دورية أكاديمية
Scaffold-mediated lentiviral transduction for functional tissue engineering of cartilage.
العنوان: | Scaffold-mediated lentiviral transduction for functional tissue engineering of cartilage. |
---|---|
المؤلفون: | Brunger JM; Departments of Orthopaedic Surgery and Cell Biology, Duke University Medical Center, Durham, NC 27710., Huynh NP, Guenther CM, Perez-Pinera P, Moutos FT, Sanchez-Adams J, Gersbach CA, Guilak F |
المصدر: | Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2014 Mar 04; Vol. 111 (9), pp. E798-806. Date of Electronic Publication: 2014 Feb 18. |
نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
اللغة: | English |
بيانات الدورية: | Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1091-6490 (Electronic) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Washington, DC : National Academy of Sciences |
مواضيع طبية MeSH: | Cell Differentiation/*physiology , Chondrogenesis/*physiology , Extracellular Matrix/*physiology , Tissue Engineering/*methods , Tissue Scaffolds/*virology , Transduction, Genetic/*methods, Analysis of Variance ; Biomechanical Phenomena ; DNA Primers/genetics ; Flow Cytometry ; Gene Transfer Techniques ; Humans ; Immunohistochemistry ; Lentivirus ; Mesenchymal Stem Cells/metabolism ; Microscopy, Electron, Scanning ; Microscopy, Fluorescence ; Polyesters ; Polylysine ; Regenerative Medicine/methods ; Transforming Growth Factor beta3/genetics |
مستخلص: | The ability to develop tissue constructs with matrix composition and biomechanical properties that promote rapid tissue repair or regeneration remains an enduring challenge in musculoskeletal engineering. Current approaches require extensive cell manipulation ex vivo, using exogenous growth factors to drive tissue-specific differentiation, matrix accumulation, and mechanical properties, thus limiting their potential clinical utility. The ability to induce and maintain differentiation of stem cells in situ could bypass these steps and enhance the success of engineering approaches for tissue regeneration. The goal of this study was to generate a self-contained bioactive scaffold capable of mediating stem cell differentiation and formation of a cartilaginous extracellular matrix (ECM) using a lentivirus-based method. We first showed that poly-L-lysine could immobilize lentivirus to poly(ε-caprolactone) films and facilitate human mesenchymal stem cell (hMSC) transduction. We then demonstrated that scaffold-mediated gene delivery of transforming growth factor β3 (TGF-β3), using a 3D woven poly(ε-caprolactone) scaffold, induced robust cartilaginous ECM formation by hMSCs. Chondrogenesis induced by scaffold-mediated gene delivery was as effective as traditional differentiation protocols involving medium supplementation with TGF-β3, as assessed by gene expression, biochemical, and biomechanical analyses. Using lentiviral vectors immobilized on a biomechanically functional scaffold, we have developed a system to achieve sustained transgene expression and ECM formation by hMSCs. This method opens new avenues in the development of bioactive implants that circumvent the need for ex vivo tissue generation by enabling the long-term goal of in situ tissue engineering. |
References: | Mol Ther. 2002 Apr;5(4):397-404. (PMID: 11945066) In Vivo. 2007 May-Jun;21(3):435-44. (PMID: 17591352) Dev Biol. 1991 May;145(1):99-109. (PMID: 2019328) Biomaterials. 2010 Mar;31(8):2193-200. (PMID: 20034665) Cell Tissue Res. 2011 Jun;344(3):499-509. (PMID: 21503601) J Mech Behav Biomed Mater. 2012 Jul;11:92-101. (PMID: 22658158) J Control Release. 2009 Oct 1;139(1):48-55. (PMID: 19539680) J Virol. 2003 Aug;77(16):8957-61. (PMID: 12885912) J Gene Med. 2008 Oct;10(10):1102-12. (PMID: 18618417) Transl Res. 2013 Apr;161(4):205-16. (PMID: 23369825) Tissue Eng Part A. 2009 Jan;15(1):115-26. (PMID: 18788982) Tissue Eng Part A. 2009 May;15(5):1127-39. (PMID: 18826340) Mol Ther. 2010 Apr;18(4):700-6. (PMID: 20051940) Tissue Eng Part B Rev. 2011 Aug;17(4):281-99. (PMID: 21510824) Mater Sci Eng C Mater Biol Appl. 2013 Dec 1;33(8):4849-58. (PMID: 24094196) J Virol. 1998 Nov;72(11):8463-71. (PMID: 9765382) J Orthop Res. 2007 Oct;25(10):1261-8. (PMID: 17551972) Biomaterials. 2007 Dec;28(34):5121-7. (PMID: 17698189) J Biomed Mater Res. 1999 Oct;47(1):46-53. (PMID: 10400879) Biomaterials. 2011 Apr;32(12):3330-9. (PMID: 21296411) Adv Drug Deliv Rev. 2006 Jul 7;58(4):487-99. (PMID: 16762443) Mol Ther. 2007 Jun;15(6):1114-20. (PMID: 17440444) Trends Biotechnol. 2005 Jan;23(1):42-7. (PMID: 15629857) Proc Natl Acad Sci U S A. 2008 Aug 26;105(34):12170-5. (PMID: 18719120) Soft Matter. 2012 Jan 1;8:10409-10418. (PMID: 23908674) Connect Tissue Res. 2012;53(2):95-105. (PMID: 22185608) J Biomech Eng. 1980 Feb;102(1):73-84. (PMID: 7382457) Biomaterials. 2013 Sep;34(28):6938-48. (PMID: 23777912) Mol Ther. 2006 Feb;13(2):382-90. (PMID: 16275162) Mol Ther. 2003 Apr;7(4):460-6. (PMID: 12727108) Adv Drug Deliv Rev. 2007 May 30;59(4-5):263-73. (PMID: 17507111) Tissue Eng Part A. 2010 Apr;16(4):1291-301. (PMID: 19903085) Nat Mater. 2007 Feb;6(2):162-7. (PMID: 17237789) Mol Ther. 2011 Aug;19(8):1407-15. (PMID: 21629221) Hum Gene Ther. 2001 Oct 10;12(15):1893-905. (PMID: 11589831) Biomaterials. 2009 Oct;30(29):5785-92. (PMID: 19596152) Biomaterials. 2007 Jul;28(21):3228-35. (PMID: 17448533) J Control Release. 2009 May 5;135(3):250-8. (PMID: 19331850) J Biomech Eng. 2000 Dec;122(6):570-5. (PMID: 11192376) Nat Methods. 2006 Feb;3(2):109-16. (PMID: 16432520) Tissue Eng Part A. 2013 Jun;19(11-12):1285-94. (PMID: 23289669) Lancet. 2010 Aug 7;376(9739):440-8. (PMID: 20692530) Arthritis Rheum. 2008 May;58(5):1377-88. (PMID: 18438858) Biomaterials. 2009 Aug;30(22):3790-9. (PMID: 19395019) Cell Tissue Res. 2012 Mar;347(3):613-27. (PMID: 22030892) Chem Commun (Camb). 2010 Jul 14;46(26):4743-5. (PMID: 20485844) Nucleic Acids Res. 2001 May 1;29(9):e45. (PMID: 11328886) Tissue Eng Part B Rev. 2014 Apr;20(2):106-25. (PMID: 23815376) Tissue Eng Part A. 2012 Apr;18(7-8):715-24. (PMID: 21988555) Curr Gene Ther. 2008 Dec;8(6):474-82. (PMID: 19075630) Mol Med. 2012 May 09;18:346-58. (PMID: 22160392) Biochim Biophys Acta. 1986 Sep 4;883(2):173-7. (PMID: 3091074) J Mol Med (Berl). 2013 May;91(5):625-36. (PMID: 23149825) Arch Biochem Biophys. 1961 May;93:440-7. (PMID: 13786180) Gene Ther. 2007 Jun;14(11):891-901. (PMID: 17344901) Biophys Chem. 2004 Dec 20;112(2-3):267-76. (PMID: 15572258) Mech Dev. 2000 Apr;92(2):193-205. (PMID: 10727858) Biomaterials. 2010 May;31(15):4353-9. (PMID: 20206382) J Biol Chem. 1986 May 5;261(13):5693-5. (PMID: 3754555) J Biomed Mater Res A. 2010 Jun 15;93(4):1252-9. (PMID: 19827108) Tissue Eng. 1998 Winter;4(4):415-28. (PMID: 9916173) J Cell Biol. 1997 Oct 20;139(2):541-52. (PMID: 9334355) Ann Plast Surg. 2009 May;62(5):505-12. (PMID: 19387150) J Orthop Res. 1997 May;15(3):463-7. (PMID: 9246095) Biomaterials. 2000 Dec;21(24):2575-87. (PMID: 11071607) Mol Ther. 2008 Mar;16(3):466-73. (PMID: 18180771) J Virol. 1998 Dec;72(12):9873-80. (PMID: 9811723) Eur Cell Mater. 2013 Apr 16;25:229-47. (PMID: 23588785) Nat Biotechnol. 1999 Jun;17(6):551-4. (PMID: 10385318) Biomaterials. 2012 Dec;33(35):8967-74. (PMID: 22999467) Annu Rev Biomed Eng. 2007;9:87-119. (PMID: 17425467) Mol Ther. 2005 Aug;12(2):219-28. (PMID: 16043093) Proc Inst Mech Eng H. 2007 Jul;221(5):441-50. (PMID: 17822146) Nat Methods. 2009 May;6(5):370-6. (PMID: 19404254) |
معلومات مُعتمدة: | OD008586 United States OD NIH HHS; AG15768 United States AG NIA NIH HHS; R01 AR048852 United States AR NIAMS NIH HHS; R01 AG046927 United States AG NIA NIH HHS; R01 AR048182 United States AR NIAMS NIH HHS; P01 CA47741 United States CA NCI NIH HHS; AG46927 United States AG NIA NIH HHS; P01 CA047741 United States CA NCI NIH HHS; AR48852 United States AR NIAMS NIH HHS; R03 AR061042 United States AR NIAMS NIH HHS; T32 HD040372 United States HD NICHD NIH HHS; DP2 OD008586 United States OD NIH HHS; AR50245 United States AR NIAMS NIH HHS; P01 AR050245 United States AR NIAMS NIH HHS; AR061042 United States AR NIAMS NIH HHS; R01 AG015768 United States AG NIA NIH HHS; AR48182 United States AR NIAMS NIH HHS |
فهرسة مساهمة: | Keywords: biomaterials; chondrocyte; gene therapy; genetic engineering; regenerative medicine |
المشرفين على المادة: | 0 (DNA Primers) 0 (Polyesters) 0 (Transforming Growth Factor beta3) 24980-41-4 (polycaprolactone) 25104-18-1 (Polylysine) |
تواريخ الأحداث: | Date Created: 20140220 Date Completed: 20140508 Latest Revision: 20211021 |
رمز التحديث: | 20221213 |
مُعرف محوري في PubMed: | PMC3948308 |
DOI: | 10.1073/pnas.1321744111 |
PMID: | 24550481 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1091-6490 |
---|---|
DOI: | 10.1073/pnas.1321744111 |