دورية أكاديمية
Flow-through synthesis on Teflon-patterned paper to produce peptide arrays for cell-based assays.
| العنوان: | Flow-through synthesis on Teflon-patterned paper to produce peptide arrays for cell-based assays. |
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| المؤلفون: | Deiss F; Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2 (Canada)., Matochko WL, Govindasamy N, Lin EY, Derda R |
| المصدر: | Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2014 Jun 16; Vol. 53 (25), pp. 6374-7. Date of Electronic Publication: 2014 Apr 11. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 0370543 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1521-3773 (Electronic) Linking ISSN: 14337851 NLM ISO Abbreviation: Angew Chem Int Ed Engl Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2004-> : Weinheim : Wiley-VCH Original Publication: Weinheim/Bergstr. : New York, : Verlag Chemie ; Academic Press, c1962- |
| مواضيع طبية MeSH: | Paper*, Peptides/*chemical synthesis , Polytetrafluoroethylene/*chemistry , Tissue Array Analysis/*instrumentation, Peptides/chemistry |
| مستخلص: | A simple method is described for the patterned deposition of Teflon on paper to create an integrated platform for parallel organic synthesis and cell-based assays. Solvent-repelling barriers made of Teflon-impregnated paper confine organic solvents to specific zones of the patterned array and allow for 96 parallel flow-through syntheses on paper. The confinement and flow-through mixing significantly improves the peptide yield and simplifies the automation of this synthesis. The synthesis of 100 peptides ranging from 7 to 14 amino acids in length gave over 60% purity for the majority of the peptides (>95% yield per coupling/deprotection cycle). The resulting peptide arrays were used in cell-based screening to identify 14 potent bioactive peptides that support the adhesion or proliferation of breast cancer cells in a 3D environment. In the future, this technology could be used for the screening of more complex phenotypic responses, such as cell migration or differentiation. (© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.) |
| فهرسة مساهمة: | Keywords: cell adhesion; high-throughput screening; peptide arrays; peptides; solid-phase synthesis |
| المشرفين على المادة: | 0 (Peptides) 9002-84-0 (Polytetrafluoroethylene) |
| تواريخ الأحداث: | Date Created: 20140415 Date Completed: 20150914 Latest Revision: 20140613 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1002/anie.201402037 |
| PMID: | 24729420 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1521-3773 |
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| DOI: | 10.1002/anie.201402037 |