دورية أكاديمية
Imaging of adipose tissue.
| العنوان: | Imaging of adipose tissue. |
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| المؤلفون: | Berry R; Department of Molecular, Cell, and Developmental Biology, Yale University School of Medicine, New Haven, Connecticut, USA., Church CD; Section of Comparative Medicine, Yale University School of Medicine, New Haven, Connecticut, USA., Gericke MT; Institute of Anatomy, University of Leipzig, Leipzig, Germany., Jeffery E; Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut, USA., Colman L; Section of Comparative Medicine, Yale University School of Medicine, New Haven, Connecticut, USA., Rodeheffer MS; Department of Molecular, Cell, and Developmental Biology, Yale University School of Medicine, New Haven, Connecticut, USA; Section of Comparative Medicine, Yale University School of Medicine, New Haven, Connecticut, USA; Yale Stem Cell Center, Yale University School of Medicine, New Haven, Connecticut, USA. Electronic address: matthew.rodeheffer@yale.edu. |
| المصدر: | Methods in enzymology [Methods Enzymol] 2014; Vol. 537, pp. 47-73. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0212271 Publication Model: Print Cited Medium: Internet ISSN: 1557-7988 (Electronic) Linking ISSN: 00766879 NLM ISO Abbreviation: Methods Enzymol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | Adipose Tissue, Brown/*ultrastructure , Adipose Tissue, White/*ultrastructure , Microscopy, Confocal/*methods , Obesity/*pathology, Adiposity/genetics ; Diagnostic Imaging ; Humans ; Lipid Metabolism ; Obesity/diagnosis ; Obesity/genetics ; Thermogenesis |
| مستخلص: | Adipose tissue is an endocrine organ that specializes in lipid metabolism and is distributed throughout the body in distinct white adipose tissue (WAT) and brown adipose tissue (BAT) depots. These tissues have opposing roles in lipid metabolism with WAT storing excessive caloric intake in the form of lipid, and BAT burning lipid through nonshivering thermogenesis. As accumulation of lipid in mature adipocytes of WAT leads to obesity and increased risk of comorbidity (Pi-Sunyer et al., 1998), detailed understanding of the mechanisms of BAT activation and WAT accumulation could produce therapeutic strategies for combatting metabolic pathologies. As morphological changes accompany alterations in adipose function, imaging of adipose tissue is one of the most important tools for understanding how adipose tissue mass fluctuates in response to various physiological contexts. Therefore, this chapter details several methods of processing and imaging adipose tissue, including bright-field colorimetric imaging of paraffin-sectioned adipose tissue with a detailed protocol for automated adipocyte size analysis; fluorescent imaging of paraffin and frozen-sectioned adipose tissue; and confocal fluorescent microscopy of whole mounted adipose tissue. We have also provided many example images showing results produced using each protocol, as well as commentary on the strengths and limitations of each approach. (© 2014 Elsevier Inc. All rights reserved.) |
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| معلومات مُعتمدة: | T32 GM007223 United States GM NIGMS NIH HHS; DK090489 United States DK NIDDK NIH HHS; R56 DK090489 United States DK NIDDK NIH HHS; P30 DK045735 United States DK NIDDK NIH HHS; R01 DK090489 United States DK NIDDK NIH HHS; DK045735 United States DK NIDDK NIH HHS |
| فهرسة مساهمة: | Keywords: Adipose; Cell profiler; Confocal; Frozen; Lineage tracing; Paraffin; Whole mount |
| تواريخ الأحداث: | Date Created: 20140201 Date Completed: 20140919 Latest Revision: 20211021 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC4272855 |
| DOI: | 10.1016/B978-0-12-411619-1.00004-5 |
| PMID: | 24480341 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1557-7988 |
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| DOI: | 10.1016/B978-0-12-411619-1.00004-5 |