دورية أكاديمية
Structural modifications and tissue response after standard epi-off and iontophoretic corneal crosslinking with different irradiation procedures.
| العنوان: | Structural modifications and tissue response after standard epi-off and iontophoretic corneal crosslinking with different irradiation procedures. |
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| المؤلفون: | Mastropasqua L; Department of Medicine and Ageing Sciences, Ophthalmology Clinic, University 'G. d'Annunzio' of Chieti-Pescara, Chieti and Pescara, Italy., Lanzini M, Curcio C, Calienno R, Mastropasqua R, Colasante M, Mastropasqua A, Nubile M |
| المصدر: | Investigative ophthalmology & visual science [Invest Ophthalmol Vis Sci] 2014 Apr 17; Vol. 55 (4), pp. 2526-33. Date of Electronic Publication: 2014 Apr 17. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Association For Research In Vision And Ophthalmology (Arvo) Country of Publication: United States NLM ID: 7703701 Publication Model: Electronic Cited Medium: Internet ISSN: 1552-5783 (Electronic) Linking ISSN: 01460404 NLM ISO Abbreviation: Invest Ophthalmol Vis Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Brookline Ma : Association For Research In Vision And Ophthalmology (Arvo) Original Publication: St. Louis, Mosby. |
| مواضيع طبية MeSH: | Ultraviolet Rays*, Collagen/*metabolism , Cornea/*pathology , Cross-Linking Reagents/*pharmacology , Iontophoresis/*methods , Keratoconus/*therapy, Cadaver ; Cornea/drug effects ; Cornea/radiation effects ; Humans ; Immunohistochemistry ; Keratoconus/metabolism ; Keratoconus/pathology |
| مستخلص: | Purpose: The aim of this study is to investigate modifications in human cadaver corneas after different crosslinking procedures, including standard epi-off treatment, iontophoresis imbibition, and different exposure to ultraviolet A (UVA) sources (30 minutes at 3 mW and 9 minutes at 10 mW). Methods: A total of 12 human cadaver corneas was examined and divided as follows: 3 served as control (group 1), 3 were treated with a standard epi-off procedure (group 2), 6 underwent iontophoresis imbibition for 5 minutes, and then 3 were irradiated for 30 minutes with 3 mW UVA (group 3), and 3 for 9 minutes at 10 mW UVA (group 4). Deformation amplitude index was measured before and after the corneas underwent treatment. After treatment, corneas were prepared for hematoxylin-eosin and immunohistochemistry evaluation. The expression of TUNEL, matrix metalloproteinase-1 (MMP-1), collagen type I, and CD34 was investigate in all samples. Results: The deformation amplitude index decreased in all groups, in particular in group 4, indicating an improvement of corneal biomechanical properties. Immunohistochemical staining showed a significant stromal alteration in group 2, mild damage in group 3, and no modifications in corneal morphology in group 4. The TUNEL (P < 0.001) and MMP-1 (P = 0.002) positivity was more evident in group 4. Collagen type I positivity significantly increased in groups 3 (P = 0.002) and 4 (P = 0.002). The CD34 expression was more evident in groups 2 (P = 0.003) and 3 (P = 0.003). Conclusions: Iontophoresis imbibition followed by UVA irradiation for 9 minutes at 10 mW determined less tissue damage and better stromal remodeling. |
| فهرسة مساهمة: | Keywords: crosslinking; iontophoresis; keratoconus |
| المشرفين على المادة: | 0 (Cross-Linking Reagents) 9007-34-5 (Collagen) |
| تواريخ الأحداث: | Date Created: 20140327 Date Completed: 20140520 Latest Revision: 20140418 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1167/iovs.13-13363 |
| PMID: | 24667859 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1552-5783 |
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| DOI: | 10.1167/iovs.13-13363 |