دورية أكاديمية
Isolation and characterization of potential cancer stem cells from solid human tumors--potential applications.
| العنوان: | Isolation and characterization of potential cancer stem cells from solid human tumors--potential applications. |
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| المؤلفون: | Dobbin ZC; Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, University of Alabama at Birmingham, Birmingham, Alabama., Landen CN; Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, University of Alabama at Birmingham, Birmingham, Alabama. |
| المصدر: | Current protocols in pharmacology [Curr Protoc Pharmacol] 2013 Dec 02; Vol. 63, pp. 14.28.1-14.28.19. Date of Electronic Publication: 2013 Dec 02. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Country of Publication: United States NLM ID: 9717249 Publication Model: Electronic Cited Medium: Internet ISSN: 1934-8290 (Electronic) Linking ISSN: 19348282 NLM ISO Abbreviation: Curr Protoc Pharmacol Subsets: MEDLINE |
| مواضيع طبية MeSH: | Neoplastic Stem Cells/*physiology , Tissue and Organ Harvesting/*methods, Animals ; Biological Assay/methods ; Biomarkers, Tumor/analysis ; Carcinogenicity Tests/methods ; Flow Cytometry/methods ; Heterografts/physiology ; Humans ; Indicators and Reagents/pharmacology ; Magnetic Fields ; Mice ; Neoplastic Stem Cells/cytology |
| مستخلص: | Cancer stem cells (CSCs) are a subpopulation of cells within a heterogeneous tumor that have enhanced biologic properties, e.g., increased capacity for self-renewal, increased tumorigenicity, enhanced differentiation capacity, and resistance to chemo- and radiotherapies. This unit describes protocols to isolate and characterize potential cancer stem cells from a solid tumor. These involve creating a single-cell suspension from tumor tissue, tagging the cell subpopulations of interest, and sorting them into different populations. The sorted subpopulations can be evaluated for their ability to meet the functional requirements of a CSC, which primarily include increased tumorigenicity in an in vivo xenograft assay. Use of the protocols described in this unit makes it possible to study populations of cells that may have properties of CSCs. (Copyright © 2013 John Wiley & Sons, Inc.) |
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| معلومات مُعتمدة: | K12 HD000849 United States HD NICHD NIH HHS; UL1 RR025777 United States RR NCRR NIH HHS; 5UL1RR025777 United States RR NCRR NIH HHS; K12 HD00849 United States HD NICHD NIH HHS |
| فهرسة مساهمة: | Keywords: cancer stem cells; cell separation and sorting; xenograft assay |
| المشرفين على المادة: | 0 (Biomarkers, Tumor) 0 (Indicators and Reagents) |
| تواريخ الأحداث: | Date Created: 20140211 Date Completed: 20140926 Latest Revision: 20230105 |
| رمز التحديث: | 20230105 |
| مُعرف محوري في PubMed: | PMC4041697 |
| DOI: | 10.1002/0471141755.ph1428s63 |
| PMID: | 24510756 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1934-8290 |
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| DOI: | 10.1002/0471141755.ph1428s63 |