دورية أكاديمية
Evaluation of three herbicide resistance genes for use in genetic transformations and for potential crop protection in algae production.
| العنوان: | Evaluation of three herbicide resistance genes for use in genetic transformations and for potential crop protection in algae production. |
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| المؤلفون: | Brueggeman AJ, Kuehler D, Weeks DP |
| المصدر: | Plant biotechnology journal [Plant Biotechnol J] 2014 Sep; Vol. 12 (7), pp. 894-902. Date of Electronic Publication: 2014 May 05. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley on behalf of the Society for Experimental Biology, Association of Applied Biologists Country of Publication: England NLM ID: 101201889 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1467-7652 (Electronic) Linking ISSN: 14677644 NLM ISO Abbreviation: Plant Biotechnol J Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2014- : Oxford Wiley on behalf of the Society for Experimental Biology, Association of Applied Biologists Original Publication: [Oxford] : Blackwell Pub., c2003- |
| مواضيع طبية MeSH: | Acetyltransferases/*genetics , Chlamydomonas reinhardtii/*genetics , Herbicide Resistance/*genetics , Herbicides/*pharmacology , Oxidoreductases/*genetics , Plant Proteins/*genetics , Protoporphyrinogen Oxidase/*genetics, Amino Acid Sequence ; Chlamydomonas reinhardtii/drug effects ; Crops, Agricultural/drug effects ; Crops, Agricultural/genetics ; Glycine/analogs & derivatives ; Glycine/pharmacology ; Halogenated Diphenyl Ethers/pharmacology ; Molecular Sequence Data ; Plants, Genetically Modified/drug effects ; Pyridazines/pharmacology ; Sequence Alignment ; Transformation, Genetic ; Glyphosate |
| مستخلص: | Genes conferring resistance to the herbicides glyphosate, oxyfluorfen and norflurazon were developed and tested for use as dominant selectable markers in genetic transformation of Chlamydomonas reinhardtii and as potential tools for the protection of commercial-scale algal production facilities against contamination by organisms sensitive to these broad-spectrum herbicides. A synthetic glyphosate acetyltransferase (GAT) gene, when fitted with a strong Chlamydomonas promoter, conferred a 2.7×-fold increase in tolerance to the EPSPS inhibitor, glyphosate, in transgenic cells compared with progenitor WT cells. A mutant Chlamydomonas protoporphyrinogen oxidase (protox, PPO) gene previously shown to produce an enzyme insensitive to PPO-inhibiting herbicides, when genetically engineered, generated transgenic cells able to tolerate up to 136× higher levels of the PPO inhibitor, oxyfluorfen, than nontransformed cells. Genetic modification of the Chlamydomonas phytoene desaturase (PDS) gene-based gene sequences found in various norflurazon-resistant organisms allowed production of transgenic cells tolerant to 40× higher levels of norflurazon than nontransgenic cells. The high efficiency of all three herbicide resistance genes in producing transgenic cells demonstrated their suitability as dominant selectable markers for genetic transformation of Chlamydomonas and, potentially, other eukaryotic algae. However, the requirement for high concentrations of glyphosate and its associated negative effects on cell growth rates preclude its consideration for use in large-scale production facilities. In contrast, only low doses of norflurazon and oxyfluorfen (~1.5 μm and ~0.1 μm, respectively) are required for inhibition of cell growth, suggesting that these two herbicides may prove effective in large-scale algal production facilities in suppressing growth of organisms sensitive to these herbicides. (© 2014 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.) |
| التعليقات: | Erratum in: Plant Biotechnol J. 2015 May;13(4):e1. Bruggeman, Andrew J [corrected to Brueggeman, Andrew J]. (PMID: 27653025) |
| فهرسة مساهمة: | Keywords: Algae; Chlamydomonas reinhardtii; glyphosate; herbicide resistance; norflurazon; oxyfluorfen |
| المشرفين على المادة: | 0 (Halogenated Diphenyl Ethers) 0 (Herbicides) 0 (Plant Proteins) 0 (Pyridazines) 46GY4Y6567 (oxyfluorofen) EC 1.- (Oxidoreductases) EC 1.14.99.- (phytoene dehydrogenase) EC 1.3.3.4 (Protoporphyrinogen Oxidase) EC 2.3.1.- (Acetyltransferases) EC 2.3.1.- (glyphosate N-acetyltransferase) KES1HB07E4 (norflurazone) TE7660XO1C (Glycine) |
| تواريخ الأحداث: | Date Created: 20140507 Date Completed: 20150423 Latest Revision: 20231213 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1111/pbi.12192 |
| PMID: | 24796724 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1467-7652 |
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| DOI: | 10.1111/pbi.12192 |