دورية أكاديمية
أعرض في EDS

Pharmacological characterisation of ligand- and voltage-gated ion channels expressed in human iPSC-derived forebrain neurons.

التفاصيل البيبلوغرافية
العنوان: Pharmacological characterisation of ligand- and voltage-gated ion channels expressed in human iPSC-derived forebrain neurons.
المؤلفون: Dage JL; Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN, 46285, USA., Colvin EM, Fouillet A, Langron E, Roell WC, Li J, Mathur SX, Mogg AJ, Schmitt MG, Felder CC, Merchant KM, Isaac J, Broad LM, Sher E, Ursu D
المصدر: Psychopharmacology [Psychopharmacology (Berl)] 2014 Mar; Vol. 231 (6), pp. 1105-24. Date of Electronic Publication: 2014 Jan 16.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 7608025 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-2072 (Electronic) Linking ISSN: 00333158 NLM ISO Abbreviation: Psychopharmacology (Berl) Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Berlin, New York, Springer-Verlag.
مواضيع طبية MeSH: Induced Pluripotent Stem Cells/*physiology , Ion Channels/*metabolism , Neurons/*physiology , Prosencephalon/*physiology, Calcium/metabolism ; Calcium Channels/metabolism ; Gene Expression ; Humans ; Induced Pluripotent Stem Cells/drug effects ; Membrane Potentials/drug effects ; Membrane Potentials/physiology ; Neurons/drug effects ; Prosencephalon/drug effects ; RNA, Messenger/metabolism ; Receptors, GABA/metabolism ; Receptors, GABA-A/metabolism ; Receptors, Ionotropic Glutamate/agonists ; Receptors, Ionotropic Glutamate/metabolism ; Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors ; Receptors, N-Methyl-D-Aspartate/metabolism ; Time Factors ; Voltage-Gated Sodium Channels/metabolism
مستخلص: Introduction: Genetic causes, or predisposition, are increasingly accepted to be part of the ethiopathogenesis of many neuropsychiatric diseases. While genes can be studied in any type of cells, their physiological function in human brain cells is difficult to evaluate, particularly in living subjects.
Methods: As a first step towards the characterisation of human inducible pluripotent stem cell (iPSC)-derived neurons from autism spectrum disorder (ASD) patients, we used gene expression and functional studies to define the regional identity of the typical forebrain differentiation, demonstrate expression patterns of genes of interest in ASD and understand the properties of 'control' iPSC-derived neurons (iCell-Neurons™), with a focus on receptors and ion channels that play a central role in synaptic physio-pathology.
Results and Discussion: The gene expression profile of the iCell-Neurons™ closely resembled that observed in neonatal prefrontal cortex tissues. Functional studies, performed mainly using calcium flux assays, demonstrated the presence of ionotropic glutamate (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid and N-methyl-D-aspartate) and gamma-aminobutyric acid type A receptors. Voltage-gated sodium and calcium channels were also identified using similar techniques.
Conclusions: Overall, the results reported here suggest that iCell-Neurons™ are a good cellular model of a relatively immature forebrain human neuron population that can be used both as a control in comparison to patients cells, and as host cells in which mutations, insertions and deletions can be used in order to study the molecular mechanisms of ASD and other neurological disorders in an isogenic cellular background.
References: J Biomol Screen. 2012 Oct;17(9):1264-72. (PMID: 22923790)
J Neurophysiol. 1993 Oct;70(4):1606-16. (PMID: 8283217)
Mol Pharmacol. 1994 May;45(5):846-53. (PMID: 8190101)
Neuropharmacology. 2013 Mar;66:264-73. (PMID: 22659090)
J Neurosci. 1996 Oct 15;16(20):6414-23. (PMID: 8815920)
Nat Rev Drug Discov. 2012 Nov;11(11):815-6. (PMID: 23123927)
Br J Pharmacol. 2001 Apr;132(8):1859-75. (PMID: 11309259)
Neurochem Int. 2011 Sep;59(3):404-12. (PMID: 21315124)
Nucleic Acids Res. 2012 May;40(9):3785-99. (PMID: 22262733)
CNS Drug Rev. 2002 Fall;8(3):235-54. (PMID: 12353057)
Neuron. 2010 Sep 23;67(6):984-96. (PMID: 20869595)
Genomics. 2012 Dec;100(6):337-44. (PMID: 22959562)
Brain Res Dev Brain Res. 1998 Aug 8;109(2):281-92. (PMID: 9729431)
Mol Psychiatry. 2009 Mar;14(3):252-60. (PMID: 19065143)
Stem Cell Res. 2012 Jan;8(1):134-40. (PMID: 22099027)
Stem Cell Res. 2013 Mar;10(2):213-27. (PMID: 23305945)
Curr Stem Cell Res Ther. 2010 Sep;5(3):251-60. (PMID: 20214556)
Br J Pharmacol. 2012 Jun;166(3):924-37. (PMID: 22022974)
Exp Neurol. 2009 Jul;218(1):109-16. (PMID: 19393237)
JAMA. 2011 Nov 9;306(18):2001-10. (PMID: 22068992)
PLoS One. 2013;8(1):e55047. (PMID: 23383051)
J Neurosci. 1992 Dec;12(12):4834-45. (PMID: 1361198)
Biostatistics. 2003 Apr;4(2):249-64. (PMID: 12925520)
J Pharmacol Exp Ther. 2010 Dec;335(3):636-44. (PMID: 20810618)
Nat Med. 2005 Nov;11(11):1205-13. (PMID: 16227993)
Nucleic Acids Res. 2005 Nov 10;33(20):e175. (PMID: 16284200)
J Neurochem. 1995 Feb;64(2):531-9. (PMID: 7830045)
Nat Biotechnol. 2008 Nov;26(11):1276-84. (PMID: 18931654)
Nat Neurosci. 2013 Feb;16(2):201-9. (PMID: 23313911)
Neuron. 2007 Sep 6;55(5):779-85. (PMID: 17785184)
Neurosci Lett. 2012 May 10;516(1):9-14. (PMID: 22405972)
Neuropharmacology. 2011 Jun;60(7-8):1355-63. (PMID: 21371482)
Bioinformatics. 2009 Feb 1;25(3):415-6. (PMID: 19106121)
Mol Psychiatry. 2010 Feb;15(2):119-21. (PMID: 20098439)
J Pharmacol Exp Ther. 2001 Jul;298(1):86-102. (PMID: 11408529)
Neurobiol Dis. 2012 Dec;48(3):356-66. (PMID: 22668776)
Nucleic Acids Res. 2013 Jan;41(Database issue):D991-5. (PMID: 23193258)
Trends Pharmacol Sci. 2011 Dec;32(12):726-33. (PMID: 21996280)
Neuropharmacology. 2012 Sep;63(3):441-9. (PMID: 22579927)
Assay Drug Dev Technol. 2008 Apr;6(2):195-212. (PMID: 18471074)
Neuron. 1991 Mar;6(3):333-44. (PMID: 1672071)
Proc Natl Acad Sci U S A. 2011 Dec 13;108(50):20189-94. (PMID: 22106298)
Eur J Neurosci. 2005 May;21(9):2593-9. (PMID: 15932617)
Genome Biol. 2004;5(10):R80. (PMID: 15461798)
Stem Cells Transl Med. 2012 Jan;1(1):36-43. (PMID: 23197638)
Dev Cogn Neurosci. 2011 Jan;1(1):47-56. (PMID: 21151713)
Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15545-50. (PMID: 16199517)
J Biol Chem. 2012 May 18;287(21):17438-17446. (PMID: 22474321)
Cell Stem Cell. 2008 Sep 11;3(3):340-5. (PMID: 18786420)
Pediatrics. 2004 May;113(5):e472-86. (PMID: 15121991)
Toxicol Sci. 2012 Apr;126(2):426-35. (PMID: 22223483)
Nature. 2009 May 28;459(7246):528-33. (PMID: 19404256)
Br J Clin Pharmacol. 2013 Apr;75(4):907-18. (PMID: 23126226)
Cell. 2011 Jun 10;145(6):831-4. (PMID: 21663789)
Nucleic Acids Res. 2009 Jan;37(Database issue):D832-6. (PMID: 19015121)
Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):12770-5. (PMID: 22761314)
PLoS Genet. 2007 Sep;3(9):1724-35. (PMID: 17907809)
Cell. 2008 Sep 5;134(5):877-86. (PMID: 18691744)
Cell. 2010 Nov 12;143(4):527-39. (PMID: 21074045)
J Neurophysiol. 1991 Feb;65(2):247-63. (PMID: 1673153)
Nat Rev Drug Discov. 2011 Nov 11;10(12):915-29. (PMID: 22076509)
Behav Brain Res. 2008 Mar 5;187(2):207-20. (PMID: 17983671)
Eur J Pharmacol. 2002 Mar 1;438(1-2):53-62. (PMID: 11906710)
BMC Genomics. 2006 Oct 09;7:252. (PMID: 17029630)
Curr Neurol Neurosci Rep. 2009 May;9(3):188-97. (PMID: 19348707)
Curr Opin Pharmacol. 2007 Feb;7(1):39-47. (PMID: 17088105)
Nat Med. 2011 Nov 27;17(12):1657-62. (PMID: 22120178)
Sci Transl Med. 2012 Aug 1;4(145):145ra104. (PMID: 22855461)
Nat Methods. 2009 May;6(5):370-6. (PMID: 19404254)
المشرفين على المادة: 0 (Calcium Channels)
0 (Ion Channels)
0 (RNA, Messenger)
0 (Receptors, GABA)
0 (Receptors, GABA-A)
0 (Receptors, Ionotropic Glutamate)
0 (Receptors, N-Methyl-D-Aspartate)
0 (Voltage-Gated Sodium Channels)
SY7Q814VUP (Calcium)
تواريخ الأحداث: Date Created: 20140117 Date Completed: 20141112 Latest Revision: 20220309
رمز التحديث: 20240628
DOI: 10.1007/s00213-013-3384-2
PMID: 24429870
قاعدة البيانات: MEDLINE
الوصف
تدمد:1432-2072
DOI:10.1007/s00213-013-3384-2