دورية أكاديمية
أعرض في EDS

Glutamine synthetase 2 is not essential for biosynthesis of compatible solutes in Halobacillus halophilus.

التفاصيل البيبلوغرافية
العنوان: Glutamine synthetase 2 is not essential for biosynthesis of compatible solutes in Halobacillus halophilus.
المؤلفون: Shiyan A; Molecular Microbiology and Bioenergetics, Institute of Molecular Biosciences, Johann Wolfgang Goethe-University of Frankfurt am Main Frankfurt am Main, Germany., Thompson M; Molecular Microbiology and Bioenergetics, Institute of Molecular Biosciences, Johann Wolfgang Goethe-University of Frankfurt am Main Frankfurt am Main, Germany., Köcher S; Molecular Microbiology and Bioenergetics, Institute of Molecular Biosciences, Johann Wolfgang Goethe-University of Frankfurt am Main Frankfurt am Main, Germany., Tausendschön M; Molecular Microbiology and Bioenergetics, Institute of Molecular Biosciences, Johann Wolfgang Goethe-University of Frankfurt am Main Frankfurt am Main, Germany., Santos H; Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa Oeiras, Portugal., Hänelt I; Molecular Microbiology and Bioenergetics, Institute of Molecular Biosciences, Johann Wolfgang Goethe-University of Frankfurt am Main Frankfurt am Main, Germany., Müller V; Molecular Microbiology and Bioenergetics, Institute of Molecular Biosciences, Johann Wolfgang Goethe-University of Frankfurt am Main Frankfurt am Main, Germany.
المصدر: Frontiers in microbiology [Front Microbiol] 2014 Apr 14; Vol. 5, pp. 168. Date of Electronic Publication: 2014 Apr 14 (Print Publication: 2014).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101548977 Publication Model: eCollection Cited Medium: Print ISSN: 1664-302X (Print) Linking ISSN: 1664302X NLM ISO Abbreviation: Front Microbiol Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Research Foundation
مستخلص: Halobacillus halophilus, a moderately halophilic bacterium isolated from salt marshes, produces various compatible solutes to cope with osmotic stress. Glutamate and glutamine are dominant compatible solutes at mild salinities. Glutamine synthetase activity in cell suspensions of Halobacillus halophilus wild type was shown to be salt dependent and chloride modulated. A possible candidate to catalyze glutamine synthesis is glutamine synthetase A2, whose transcription is stimulated by chloride. To address the role of GlnA2 in the biosynthesis of the osmolytes glutamate and glutamine, a deletion mutant (ΔglnA2) was generated and characterized in detail. We compared the pool of compatible solutes and performed transcriptional analyses of the principal genes controlling the solute production in the wild type strain and the deletion mutant. These measurements did not confirm the hypothesized role of GlnA2 in the osmolyte production. Most likely the presence of another, yet to be identified enzyme has the main contribution in the measured activity in crude extracts and probably determines the total chloride-modulated profile. The role of GlnA2 remains to be elucidated.
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فهرسة مساهمة: Keywords: Halobacillus halophilus; compatible solutes; glutamine synthetase; halophile; osmoregulation
تواريخ الأحداث: Date Created: 20140501 Date Completed: 20140430 Latest Revision: 20211021
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC3995056
DOI: 10.3389/fmicb.2014.00168
PMID: 24782854
قاعدة البيانات: MEDLINE
الوصف
تدمد:1664-302X
DOI:10.3389/fmicb.2014.00168