دورية أكاديمية
The nucleosome assembly protein TSPYL2 regulates the expression of NMDA receptor subunits GluN2A and GluN2B.
العنوان: | The nucleosome assembly protein TSPYL2 regulates the expression of NMDA receptor subunits GluN2A and GluN2B. |
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المؤلفون: | Tsang KH; 1] Department of Paediatrics and Adolescent Medicine, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong, China [2] Centre for Reproduction, Development and Growth, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong, China., Lai SK; 1] Department of Physiology, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong, China [2] Research Centre of Heart, Brain, Hormone and Healthy Aging, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong, China., Li Q; 1] Centre for Reproduction, Development and Growth, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong, China [2] Department of Psychiatry, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong, China., Yung WH; School of Biomedical Sciences, the Chinese University of Hong Kong, Hong Kong, China., Liu H; 1] Department of Paediatrics and Adolescent Medicine, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong, China [2] Centre for Reproduction, Development and Growth, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong, China., Mak PH; 1] Department of Paediatrics and Adolescent Medicine, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong, China [2] Centre for Reproduction, Development and Growth, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong, China., Ng CC; 1] Department of Paediatrics and Adolescent Medicine, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong, China [2] Centre for Reproduction, Development and Growth, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong, China., McAlonan G; 1] Centre for Reproduction, Development and Growth, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong, China [2] Department of Psychiatry, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong, China [3] Department of Forensic and Neurodevelopmental Sciences, Institute of Psychiatry, King's College London, United Kingdom., Chan YS; 1] Department of Physiology, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong, China [2] Research Centre of Heart, Brain, Hormone and Healthy Aging, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong, China., Chan SY; 1] Department of Paediatrics and Adolescent Medicine, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong, China [2] Centre for Reproduction, Development and Growth, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong, China. |
المصدر: | Scientific reports [Sci Rep] 2014 Jan 13; Vol. 4, pp. 3654. Date of Electronic Publication: 2014 Jan 13. |
نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: London : Nature Publishing Group, copyright 2011- |
مواضيع طبية MeSH: | Gene Expression Regulation*, Nuclear Proteins/*metabolism , Receptors, N-Methyl-D-Aspartate/*genetics, Animals ; DNA-Binding Proteins ; Fear ; Female ; Hippocampus/metabolism ; Learning ; Long-Term Potentiation/genetics ; Male ; Memory ; Mice ; Mutation ; Nuclear Proteins/genetics ; Pyramidal Cells/metabolism ; Receptors, N-Methyl-D-Aspartate/metabolism ; Transcriptional Activation |
مستخلص: | TSPYL2 is an X-linked gene encoding a nucleosome assembly protein. TSPYL2 interacts with calmodulin-associated serine/threonine kinase, which is implicated in X-linked mental retardation. As nucleosome assembly and chromatin remodeling are important in transcriptional regulation and neuronal function, we addressed the importance of TSPYL2 through analyzing Tspyl2 loss-of-function mice. We detected down-regulation of N-methyl-D-aspartate receptor subunits 2A and 2B (GluN2A and GluN2B) in the mutant hippocampus. Evidence from luciferase reporter assays and chromatin immunoprecipitation supported that TSPYL2 regulated the expression of Grin2a and Grin2b, the genes encoding GluN2A and GluN2B. We also detected an interaction between TSPYL2 and CBP, indicating that TSPYL2 may activate gene expression through binding CBP. In terms of functional outcome, Tspyl2 loss-of-function impaired long-term potentiation at hippocampal Schaffer collateral-CA1 synapses. Moreover, mutant mice showed a deficit in fear learning and memory. We conclude that TSPYL2 contributes to cognitive variability through regulating the expression of Grin2a and Grin2b. |
References: | J Biol Chem. 2002 Nov 29;277(48):46374-84. (PMID: 12356765) Mol Cell Biol. 2004 Mar;24(5):2153-68. (PMID: 14966293) Cell. 2001 Jan 12;104(1):119-30. (PMID: 11163245) Nat Neurosci. 2002 Sep;5(9):868-73. (PMID: 12195431) Neuron. 2004 Jun 24;42(6):961-72. (PMID: 15207240) Nat Genet. 2009 May;41(5):535-43. (PMID: 19377476) Nat Genet. 2011 Jun;43(6):585-9. (PMID: 21572417) Mol Cell Biol. 2000 Dec;20(23):8933-43. (PMID: 11073993) Nature. 1995 Jul 27;376(6538):348-51. (PMID: 7630403) Proc Natl Acad Sci U S A. 2005 Jun 28;102(26):9341-6. (PMID: 15961543) Neuron. 2004 Jun 24;42(6):947-59. (PMID: 15207239) Methods Mol Biol. 2004;284:129-46. (PMID: 15173613) Neuron. 2004 Apr 8;42(1):113-28. (PMID: 15066269) J Biol Chem. 2005 Aug 19;280(33):29728-42. (PMID: 15951563) Mol Cell Biol. 2007 Sep;27(17):6093-102. (PMID: 17591696) Genomics. 2002 Apr;79(4):560-72. (PMID: 11944989) Behav Neurosci. 1992 Apr;106(2):274-85. (PMID: 1590953) Psychiatry Res. 1997 May 30;70(3):131-43. (PMID: 9211575) Mol Psychiatry. 2011 Aug;16(8):867-80. (PMID: 20479760) Learn Mem. 2006 Sep-Oct;13(5):609-17. (PMID: 16980541) Learn Mem. 2011 Feb 23;18(3):161-9. (PMID: 21345974) Biochim Biophys Acta. 2002 Dec 12;1588(3):241-6. (PMID: 12393179) Nature. 1993 Jan 7;361(6407):31-9. (PMID: 8421494) Neuron. 1997 Oct;19(4):755-9. (PMID: 9354323) Am J Med Genet A. 2003 Apr 30;118A(3):217-22. (PMID: 12673650) Proc Natl Acad Sci U S A. 2008 Jun 10;105(23):7959-63. (PMID: 18523016) Proc Natl Acad Sci U S A. 2006 Mar 7;103(10):3693-7. (PMID: 16484369) PLoS One. 2011;6(6):e21602. (PMID: 21738728) J Neurosci. 2010 Mar 31;30(13):4590-600. (PMID: 20357110) PLoS One. 2011;6(5):e20507. (PMID: 21655223) Mol Psychiatry. 2007 Oct;12(10):897-9. (PMID: 17895924) Nat Genet. 2010 Nov;42(11):1021-6. (PMID: 20890276) Epigenetics. 2010 Jan 1;5(1):9-15. (PMID: 20110770) Nat Rev Neurosci. 2008 Jan;9(1):65-75. (PMID: 18094707) J Neurosci. 2001 Sep 1;21(17):6889-96. (PMID: 11517276) Psychiatry Res. 2012 Nov 30;204(2-3):178-83. (PMID: 23137804) Biochem Biophys Res Commun. 2005 Sep 23;335(2):322-7. (PMID: 16061203) Proc Natl Acad Sci U S A. 2006 Mar 14;103(11):4270-4. (PMID: 16537520) Nature. 1995 Jan 12;373(6510):151-5. (PMID: 7816096) Cell. 2011 May 27;145(5):787-99. (PMID: 21620140) Biochem Biophys Res Commun. 2010 Sep 24;400(3):419-25. (PMID: 20800572) Psychopharmacology (Berl). 2006 Nov;188(4):552-66. (PMID: 16676163) Neurobiol Dis. 2010 Oct;40(1):155-62. (PMID: 20553872) Genetics. 2006 Aug;173(4):2407-10. (PMID: 16751659) Eur J Hum Genet. 2010 May;18(5):544-52. (PMID: 20029458) Genetics. 2004 Aug;167(4):1821-32. (PMID: 15342520) Curr Biol. 2013 Jan 21;23(2):99-106. (PMID: 23246402) J Comp Neurol. 2006 Feb 1;494(4):606-19. (PMID: 16374801) Nat Rev Genet. 2002 Feb;3(2):114-23. (PMID: 11836505) Neurosignals. 2011;19(3):128-41. (PMID: 21576927) Mol Cell Biol. 2002 May;22(9):2974-83. (PMID: 11940655) Biochem Cell Biol. 2006 Aug;84(4):549-58. (PMID: 16936827) J Neurosci. 2003 May 1;23(9):3953-9. (PMID: 12736365) |
معلومات مُعتمدة: | United Kingdom Medical Research Council |
المشرفين على المادة: | 0 (DNA-Binding Proteins) 0 (NR2B NMDA receptor) 0 (Nuclear Proteins) 0 (Receptors, N-Methyl-D-Aspartate) 0 (TSPYL2 protein, human) VH92ICR8HX (N-methyl D-aspartate receptor subtype 2A) |
تواريخ الأحداث: | Date Created: 20140114 Date Completed: 20150730 Latest Revision: 20211203 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC3888966 |
DOI: | 10.1038/srep03654 |
PMID: | 24413569 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2045-2322 |
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DOI: | 10.1038/srep03654 |