دورية أكاديمية
Protease and phospholipase activities of Candida spp. isolated from cutaneous candidiasis.
| العنوان: | Protease and phospholipase activities of Candida spp. isolated from cutaneous candidiasis. |
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| المؤلفون: | Ramos Lde S; Instituto Biomédico, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil; Instituto de Microbiologia Paulo de Góes and Programa de Pós-Graduação em Bioquímica - Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Barbedo LS; Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil., Braga-Silva LA; Instituto de Microbiologia Paulo de Góes and Programa de Pós-Graduação em Bioquímica - Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., dos Santos AL; Instituto de Microbiologia Paulo de Góes and Programa de Pós-Graduação em Bioquímica - Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Pinto MR; Instituto Biomédico, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil., Sgarbi DB; Instituto Biomédico, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil. Electronic address: dbridon@id.uff.br. |
| المصدر: | Revista iberoamericana de micologia [Rev Iberoam Micol] 2015 Apr-Jun; Vol. 32 (2), pp. 122-5. Date of Electronic Publication: 2014 May 20. |
| نوع المنشور: | Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier España Country of Publication: Spain NLM ID: 9425531 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2173-9188 (Electronic) Linking ISSN: 11301406 NLM ISO Abbreviation: Rev Iberoam Micol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2009-> : Madrid : Elsevier España Original Publication: Barcelona : La Asociación, [1990- |
| مواضيع طبية MeSH: | Candida/*enzymology , Candidiasis, Cutaneous/*microbiology , Fungal Proteins/*analysis , Peptide Hydrolases/*analysis , Phospholipases/*analysis, Brazil ; Candida/classification ; Candida/isolation & purification ; Candida/pathogenicity ; Humans ; Mycological Typing Techniques ; Species Specificity ; Virulence |
| مستخلص: | Background: Cases of superficial and invasive mycoses caused by emerging species of Candida have been increasingly reported over the last thirty years. The production of hydrolytic enzymes plays a central role in the fungal infective process. In Candida infections the secretion of both proteases and phospholipases are well-known virulence attributes. Aims: To determine the protease and phospholipase production from 58 human clinical isolates of Candida obtained from individuals with cutaneous candidiasis seen in the Human and Veterinary Diagnostic Mycology Sector from Universidade Federal Fluminense (UFF), Brazil, from November 2008 to August 2009. Methods: Fungal identification was performed using biochemical tests. Proteolytic activity was detected on agar plates containing bovine serum albumin, and phospholipase production was determined on egg-yolk plates. Results: The Candida species isolated were Candida parapsilosis (27.59%), Candida famata (18.96%), Candida albicans (15.52%), Candida haemulonii (12.06%), Candida ciferri (8.62%), Candida guilliermondii (6.90%), Candida tropicalis (5.17%) and Candida lipolytica (5.17%). All isolates of C. albicans produced both protease and phospholipase. As regards the isolates of non-C. albicans Candida species, 53.06% and 4.08% were able to produce protease and phospholipase, respectively. For example, the majority of isolates of C. parapsilosis (15/16) produced protease, while 40% of C. ciferri isolates (2/5) were phospholipase producers. This study shows, for the first time, that C. ciferri and C. haemulonii strains were able to produce protease. Conclusions: Collectively, our results showed that different species of Candida isolated from cutaneous lesions were able to produce proteases and/or phospholipases, which are multifunctional molecules directly involved in the infectious process of these fungi. (Copyright © 2013 Revista Iberoamericana de Micología. Published by Elsevier Espana. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Aislamientos clínicos; Candida spp.; Candidiasis cutánea; Clinical isolates; Cutaneous candidiasis; Factores de virulencia; Fosfolipasa; Phospholipase; Proteasa; Protease; Virulence factors |
| المشرفين على المادة: | 0 (Fungal Proteins) EC 3.1.- (Phospholipases) EC 3.4.- (Peptide Hydrolases) |
| تواريخ الأحداث: | Date Created: 20140524 Date Completed: 20160204 Latest Revision: 20150505 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.riam.2014.01.003 |
| PMID: | 24853474 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2173-9188 |
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| DOI: | 10.1016/j.riam.2014.01.003 |