دورية أكاديمية
أعرض في EDS

Immunophenotyping of normal individuals classified on the basis of human dosha prakriti.

التفاصيل البيبلوغرافية
العنوان: Immunophenotyping of normal individuals classified on the basis of human dosha prakriti.
المؤلفون: Rotti H; Division of Biotechnology, School of Life Sciences, Manipal University, Manipal, Karnataka, India., Guruprasad KP; Division of Biotechnology, School of Life Sciences, Manipal University, Manipal, Karnataka, India., Nayak J; Department of Shalyatantra/ Roganidana, Shri Dharmasthala Manjunatheshwara College of Ayurveda, Udupi, Karnataka, India., Kabekkodu SP; Division of Biotechnology, School of Life Sciences, Manipal University, Manipal, Karnataka, India., Kukreja H; Division of Biotechnology, School of Life Sciences, Manipal University, Manipal, Karnataka, India., Mallya S; Division of Biotechnology, School of Life Sciences, Manipal University, Manipal, Karnataka, India., Nayak J; Division of Biotechnology, School of Life Sciences, Manipal University, Manipal, Karnataka, India., Bhradwaj RC; Centre for Clinical Research, Institute of Ayurveda and Integrative Medicine, Bangalore, Karnataka, India., Gangadharan GG; Centre for Clinical Research, Institute of Ayurveda and Integrative Medicine, Bangalore, Karnataka, India., Prasanna BV; Division of Biotechnology, School of Life Sciences, Manipal University, Manipal, Karnataka, India., Raval R; Division of Biotechnology, School of Life Sciences, Manipal University, Manipal, Karnataka, India., Kamath A; Department of Community Medicine, Kasturba Medical College, Manipal University, Manipal, Karnataka, India., Gopinath PM; Division of Biotechnology, School of Life Sciences, Manipal University, Manipal, Karnataka, India., Kondaiah P; Department of Molecular Reproduction Development and Genetics, Indian Institute of Science, Bangalore, Karnataka, India., Satyamoorthy K; Division of Biotechnology, School of Life Sciences, Manipal University, Manipal, Karnataka, India.
المصدر: Journal of Ayurveda and integrative medicine [J Ayurveda Integr Med] 2014 Jan; Vol. 5 (1), pp. 43-9.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: United States NLM ID: 101551404 Publication Model: Print Cited Medium: Print ISSN: 0975-9476 (Print) Linking ISSN: 09759476 NLM ISO Abbreviation: J Ayurveda Integr Med Subsets: PubMed not MEDLINE
أسماء مطبوعة: Publication: 2016- : New York : Elsevier
Original Publication: Mumbai : Medknow Publications
مستخلص: Background: Human variations related to immune response and disease susceptibility is well-documented in Ayurveda. Prakriti (body constitution) is the basic constitution of an individual established at the time of birth and distinguishes variations, into three broad phenotype categories such as vata, pitta and kapha. Variation in immune response is often attributed to and measured from the difference in cluster differentiation (CD) markers expressed in lymphocytes. Currently, there are no reports available on the expression of CD markers related to prakriti.
Objective: This is a pilot study performed to evaluate a panel of lymphocyte subset CD markers in dominant prakriti individuals.
Materials and Methods: Immunophenotyping was carried out using whole blood from a total of healthy 222 subjects, who are grouped into kapha (n = 95), pitta (n = 57) and vata (n = 70) prakritis. CD markers such as CD3, CD4, CD8, CD14, CD25, CD56, CD69, CD71 and HLA-DR were analyzed using flow cytometry method. Differences between groups were analyzed using one-way ANOVA or Kruskal-Wallis analysis of variance (ANOVA) and multiple comparisons between groups were performed by Bonferroni or Mann-Whitney U test with corrections for type I error respectively. Significance was evaluated by ANOVA and Pearson's correlation.
Results: We observed a significant difference (P < 0.05) in the expression of CD markers such as CD14 (monocytes), CD25 (activated B cells) and CD56 (Natural killer cells) between different prakriti groups. CD25 and CD56 expression was significantly higher in kapha prakriti samples than other prakriti groups. Similarly, slightly higher levels of CD14 were observed in pitta prakriti samples.
Conclusion: Significant difference in the expression of CD14, CD25 and CD56 markers between three different prakriti is demonstrated. The increased level of CD25 and CD56 in kapha prakriti may indicate ability to elicit better immune response, which is in conformity with textual references in Ayurveda.
References: Int Immunopharmacol. 2004 Jun;4(6):841-9. (PMID: 15135324)
Clin Exp Immunol. 2011 Jan;163(1):33-49. (PMID: 20939860)
J Altern Complement Med. 2005 Apr;11(2):221-5. (PMID: 15865485)
Drug Discov Today. 2009 Aug;14(15-16):804-11. (PMID: 19477288)
J Transl Med. 2008 Sep 09;6:48. (PMID: 18782426)
J Altern Complement Med. 2005 Jun;11(3):385-90. (PMID: 15992219)
J Nutr. 2007 Mar;137(3 Suppl 2):773S-80S. (PMID: 17311974)
J Altern Complement Med. 2005 Apr;11(2):349-53. (PMID: 15865503)
Proc Natl Acad Sci U S A. 1993 Mar 15;90(6):2380-4. (PMID: 7681594)
Ayu. 2010 Apr;31(2):197-204. (PMID: 22131710)
Am J Pathol. 2009 Apr;174(4):1160-71. (PMID: 19246644)
Immunology. 2006 Apr;117(4):548-57. (PMID: 16556269)
Transl Res. 2009 Dec;154(6):277-87. (PMID: 19931193)
Indian J Med Sci. 2008 Oct;62(10):397-406. (PMID: 19008613)
Pediatr Res. 2000 Jul;48(1):6-11. (PMID: 10879793)
J Altern Complement Med. 2008 Jun;14(5):571-6. (PMID: 18564959)
AIDS Res Ther. 2011 Oct 03;8:35. (PMID: 21967708)
J Altern Complement Med. 2001 Oct;7(5):567-74. (PMID: 11719949)
Eur J Immunol. 2002 Mar;32(3):761-72. (PMID: 11870620)
Lancet. 2001 Jun 2;357(9270):1777-89. (PMID: 11403834)
Indian J Hum Genet. 2011 May;17 Suppl 1:S1-3. (PMID: 21747581)
Br J Nutr. 2002 Nov;88 Suppl 2:S165-77. (PMID: 12495459)
Evid Based Complement Alternat Med. 2005 Dec;2(4):465-73. (PMID: 16322803)
Ayu. 2011 Apr;32(2):141-6. (PMID: 22408293)
Evid Based Complement Alternat Med. 2011;2011:249528. (PMID: 20015960)
J Ayurveda Integr Med. 2010 Jan;1(1):26-32. (PMID: 21829298)
Mol Immunol. 2005 Feb;42(4):501-10. (PMID: 15607806)
فهرسة مساهمة: Keywords: Cluster differentiation 14; cluster differentiation 25; cluster differentiation 56; immunophenotyping; prakriti
تواريخ الأحداث: Date Created: 20140510 Date Completed: 20140509 Latest Revision: 20211021
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC4012362
DOI: 10.4103/0975-9476.128857
PMID: 24812475
قاعدة البيانات: MEDLINE
الوصف
تدمد:0975-9476
DOI:10.4103/0975-9476.128857