دورية أكاديمية
أعرض في EDS

MicroRNA regulation of endothelin-1 mRNA in renal collecting duct cells.

التفاصيل البيبلوغرافية
العنوان: MicroRNA regulation of endothelin-1 mRNA in renal collecting duct cells.
المؤلفون: Jacobs ME; Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL 32610, USA., Jeffers LA; Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL 32610, USA., Welch AK; Department of Nephrology, University of Florida, Gainesville, FL 32610, USA; North Florida/South Georgia Veterans Health System, Gainesville, FL 32610, USA., Wingo CS; Department of Nephrology, University of Florida, Gainesville, FL 32610, USA; North Florida/South Georgia Veterans Health System, Gainesville, FL 32610, USA., Cain BD; Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL 32610, USA. Electronic address: bcain@ufl.edu.
المصدر: Life sciences [Life Sci] 2014 Nov 24; Vol. 118 (2), pp. 195-9. Date of Electronic Publication: 2014 Mar 13.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0375521 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0631 (Electronic) Linking ISSN: 00243205 NLM ISO Abbreviation: Life Sci Subsets: MEDLINE
أسماء مطبوعة: Publication: <2008->: Amsterdam : Elsevier
Original Publication: Oxford; Elmsford, N. Y. [etc.] Pergamon Press.
مواضيع طبية MeSH: Endothelin-1/*genetics , Kidney Tubules, Collecting/*cytology , Kidney Tubules, Collecting/*metabolism , MicroRNAs/*metabolism, 3' Untranslated Regions/genetics ; Aldosterone/pharmacology ; Animals ; Argonaute Proteins/metabolism ; Binding Sites ; Cell Line ; Endothelin-1/metabolism ; Gene Expression Regulation/drug effects ; Immunoprecipitation ; Kidney Tubules, Collecting/drug effects ; Mice ; MicroRNAs/genetics ; Oligonucleotide Array Sequence Analysis ; RNA, Messenger/metabolism ; Reverse Transcriptase Polymerase Chain Reaction
مستخلص: Aims: Recently, microRNAs (miRNAs) have been implicated in control of Edn1 mRNA in several tissues. Here we examined the role of miRNA action on Edn1 mRNA expression in renal distal collecting duct cells.
Main Methods: A microarray study was conducted to provide a comprehensive assessment of miRNAs present in a murine inner medullary collecting duct (mIMCD-3) cell line. The experiment was designed as a comparison between mIMCD-3 cells grown in the presence and absence of aldosterone. Argonaute (Ago) immunoprecipitation experiments were used to investigate binding of the RNA induced silencing complex (RISC) to Edn1 mRNA.
Key Findings: Thirty-four miRNAs were detected in very high abundance in mIMCD-3 cells, and a large number of others were present at lower levels. The microarray experiments were validated by quantitative PCR analysis of selected miRNAs. The microarray data, in combination with in silico examination of the Edn1 3' UTR provided a panel of candidate miRNAs that could act upon the Edn1 expression. Edn1 mRNA was co-immunoprecipitated with an Argonaute protein antibody, and this interaction was blocked by anti-miR-709 oligonucleotides.
Significance: These results define the miRNA landscape of the mIMCD-3 cell line. Moreover, Edn1 was shown to interact with Argonaute protein suggesting that it is a target of the RNA induced silencing complex (RISC).
(Copyright © 2014 Elsevier Inc. All rights reserved.)
References: Br J Pharmacol. 2005 Jan;144(1):115-22. (PMID: 15644875)
BMC Genomics. 2008 Dec 23;9:624. (PMID: 19102782)
Br J Pharmacol. 2013 Jan;168(2):327-34. (PMID: 22220553)
Physiol Rev. 2011 Jan;91(1):1-77. (PMID: 21248162)
Biochem J. 2005 May 1;387(Pt 3):763-72. (PMID: 15595926)
J Biol Chem. 2009 Oct 30;284(44):30087-96. (PMID: 19638349)
J Biol Chem. 2004 Mar 5;279(10):8655-67. (PMID: 14660616)
Front Physiol. 2013 Feb 19;4:22. (PMID: 23424003)
J Immunol. 2009 Oct 15;183(8):5232-43. (PMID: 19783678)
J Clin Invest. 2004 Aug;114(4):504-11. (PMID: 15314687)
Am J Physiol Renal Physiol. 2003 Oct;285(4):F664-73. (PMID: 12770840)
Am J Physiol Renal Physiol. 2013 Aug 15;305(4):F568-73. (PMID: 23698114)
FASEB J. 2011 Jan;25(1):16-28. (PMID: 20837776)
Urology. 2010 Apr;75(4):835-41. (PMID: 20035975)
ISRN Endocrinol. 2012;2012:549875. (PMID: 22900199)
FEBS Lett. 2012 Apr 5;586(7):1014-21. (PMID: 22569256)
J Am Soc Nephrol. 2010 Mar;21(3):438-47. (PMID: 20056746)
J Biol Chem. 2007 Aug 17;282(33):24262-9. (PMID: 17588946)
Nephron Exp Nephrol. 2003;95(2):e55-61. (PMID: 14610324)
Steroids. 2012 Apr;77(5):360-6. (PMID: 22209709)
Genome Biol. 2010;11(8):R90. (PMID: 20799968)
معلومات مُعتمدة: 1R01DK02680 United States DK NIDDK NIH HHS; 5T32DK076541 United States DK NIDDK NIH HHS; R01 DK082680 United States DK NIDDK NIH HHS; T32 DK007357 United States DK NIDDK NIH HHS; T32 HL083810 United States HL NHLBI NIH HHS; T32 DK076541 United States DK NIDDK NIH HHS
فهرسة مساهمة: Keywords: Endothelin-1; Kidney; miRNA
المشرفين على المادة: 0 (3' Untranslated Regions)
0 (Argonaute Proteins)
0 (Endothelin-1)
0 (MicroRNAs)
0 (RNA, Messenger)
4964P6T9RB (Aldosterone)
تواريخ الأحداث: Date Created: 20140318 Date Completed: 20150930 Latest Revision: 20211021
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC4163143
DOI: 10.1016/j.lfs.2014.03.003
PMID: 24632479
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0631
DOI:10.1016/j.lfs.2014.03.003