دورية أكاديمية
Determination of venlafaxine and O-desmethylvenlafaxine in dried blood spots for TDM purposes, using LC-MS/MS.
| العنوان: | Determination of venlafaxine and O-desmethylvenlafaxine in dried blood spots for TDM purposes, using LC-MS/MS. |
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| المؤلفون: | Berm EJ; Department of Pharmacy, Section Pharmacotherapy and Pharmaceutical Care, University of Groningen, Antonius Deusinglaan 1, Groningen, 9713AV, The Netherlands, e.j.j.berm@rug.nl., Brummel-Mulder E, Paardekooper J, Hak E, Wilffert B, Maring JG |
| المصدر: | Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2014 Apr; Vol. 406 (9-10), pp. 2349-53. Date of Electronic Publication: 2014 Feb 04. |
| نوع المنشور: | Evaluation Study; Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 101134327 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-2650 (Electronic) Linking ISSN: 16182642 NLM ISO Abbreviation: Anal Bioanal Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Heidelberg : Springer-Verlag, 2002- |
| مواضيع طبية MeSH: | Antidepressive Agents/*blood , Chromatography, High Pressure Liquid/*methods , Cyclohexanols/*blood , Dried Blood Spot Testing/*methods , Drug Monitoring/*methods , Tandem Mass Spectrometry/*methods, Desvenlafaxine Succinate ; Humans ; Sensitivity and Specificity ; Venlafaxine Hydrochloride |
| مستخلص: | Dried blood spot (DBS) sampling and quantitative analyses of many current therapeutic drug monitoring (TDM)-guided drugs are advantageous because of the minimal invasive sampling strategy. Here, a fast and robust LC-MS/MS method was developed and analytically validated for simultaneous determination of venlafaxine (VEN) and O-desmethylvenlafaxine (ODV) in DBS. Six-millimeter circles were punched out from DBS collected on Whatman DMPK-C paper, and the DBS was extracted with acetonitrile/methanol at 1:3. The total run time was 4.8 min. The assay was linear in the range of 20-1,000 μg/L for both VEN and ODV. Assay accuracy and precision was well within limits of acceptance (LLOQ = 20 μg/L). Normal hematocrit concentrations (0.30-0.50) did not influence the results neither did a normal spot volume (40-80 μL). Punch position at the perimeter instead of the center of the blood spot gave a bias ranging from 2.4 to 10.4%. Correlation between plasma and spiked DBS samples was high. The concentrations found in spiked DBS samples were higher than those in plasma, indicating that a conversion factor for translation of DBS to plasma values is needed. This analytically validated method is suitable for determination of VEN and ODV in DBS and applicable for TDM. The method will be used for TDM of VEN in the Dutch CYSCE multicenter trial (NCT01778907). |
| سلسلة جزيئية: | ClinicalTrials.gov NCT01778907 |
| المشرفين على المادة: | 0 (Antidepressive Agents) 0 (Cyclohexanols) 7D7RX5A8MO (Venlafaxine Hydrochloride) ZB22ENF0XR (Desvenlafaxine Succinate) |
| تواريخ الأحداث: | Date Created: 20140205 Date Completed: 20150416 Latest Revision: 20191210 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1007/s00216-014-7619-9 |
| PMID: | 24493333 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1618-2650 |
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| DOI: | 10.1007/s00216-014-7619-9 |