دورية أكاديمية
أعرض في EDS

High Rhodotorula sequences in skin transcriptome of patients with diffuse systemic sclerosis.

التفاصيل البيبلوغرافية
العنوان: High Rhodotorula sequences in skin transcriptome of patients with diffuse systemic sclerosis.
المؤلفون: Arron ST; Department of Dermatology, University of California, San Francisco, San Francisco, California, USA. Electronic address: arrons@derm.ucsf.edu., Dimon MT; Department of Dermatology, University of California, San Francisco, San Francisco, California, USA., Li Z; Department of Genetics, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA., Johnson ME; Department of Genetics, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA., Wood TA; Department of Genetics, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA., Feeney L; Department of Dermatology, University of California, San Francisco, San Francisco, California, USA., Angeles JG; Department of Dermatology, University of California, San Francisco, San Francisco, California, USA., Lafyatis R; Department of Medicine, Boston University School of Medicine, Boston, Massachusetts, USA., Whitfield ML; Department of Genetics, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA. Electronic address: michael.L.whitfield@dartmouth.edu.
المصدر: The Journal of investigative dermatology [J Invest Dermatol] 2014 Aug; Vol. 134 (8), pp. 2138-2145. Date of Electronic Publication: 2014 Mar 07.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: United States NLM ID: 0426720 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1523-1747 (Electronic) Linking ISSN: 0022202X NLM ISO Abbreviation: J Invest Dermatol Subsets: MEDLINE
أسماء مطبوعة: Publication: 2016- : New York : Elsevier
Original Publication: Baltimore, Williams & Wilkins.
مواضيع طبية MeSH: Transcriptome*, Rhodotorula/*isolation & purification , Scleroderma, Diffuse/*microbiology , Skin/*microbiology, Aged ; Female ; Humans ; Male ; Metagenomics ; Middle Aged ; RNA, Ribosomal, 28S/genetics ; Rhodotorula/genetics
مستخلص: Previous studies have suggested a role for pathogens as a trigger of systemic sclerosis (SSc), although neither a pathogen nor a mechanism of pathogenesis is known. Here we show enrichment of Rhodotorula sequences in the skin of patients with early, diffuse SSc compared with that in normal controls. RNA-seq was performed on four SSc patients and four controls, to a depth of 200 million reads per patient. Data were analyzed to quantify the nonhuman sequence reads in each sample. We found little difference between bacterial microbiome and viral read counts, but found a significant difference between the read counts for a mycobiome component, R. glutinis. Normal samples contained almost no detected R. glutinis or other Rhodotorula sequence reads (mean score 0.021 for R. glutinis, 0.024 for all Rhodotorula). In contrast, SSc samples had a mean score of 5.039 for R. glutinis (5.232 for Rhodotorula). We were able to assemble the D1-D2 hypervariable region of the 28S ribosomal RNA (rRNA) of R. glutinis from each of the SSc samples. Taken together, these results suggest that R. glutinis may be present in the skin of early SSc patients at higher levels than in normal skin, raising the possibility that it may be triggering the inflammatory response found in SSc.
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معلومات مُعتمدة: KL2 TR000143 United States TR NCATS NIH HHS; R01 AR051089 United States AR NIAMS NIH HHS; R01 AR061384 United States AR NIAMS NIH HHS; P50 AR060780 United States AR NIAMS NIH HHS; P30 AR061271 United States AR NIAMS NIH HHS
المشرفين على المادة: 0 (RNA, Ribosomal, 28S)
تواريخ الأحداث: Date Created: 20140311 Date Completed: 20140929 Latest Revision: 20211021
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC4102619
DOI: 10.1038/jid.2014.127
PMID: 24608988
قاعدة البيانات: MEDLINE
الوصف
تدمد:1523-1747
DOI:10.1038/jid.2014.127