دورية أكاديمية
[Activation of ganglioside GM3 biosynthesis in human blood mononuclear cells in atherosclerosis].
| العنوان: | [Activation of ganglioside GM3 biosynthesis in human blood mononuclear cells in atherosclerosis]. |
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| المؤلفون: | Gracheva EV, Samovilova NN, Piksina GF, Shishkina VS, Prokazova NV |
| المصدر: | Biomeditsinskaia khimiia [Biomed Khim] 2013 Jul-Aug; Vol. 59 (4), pp. 459-68. |
| نوع المنشور: | Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | Russian |
| بيانات الدورية: | Publisher: Rossiĭskai︠a︡ akademii︠a︡ medit︠s︡inskikh nauk Country of Publication: Russia (Federation) NLM ID: 101196966 Publication Model: Print Cited Medium: Print ISSN: 2310-6972 (Print) Linking ISSN: 23106905 NLM ISO Abbreviation: Biomed Khim Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Moskva : Rossiĭskai︠a︡ akademii︠a︡ medit︠s︡inskikh nauk, 2003- |
| مواضيع طبية MeSH: | Atherosclerosis/*metabolism , G(M3) Ganglioside/*biosynthesis , Lymphocytes/*metabolism , Monocytes/*metabolism, Atherosclerosis/pathology ; CD11b Antigen/biosynthesis ; Female ; Human Umbilical Vein Endothelial Cells/metabolism ; Human Umbilical Vein Endothelial Cells/pathology ; Humans ; Lipopolysaccharides/toxicity ; Lymphocytes/pathology ; Male ; Monocytes/pathology |
| مستخلص: | Using blood monocytes and lymphocytes from atherosclerotic patients and healthy subjects we have investigated activity of GM3 synthase, cellular levels of ganglioside GM3 and its role in monocyte adhesion to cultured human umbilical vein endothelial cells (HUVEC). The results showed that activity of GM3 synthase and cellular levels of ganglioside GM3 in blood mononuclear cells from atherosclerotic patients were several-fold higher than those from healthy subjects. In monocytes the activity of GM3 synthase was one an order of magnitude higher than in lymphocytes from both groups studied; this suggests the major contribution of monocytes to enhanced biosynthesis and levels of GM3 in mononuclear cells in atherosclerosis. Enrichment of monocytes from healthy subjects with ganglioside GM3 by incubation in medium containing this ganglioside increased adherence of these monocytes to HUVEC up to the values typical for monocytes from atherosclerotic patients. In addition, an increase in CD1 1b integrin expression was observed that was comparable to that seen in lipopolysaccharide-activated monocytes. It is suggested that in atherosclerosis the enhanced cellular levels of GM3 in monocytes and lymphocytes may be an important element of cell activation that facilitates their adhesion to endothelial cells and penetration into intima. |
| المشرفين على المادة: | 0 (CD11b Antigen) 0 (G(M3) Ganglioside) 0 (ITGAM protein, human) 0 (Lipopolysaccharides) |
| تواريخ الأحداث: | Date Created: 20140208 Date Completed: 20140318 Latest Revision: 20191112 |
| رمز التحديث: | 20240628 |
| DOI: | 10.18097/pbmc20135904459 |
| PMID: | 24502144 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2310-6972 |
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| DOI: | 10.18097/pbmc20135904459 |