دورية أكاديمية
DNA repair signalling pathway genes are overexpressed in poor-quality pre-implantation human embryos with complex aneuploidy.
| العنوان: | DNA repair signalling pathway genes are overexpressed in poor-quality pre-implantation human embryos with complex aneuploidy. |
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| المؤلفون: | Bazrgar M; Department of Genetics at Reproductive Biomedicine Research Centre, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran; Department of Developmental Biology, University of Science and Culture, Tehran, Iran., Gourabi H; Department of Genetics at Reproductive Biomedicine Research Centre, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. Electronic address: gourabi@royaninstitute.org., Yazdi PE; Department of Embryology at Reproductive Biomedicine Research Centre, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran., Vazirinasab H; Department of Genetics at Reproductive Biomedicine Research Centre, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran., Fakhri M; Department of Genetics at Reproductive Biomedicine Research Centre, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran., Hassani F; Department of Embryology at Reproductive Biomedicine Research Centre, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran., Valojerdi MR; Department of Embryology at Reproductive Biomedicine Research Centre, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. |
| المصدر: | European journal of obstetrics, gynecology, and reproductive biology [Eur J Obstet Gynecol Reprod Biol] 2014 Apr; Vol. 175, pp. 152-6. Date of Electronic Publication: 2014 Jan 15. |
| نوع المنشور: | Comparative Study; Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Scientific Publishers Country of Publication: Ireland NLM ID: 0375672 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7654 (Electronic) Linking ISSN: 03012115 NLM ISO Abbreviation: Eur J Obstet Gynecol Reprod Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Limerick : Elsevier Scientific Publishers Original Publication: Amsterdam, Excerpta Medica. |
| مواضيع طبية MeSH: | Aneuploidy* , Gene Expression Regulation, Developmental*, DNA Repair/*genetics , Embryo, Mammalian/*metabolism, Apoptosis/genetics ; Cell Cycle/genetics ; Embryo Research ; Humans ; Real-Time Polymerase Chain Reaction ; Signal Transduction/genetics |
| مستخلص: | Objective: Chromosomal abnormalities and poor quality are correlated with DNA damage in the pre-implantation stage in humans. This study aimed to explore the altered expression of DNA damage signalling pathways - including apoptosis, cell cycle and DNA repair pathways - in poor-quality pre-implantation human embryos with complex aneuploidy. Study Design: Surplus Day 4 embryos from candidates undergoing pre-implantation genetic screening were pooled into two groups. Group 1 included good-quality embryos that had simple aneuploidy, a single chromosome [according to fluorescence in situ hybridization-based pre-implantation genetic diagnosis (PGD) on Day 3], a normal rate of cell division, and graded as A or B (excellent to good). Group 2 included embryos with more than one aneuploid chromosome on PGD on Day 3, an abnormal rate of cell division, and graded as C or D (fair to poor). Gene expression of DNA damage signalling pathways was analysed using a real-time polymerase chain reaction-based array, which included 84 genes after specific pre-amplification of cDNA by a primer mix, including all array genes. Results: In Group 2, five of the 84 genes studied showed significant overexpression (p<0.05): MSH3, XRCC1, RAD50, LIG1 and CDK7. Alterations were in agreement with genetic relationships in pathway analyses on DAVID. Conclusions: The five overexpressed genes are involved in DNA repair. Therefore, in comparison with cell cycle control and apoptotic pathways, DNA repair pathways are more activated in poor-quality pre-implantation human embryos with complex aneuploidy. This suggests that the dominant response to DNA damage in such embryos is DNA repair rather than cell division or apoptosis. (Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Aneuploidy; DNA damage; DNA repair; Embryo quality; Gene expression; Pre-implantation |
| تواريخ الأحداث: | Date Created: 20140204 Date Completed: 20141217 Latest Revision: 20140407 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.ejogrb.2014.01.010 |
| PMID: | 24485984 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1872-7654 |
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| DOI: | 10.1016/j.ejogrb.2014.01.010 |