دورية أكاديمية
Differential sRNA regulation in leaves and roots of sugarcane under water depletion.
العنوان: | Differential sRNA regulation in leaves and roots of sugarcane under water depletion. |
---|---|
المؤلفون: | Thiebaut F; Laboratório de Biologia Molecular de Plantas, Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil., Grativol C; Laboratório de Biologia Molecular de Plantas, Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil., Tanurdzic M; School of Biological Sciences, The University of Queensland, Brisbane St Lucia, Queensland, Australia., Carnavale-Bottino M; Laboratório de Biologia Molecular de Plantas, Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil., Vieira T; Laboratório de Biologia Molecular de Plantas, Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil., Motta MR; Laboratório de Biologia Molecular de Plantas, Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil., Rojas C; Universidade Federal da INTEGRAÇÃO Latino-Americana, Foz do Iguaçu, Paraná, Brazil., Vincentini R; Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Campinas, São Paulo, Brazil., Chabregas SM; Centro de Tecnologia Canavieira, Fazenda Santo Antônio - Laboratório de Biologia Molecular, Piracicaba, São Paulo, Brazil., Hemerly AS; Laboratório de Biologia Molecular de Plantas, Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil., Martienssen RA; Howard Hughes Medical Institute and Gordon and Betty Moore Foundation, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, United States of America., Ferreira PC; Laboratório de Biologia Molecular de Plantas, Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil. |
المصدر: | PloS one [PLoS One] 2014 Apr 02; Vol. 9 (4), pp. e93822. Date of Electronic Publication: 2014 Apr 02 (Print Publication: 2014). |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: San Francisco, CA : Public Library of Science |
مواضيع طبية MeSH: | Droughts*, Gene Expression Regulation, Plant/*physiology , Plant Leaves/*metabolism , Plant Roots/*metabolism , RNA, Plant/*metabolism , Saccharum/*genetics , Stress, Physiological/*physiology, RNA, Plant/genetics ; Saccharum/metabolism |
مستخلص: | Plants have developed multiple regulatory mechanisms to respond and adapt to stress. Drought stress is one of the major constraints to agricultural productivity worldwide and recent reports have highlighted the importance of plant sRNA in the response and adaptation to water availability. In order to increase our understanding of the roles of sRNA in response to water depletion, cultivars of sugarcane were submitted to treatment of ceasing drip irrigation for 24 hours. Deep sequencing analysis was carried out to identify the sRNA regulated in leaves and roots of sugarcane cultivars with different drought sensitivities. The pool of sRNA selected allowed the analysis of different sRNA classes (miRNA and siRNA). Twenty-eight and 36 families of conserved miRNA were identified in leaf and root libraries, respectively. Dynamic regulation of miRNA was observed and the expression profiles of eight miRNA were verified in leaf samples from three biological replicates by stem-loop qRT-PCR assay using the cultivars: SP90-1638--sensitive cultivar--and SP83-2847 and SP83-5073--tolerant cultivars. Altered miRNA regulation was correlated with changes in mRNA levels of specific targets. Two leaf libraries from individual sugarcane cultivars with contrasting drought-tolerance properties were also analyzed. An enrichment of 22-nt sRNA species was observed in leaf libraries. 22-nt miRNA triggered siRNA production by cleavage of their targets in response to water depletion. A number of genes of the sRNA biogenesis pathway were down-regulated in tolerant genotypes and up-regulated in sensitive in response to water depletion treatment. Our analysis contributes to increase the knowledge on the roles of sRNA in sugarcane submitted to water depletion. |
References: | Plant Cell. 2010 Jan;22(1):17-33. (PMID: 20086188) Cell. 2010 Jan 8;140(1):111-22. (PMID: 20085706) BMC Plant Biol. 2010 Nov 24;10:260. (PMID: 21092324) Plant J. 2006 Apr;46(2):243-59. (PMID: 16623887) Trends Plant Sci. 2008 Jul;13(7):350-8. (PMID: 18508405) Proc Natl Acad Sci U S A. 2005 Aug 16;102(33):11928-33. (PMID: 16081530) Plant Cell. 2010 Feb;22(2):321-34. (PMID: 20173091) Bioinformatics. 2008 Oct 1;24(19):2252-3. (PMID: 18713789) Plant Methods. 2007 Oct 12;3:12. (PMID: 17931426) Funct Integr Genomics. 2009 Nov;9(4):419-32. (PMID: 19802639) Biochim Biophys Acta. 2012 Feb;1819(2):176-85. (PMID: 21914492) Funct Integr Genomics. 2009 Aug;9(3):277-86. (PMID: 19221817) Gene. 2010 Jul 1;459(1-2):39-47. (PMID: 20350593) Plant Cell. 2002 Mar;14(3):629-39. (PMID: 11910010) EMBO J. 2007 Mar 21;26(6):1691-701. (PMID: 17332757) Nature. 2001 Jan 18;409(6818):363-6. (PMID: 11201747) Science. 2005 Feb 11;307(5711):932-5. (PMID: 15705854) BMC Plant Biol. 2008 Apr 16;8:37. (PMID: 18416839) J Exp Bot. 2011 Jul;62(11):3765-79. (PMID: 21511902) Biochim Biophys Acta. 2012 Feb;1819(2):137-48. (PMID: 21605713) PLoS Genet. 2007 Jun;3(6):e86. (PMID: 17542647) Curr Opin Plant Biol. 2009 Apr;12(2):133-9. (PMID: 19179104) Dev Biol. 2006 Jan 1;289(1):3-16. (PMID: 16325172) BMC Genomics. 2012 Jul 02;13:290. (PMID: 22747909) Ann Bot. 2004 Oct;94(4):481-95. (PMID: 15319229) Proc Natl Acad Sci U S A. 2010 Aug 24;107(34):15269-74. (PMID: 20643946) Mol Cell. 2011 May 6;42(3):356-66. (PMID: 21549312) Curr Opin Plant Biol. 2005 Feb;8(1):38-44. (PMID: 15653398) Trends Plant Sci. 2010 Jun;15(6):337-45. (PMID: 20427224) Science. 2003 Jan 31;299(5607):716-9. (PMID: 12522258) Proc Natl Acad Sci U S A. 2008 Jul 22;105(29):9970-5. (PMID: 18632569) Cell. 2007 Mar 23;128(6):1089-103. (PMID: 17346786) Plant Cell Rep. 2011 Aug;30(8):1383-91. (PMID: 21476089) Nat Genet. 2006 Jun;38 Suppl:S31-6. (PMID: 16736022) Curr Opin Plant Biol. 2011 Jun;14(3):267-74. (PMID: 21450514) Nucleic Acids Res. 2005 Nov 27;33(20):e179. (PMID: 16314309) Cell. 2006 Nov 3;127(3):565-77. (PMID: 17081978) Cell. 2005 Apr 22;121(2):207-21. (PMID: 15851028) Trends Plant Sci. 2007 Jul;12(7):301-9. (PMID: 17573231) Planta. 2009 Mar;229(4):1009-14. (PMID: 19148671) PLoS One. 2011;6(5):e19523. (PMID: 21603611) RNA. 2006 Feb;12(2):206-12. (PMID: 16428603) PLoS One. 2013;8(3):e59423. (PMID: 23544066) BMC Genomics. 2011 Jul 15;12:367. (PMID: 21762498) Curr Opin Plant Biol. 2011 Apr;14(2):148-55. (PMID: 21159545) Plant Physiol. 2009 Dec;151(4):2120-32. (PMID: 19854858) Methods Mol Biol. 2010;592:203-30. (PMID: 19802598) Proc Natl Acad Sci U S A. 2005 Mar 8;102(10):3691-6. (PMID: 15738428) J Plant Physiol. 2004 Nov;161(11):1189-202. (PMID: 15602811) J Exp Bot. 2011 Mar;62(6):1951-60. (PMID: 21193578) Nat Struct Mol Biol. 2007 Sep;14(9):869-71. (PMID: 17676062) Arabidopsis Book. 2011;9:e0146. (PMID: 22303271) PLoS Biol. 2004 May;2(5):E104. (PMID: 15024409) Trends Plant Sci. 2012 Jun;17(6):369-81. (PMID: 22445067) Cell. 2002 Aug 23;110(4):513-20. (PMID: 12202040) Biochem Biophys Res Commun. 2009 Feb 20;379(4):985-9. (PMID: 19135985) PLoS Genet. 2008 Dec;4(12):e1000314. (PMID: 19096705) Mol Cell. 2010 May 14;38(3):465-75. (PMID: 20381393) Annu Rev Plant Biol. 2009;60:485-510. (PMID: 19519217) PLoS One. 2012;7(10):e46703. (PMID: 23071617) Genome Biol. 2011 Jun 16;12(6):R53. (PMID: 21679406) Genes Dev. 2011 Dec 1;25(23):2540-53. (PMID: 22156213) Semin Cell Dev Biol. 2010 Oct;21(8):798-804. (PMID: 20359543) Nat Struct Mol Biol. 2010 Aug;17(8):997-1003. (PMID: 20562854) Curr Opin Plant Biol. 2002 Apr;5(2):122-7. (PMID: 11856607) BMC Genomics. 2010 Jun 29;11:408. (PMID: 20584339) J Biomed Biotechnol. 2009;2009:952304. (PMID: 19859540) J Exp Bot. 2010 Oct;61(15):4157-68. (PMID: 20729483) Genome Biol. 2012 Jun 15;13(6):R47. (PMID: 22704043) Cell. 2004 Jan 23;116(2):281-97. (PMID: 14744438) Curr Biol. 2004 Feb 17;14(4):346-51. (PMID: 14972688) BMC Genomics. 2010 Feb 12;11:109. (PMID: 20152027) Plant Cell Environ. 2012 Mar;35(3):502-12. (PMID: 22017483) Trends Plant Sci. 2012 Apr;17(4):196-203. (PMID: 22365280) BMC Genomics. 2008 Dec 09;9:593. (PMID: 19068109) Curr Biol. 2004 Jul 13;14(13):1214-20. (PMID: 15242620) Plant Cell. 2005 May;17(5):1376-86. (PMID: 15829603) Genomics Proteomics Bioinformatics. 2011 Dec;9(6):183-99. (PMID: 22289475) Genome Res. 2012 Jan;22(1):163-76. (PMID: 21940835) Proc Natl Acad Sci U S A. 2008 Sep 30;105(39):14958-63. (PMID: 18815367) |
معلومات مُعتمدة: | R01 GM067014 United States GM NIGMS NIH HHS |
المشرفين على المادة: | 0 (RNA, Plant) |
تواريخ الأحداث: | Date Created: 20140404 Date Completed: 20150129 Latest Revision: 20231110 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC3973653 |
DOI: | 10.1371/journal.pone.0093822 |
PMID: | 24695493 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1932-6203 |
---|---|
DOI: | 10.1371/journal.pone.0093822 |