دورية أكاديمية
أعرض في EDS

Reprogramming to a pluripotent state modifies mesenchymal stem cell resistance to oxidative stress.

التفاصيل البيبلوغرافية
العنوان: Reprogramming to a pluripotent state modifies mesenchymal stem cell resistance to oxidative stress.
المؤلفون: Asensi KD; Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Fortunato RS, dos Santos DS, Pacheco TS, de Rezende DF, Rodrigues DC, Mesquita FC, Kasai-Brunswick TH, de Carvalho AC, Carvalho DP, Carvalho AB, Goldenberg RC
المصدر: Journal of cellular and molecular medicine [J Cell Mol Med] 2014 May; Vol. 18 (5), pp. 824-31. Date of Electronic Publication: 2014 Feb 14.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Wiley-Blackwell Country of Publication: England NLM ID: 101083777 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1582-4934 (Electronic) Linking ISSN: 15821838 NLM ISO Abbreviation: J Cell Mol Med Subsets: MEDLINE
أسماء مطبوعة: Publication: Oxford, England : Wiley-Blackwell
Original Publication: Bucharest : "Carol Davila" University Press, 2000-
مواضيع طبية MeSH: Cellular Reprogramming* , Oxidative Stress*, Mesenchymal Stem Cells/*cytology , Pluripotent Stem Cells/*cytology, Adult ; Antioxidants/metabolism ; Cell Adhesion ; Cell Differentiation ; Cell Line ; Cell Proliferation ; Female ; Flow Cytometry ; Humans ; Karyotyping ; Menstruation ; Mesoderm/cytology ; Phenotype ; Reactive Oxygen Species/metabolism ; Time Factors
مستخلص: Properties of induced pluripotent stem cells (iPSC) have been extensively studied since their first derivation in 2006. However, the modification in reactive oxygen species (ROS) production and detoxification caused by reprogramming still needs to be further elucidated. The objective of this study was to compare the response of iPSC generated from menstrual blood-derived mesenchymal stem cells (mb-iPSC), embryonic stem cells (H9) and adult menstrual blood-derived mesenchymal stem cells (mbMSC) to ROS exposure and investigate the effects of reprogramming on cellular oxidative stress (OS). mbMSC were extremely resistant to ROS exposure, however, mb-iPSC were 10-fold less resistant to H(2)O(2), which was very similar to embryonic stem cell sensitivity. Extracellular production of ROS was also similar in mb-iPSC and H9 and almost threefold lower than in mbMSC. Furthermore, intracellular amounts of ROS were higher in mb-iPSC and H9 when compared with mbMSC. As the ability to metabolize ROS is related to antioxidant enzymes, we analysed enzyme activities in these cell types. Catalase and superoxide dismutase activities were reduced in mb-iPSC and H9 when compared with mbMSC. Finally, cell adhesion under OS conditions was impaired in mb-iPSC when compared with mbMSC, albeit similar to H9. Thus, reprogramming leads to profound modifications in extracellular ROS production accompanied by loss of the ability to handle OS.
(© 2014 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.)
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فهرسة مساهمة: Keywords: induced pluripotent stem cells; menstrual blood; mesenchymal stem cells; oxidative stress; reactive oxygen species
المشرفين على المادة: 0 (Antioxidants)
0 (Reactive Oxygen Species)
تواريخ الأحداث: Date Created: 20140218 Date Completed: 20150406 Latest Revision: 20211021
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC4119388
DOI: 10.1111/jcmm.12226
PMID: 24528612
قاعدة البيانات: MEDLINE
الوصف
تدمد:1582-4934
DOI:10.1111/jcmm.12226