دورية أكاديمية
أعرض في EDS

Polymorphisms and ambiguous sites present in DNA sequences of Leishmania clones: looking closer.

التفاصيل البيبلوغرافية
العنوان: Polymorphisms and ambiguous sites present in DNA sequences of Leishmania clones: looking closer.
المؤلفون: Boité MC; Laboratório de Pesquisa em Leishmaniose, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Brazil. Electronic address: boitemc@ioc.fiocruz.br., de Oliveira TS; Laboratório de Pesquisa em Leishmaniose, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Brazil., Ferreira GE; Laboratório de Pesquisa em Leishmaniose, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Brazil., Trannin M; Laboratório de Pesquisa em Leishmaniose, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Brazil., dos Santos BN; Laboratório de Pesquisa em Leishmaniose, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Brazil., Porrozzi R; Laboratório de Pesquisa em Leishmaniose, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Brazil., Cupolillo E; Laboratório de Pesquisa em Leishmaniose, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Brazil.
المصدر: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases [Infect Genet Evol] 2014 Jul; Vol. 25, pp. 110-6. Date of Electronic Publication: 2014 Apr 21.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 101084138 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1567-7257 (Electronic) Linking ISSN: 15671348 NLM ISO Abbreviation: Infect Genet Evol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam ; New York : Elsevier Science, c2001-
مواضيع طبية MeSH: Genetic Variation*, DNA, Protozoan/*genetics , Leishmania/*genetics, Evolution, Molecular ; Leishmania/classification ; Multilocus Sequence Typing ; Phylogeny ; Polymorphism, Genetic ; Protozoan Proteins/genetics ; Sequence Analysis, DNA
مستخلص: In genetic studies of Leishmania parasites, co-dominant markers are chosen for their ability to detect heterozygous polymorphisms, to infer the occurrence of inbreeding and to resolve genetic variability. The majority of DNA sequence based reports perform conventional dye terminator cycle sequencing where perfectly ambiguous sites or double peaks in the chromatogram are interpreted as heterozygous strains. However, molecular peculiarities of the parasite such as aneuploidy, mixed populations and homologous recombination advise that data from regular DNA sequence analysis should be carefully evaluated. We report here a closer look at ambiguous sites observed in 6pgd DNA sequences obtained for a multilocus sequence analysis project on Leishmania (Viannia) strains. After comparing 286 DNA sequences from biological and molecular clones of six L. (Viannia) strains we could distinguish events that contribute to genetic variation in Leishmania (recombination, mutation, chromosomal mosaics). Also, the results suggest how diversity might not be completely revealed through regular DNA sequence analysis and demonstrate the importance for molecular epidemiology research to be aware of such possibilities while choosing samples for studies.
(Copyright © 2014 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Aneuploidy; Heterozygosis; Leishmania; Polyclonal
المشرفين على المادة: 0 (DNA, Protozoan)
0 (Protozoan Proteins)
تواريخ الأحداث: Date Created: 20140429 Date Completed: 20150112 Latest Revision: 20140602
رمز التحديث: 20231215
DOI: 10.1016/j.meegid.2014.04.011
PMID: 24768683
قاعدة البيانات: MEDLINE
الوصف
تدمد:1567-7257
DOI:10.1016/j.meegid.2014.04.011