دورية أكاديمية
Ureaplasma parvum prosthetic joint infection detected by PCR.
| العنوان: | Ureaplasma parvum prosthetic joint infection detected by PCR. |
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| المؤلفون: | Farrell JJ; University of Illinois School of Medicine, Department of Medicine, Peoria, Illinois, USA jjf@uic.edu., Larson JA; Division of Laboratory Medicine, St. Francis Medical Center, Peoria, Illinois, USA., Akeson JW; University of Illinois School of Medicine, Department of Orthopedic Surgery, Peoria, Illinois, USA., Lowery KS; Ibis Biosciences, Carlsbad, California, USA., Rounds MA; Ibis Biosciences, Carlsbad, California, USA., Sampath R; Ibis Biosciences, Carlsbad, California, USA., Bonomo RA; Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Research Service, Cleveland, Ohio, USA Case Western Reserve University, Departments of Medicine, Pharmacology and Molecular Microbiology, Cleveland, Ohio, USA., Patel R; Divisions of Clinical Microbiology and Infectious Diseases, Mayo Clinic, Rochester, Minnesota, USA. |
| المصدر: | Journal of clinical microbiology [J Clin Microbiol] 2014 Jun; Vol. 52 (6), pp. 2248-50. Date of Electronic Publication: 2014 Mar 26. |
| نوع المنشور: | Case Reports; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7505564 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-660X (Electronic) Linking ISSN: 00951137 NLM ISO Abbreviation: J Clin Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Society for Microbiology. |
| مواضيع طبية MeSH: | Polymerase Chain Reaction*, Arthritis/*diagnosis , Prosthesis-Related Infections/*diagnosis , Ureaplasma/*isolation & purification , Ureaplasma Infections/*diagnosis, Aged ; Arthritis/microbiology ; Arthritis/pathology ; Arthroplasty, Replacement, Knee/adverse effects ; Humans ; Male ; Molecular Diagnostic Techniques ; Prosthesis-Related Infections/microbiology ; Prosthesis-Related Infections/pathology ; Spectrometry, Mass, Electrospray Ionization ; Synovial Fluid/microbiology ; United States ; Ureaplasma Infections/microbiology ; Ureaplasma Infections/pathology |
| مستخلص: | We describe the first reported case of Ureaplasma parvum prosthetic joint infection (PJI) detected by PCR. Ureaplasma species do not possess a cell wall and are usually associated with colonization and infection of mucosal surfaces (not prosthetic material). U. parvum is a relatively new species name for certain serovars of Ureaplasma urealyticum, and PCR is useful for species determination. Our patient presented with late infection of his right total knee arthroplasty. Intraoperative fluid and tissue cultures and pre- and postoperative synovial fluid cultures were all negative. To discern the pathogen, we employed PCR coupled with electrospray ionization mass spectrometry (PCR/ESI-MS). Our patient's failure to respond to empirical antimicrobial treatment and our previous experience with PCR/ESI-MS in culture-negative cases of infection prompted us to use this approach over other diagnostic modalities. PCR/ESI-MS detected U. parvum in all samples. U. parvum-specific PCR testing was performed on all synovial fluid samples to confirm the U. parvum detection. (Copyright © 2014, American Society for Microbiology. All Rights Reserved.) |
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| معلومات مُعتمدة: | R01 AI100560 United States AI NIAID NIH HHS; R01AI100560 United States AI NIAID NIH HHS; R01 AI072219 United States AI NIAID NIH HHS; R01 AI063517 United States AI NIAID NIH HHS; R01AI063517 United States AI NIAID NIH HHS; R01AR056647 United States AR NIAMS NIH HHS; R01AI072219 United States AI NIAID NIH HHS; R01 AR056647 United States AR NIAMS NIH HHS |
| تواريخ الأحداث: | Date Created: 20140328 Date Completed: 20150105 Latest Revision: 20211021 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC4042745 |
| DOI: | 10.1128/JCM.00432-14 |
| PMID: | 24671783 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1098-660X |
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| DOI: | 10.1128/JCM.00432-14 |