979 Jun 14;279(5714):636-8. (PMID: 313016) J Biol Chem. 1992 May 5;267(13):8723-31. (PMID: 1577715) Proc Natl Acad Sci U S A. 2007 May 8;104(19):7899-903. (PMID: 17470810) Circulation. 2004 Apr 27;109(16):1930-2. (PMID: 15078797) Ann Biomed Eng. 2011 Feb;39(2):922-9. (PMID: 20949319) Ann Biomed Eng. 2011 May;39(5):1403-13. (PMID: 21203843) Biochemistry. 1985 Aug 13;24(17):4665-71. (PMID: 4063349) Biomicrofluidics. 2011 Sep;5(3):34122-341228. (PMID: 22662047) Arterioscler Thromb Vasc Biol. 1998 Sep;18(9):1359-62. (PMID: 9743222) Scand Cardiovasc J. 2009;43(5):298-303. (PMID: 19263268) Nat Med. 2002 Nov;8(11):1227-34. (PMID: 12411949) Biochemistry. 1986 Jun 3;25(11):3171-84. (PMID: 3524673) Annu Rev Med. 1986;37:157-63. (PMID: 2939788) Thromb Res. 1982 Jan 1-15;25(1-2):81-9. (PMID: 6801813) Blood Rev. 2006 Mar;20(2):83-92. (PMID: 16507387) Phys Rev Lett. 2006 Sep 29;97(13):138101. (PMID: 17026077) Thromb Haemost. 2007 Apr;97(4):514-26. (PMID: 17393012) Biophys J. 2009 Mar 18;96(6):2313-20. (PMID: 19289057) Am J Physiol Heart Circ Physiol. 2009 Dec;297(6):H2128-35. (PMID: 19820200) Semin Hematol. 2005 Jan;42(1):5-14. (PMID: 15662611) Blood. 2008 Mar 15;111(6):3042-9. (PMID: 17975018) J Biol Chem. 1997 Oct 24;272(43):27058-64. (PMID: 9341145) Am J Physiol. 1988 Jun;254(6 Pt 2):H1059-64. (PMID: 3381893) Br Med J. 1964 Jul 18;2(5402):177. (PMID: 14150898) Arterioscler Thromb Vasc Biol. 1997 May;17(5):919-24. (PMID: 9157956) Thromb Res. 1983 Dec 15;32(6):545-56. (PMID: 6420928) Best Pract Res Clin Haematol. 2001 Jun;14(2):257-79. (PMID: 11686099) |
تواريخ الأحداث: | Date Created: 20140110 Date Completed: 20140109 Latest Revision: 20211021 |
رمز التحديث: | 20221213 |
مُعرف محوري في PubMed: | PMC3772935 |
DOI: | 10.1063/1.4819746 |
PMID: | 24404057 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1932-1058 |
---|---|
DOI: | 10.1063/1.4819746 |