دورية أكاديمية

Expression Analysis of Sugarcane Aquaporin Genes under Water Deficit.

التفاصيل البيبلوغرافية
العنوان: Expression Analysis of Sugarcane Aquaporin Genes under Water Deficit.
المؤلفون: da Silva MD; Department of Genetics, Federal University of Pernambuco (UFPE), 50670-901 Recife, PE, Brazil., Silva RL; Department of Genetics, Federal University of Pernambuco (UFPE), 50670-901 Recife, PE, Brazil., Costa Ferreira Neto JR; Department of Genetics, Federal University of Pernambuco (UFPE), 50670-901 Recife, PE, Brazil., Guimarães AC; Biotechnology Division, Sugarcane Technology Center (CTC), 13400-970 Piracicaba, SP, Brazil., Veiga DT; Biotechnology Division, Sugarcane Technology Center (CTC), 13400-970 Piracicaba, SP, Brazil., Chabregas SM; Biotechnology Division, Sugarcane Technology Center (CTC), 13400-970 Piracicaba, SP, Brazil., Burnquist WL; Biotechnology Division, Sugarcane Technology Center (CTC), 13400-970 Piracicaba, SP, Brazil., Kahl G; Institute of Molecular Biosciences, Frankfurt University, 60438 Frankfurt am Main, Germany., Benko-Iseppon AM; Department of Genetics, Federal University of Pernambuco (UFPE), 50670-901 Recife, PE, Brazil., Kido EA; Department of Genetics, Federal University of Pernambuco (UFPE), 50670-901 Recife, PE, Brazil.
المصدر: Journal of nucleic acids [J Nucleic Acids] 2013; Vol. 2013, pp. 763945. Date of Electronic Publication: 2013 Dec 29.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Hindawi Country of Publication: United States NLM ID: 101533042 Publication Model: Print-Electronic Cited Medium: Print ISSN: 2090-0201 (Print) Linking ISSN: 20900201 NLM ISO Abbreviation: J Nucleic Acids Subsets: PubMed not MEDLINE
أسماء مطبوعة: Publication: 2011- : New York : Hindawi
Original Publication: London : SAGE-Hindawi Access to Research
مستخلص: The present work is a pioneer study specifically addressing the aquaporin transcripts in sugarcane transcriptomes. Representatives of the four aquaporin subfamilies (PIP, TIP, SIP, and NIP), already described for higher plants, were identified. Forty-two distinct aquaporin isoforms were expressed in four HT-SuperSAGE libraries from sugarcane roots of drought-tolerant and -sensitive genotypes, respectively. At least 10 different potential aquaporin isoform targets and their respective unitags were considered to be promising for future studies and especially for the development of molecular markers for plant breeding. From those 10 isoforms, four (SoPIP2-4, SoPIP2-6, OsPIP2-4, and SsPIP1-1) showed distinct responses towards drought, with divergent expressions between the bulks from tolerant and sensitive genotypes, when they were compared under normal and stress conditions. Two targets (SsPIP1-1 and SoPIP1-3/PIP1-4) were selected for validation via RT-qPCR and their expression patterns as detected by HT-SuperSAGE were confirmed. The employed validation strategy revealed that different genotypes share the same tolerant or sensitive phenotype, respectively, but may use different routes for stress acclimation, indicating the aquaporin transcription in sugarcane to be potentially genotype-specific.
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تواريخ الأحداث: Date Created: 20140204 Date Completed: 20140203 Latest Revision: 20211021
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC3893750
DOI: 10.1155/2013/763945
PMID: 24490055
قاعدة البيانات: MEDLINE
الوصف
تدمد:2090-0201
DOI:10.1155/2013/763945