دورية أكاديمية
أعرض في EDS

Early exposure of murine embryonic stem cells to hematopoietic cytokines differentially directs definitive erythropoiesis and cardiomyogenesis in alginate hydrogel three-dimensional cultures.

التفاصيل البيبلوغرافية
العنوان: Early exposure of murine embryonic stem cells to hematopoietic cytokines differentially directs definitive erythropoiesis and cardiomyogenesis in alginate hydrogel three-dimensional cultures.
المؤلفون: Fauzi I; 1 Biological Systems Engineering Laboratory , Department of Chemical Engineering and Chemical Technology, Imperial College London, London, United Kingdom ., Panoskaltsis N, Mantalaris A
المصدر: Stem cells and development [Stem Cells Dev] 2014 Nov 15; Vol. 23 (22), pp. 2720-9. Date of Electronic Publication: 2014 Aug 05.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 101197107 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1557-8534 (Electronic) Linking ISSN: 15473287 NLM ISO Abbreviation: Stem Cells Dev Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Larchmont, NY : Mary Ann Liebert, Inc., c2004-
مواضيع طبية MeSH: Alginates/*metabolism , Cell Differentiation/*physiology , Cytokines/*metabolism , Embryonic Stem Cells/*physiology , Erythropoiesis/*physiology , Hematopoietic Stem Cells/*metabolism , Muscle Development/*physiology, Animals ; Cell Culture Techniques/methods ; Cell Line ; Cell Line, Tumor ; Culture Media, Conditioned/metabolism ; Embryonic Stem Cells/metabolism ; Erythroid-Specific DNA-Binding Factors/metabolism ; Glucuronic Acid/metabolism ; Hematopoietic Stem Cells/physiology ; Hep G2 Cells ; Hexuronic Acids/metabolism ; Humans ; Hydrogel, Polyethylene Glycol Dimethacrylate/metabolism ; Interleukin-3/metabolism ; Kruppel-Like Transcription Factors/metabolism ; Mice ; Myocytes, Cardiac/metabolism ; Myocytes, Cardiac/physiology ; Stem Cell Factor/metabolism ; beta-Globins/metabolism ; zeta-Globins/metabolism
مستخلص: HepG2-conditioned medium (CM) facilitates early differentiation of murine embryonic stem cells (mESCs) into hematopoietic cells in two-dimensional cultures through formation of embryoid-like colonies (ELCs), bypassing embryoid body (EB) formation. We now demonstrate that three-dimensional (3D) cultures of alginate-encapsulated mESCs cultured in a rotating wall vessel bioreactor can be differentially driven toward definitive erythropoiesis and cardiomyogenesis in the absence of ELC formation. Three groups were evaluated: mESCs in maintenance medium with leukemia inhibitory factor (LIF, control) and mESCs cultured with HepG2 CM (CM1 and CM2). Control and CM1 groups were cultivated for 8 days in early differentiation medium with murine stem cell factor (mSCF) followed by 10 days in hematopoietic differentiation medium (HDM) containing human erythropoietin, m-interleukin (mIL)-3, and mSCF. CM2 cells were cultured for 18 days in HDM, bypassing early differentiation. In CM1, a fivefold expansion of hematopoietic colonies was observed at day 14, with enhancement of erythroid progenitors, hematopoietic genes (Gata-2 and SCL), erythroid genes (EKLF and β-major globin), and proteins (Gata-1 and β-globin), although ζ-globin was not expressed. In contrast, CM2 primarily produced beating colonies in standard hematopoietic colony assay and expressed early cardiomyogenic markers, anti-sarcomeric α-actinin and Gata-4. In conclusion, a scalable, automatable, integrated, 3D bioprocess for the differentiation of mESC toward definitive erythroblasts has been established. Interestingly, cardiomyogenesis was also directed in a specific protocol with HepG2 CM and hematopoietic cytokines making this platform a useful tool for the study of erythroid and cardiomyogenic development.
References: Cell Death Differ. 2004 Jul;11 Suppl 1:S37-44. (PMID: 15243580)
PLoS One. 2008 Feb 06;3(2):e1544. (PMID: 18253492)
Cell. 2012 Aug 3;150(3):590-605. (PMID: 22863011)
Biotechnol Bioeng. 2005 May 20;90(4):452-61. (PMID: 15778986)
Blood. 1981 Dec;58(6):1236-9. (PMID: 6946849)
Tissue Eng. 2007 Dec;13(12):2957-70. (PMID: 17988191)
Stem Cells. 2005 Oct;23(9):1333-42. (PMID: 16081660)
Blood. 2008 Dec 1;112(12):4475-84. (PMID: 18713948)
Blood. 1988 Sep;72(3):944-51. (PMID: 3262001)
Development. 1999 Feb;126(3):535-46. (PMID: 9876182)
Biomaterials. 2009 Feb;30(4):499-507. (PMID: 18977027)
Cell Stem Cell. 2008 Mar 6;2(3):252-63. (PMID: 18371450)
J Biochem. 2012 Dec;152(6):509-19. (PMID: 22923740)
Blood. 1995 Jul 1;86(1):156-63. (PMID: 7795222)
J Assist Reprod Genet. 1995 Feb;12(2):97-105. (PMID: 7670281)
Development. 1999 Aug;126(16):3597-605. (PMID: 10409505)
Exp Cell Res. 2008 Jun 10;314(10):2090-9. (PMID: 18452915)
Development. 2003 Sep;130(17):4217-27. (PMID: 12874139)
Mol Cell Biol. 1993 Jan;13(1):473-86. (PMID: 8417345)
Biol Reprod. 2005 Dec;73(6):1147-56. (PMID: 16079311)
Biotechnol Bioeng. 1999 Apr 20;63(2):197-205. (PMID: 10099596)
Development. 1993 Jun;118(2):489-98. (PMID: 8223275)
Stem Cells Dev. 2012 Nov 20;21(17):3152-61. (PMID: 22587789)
J Biosci Bioeng. 2005 Jul;100(1):28-35. (PMID: 16233847)
Dev Cell. 2006 Nov;11(5):723-32. (PMID: 17084363)
Endocrinology. 2010 Feb;151(2):482-91. (PMID: 20008042)
Br J Haematol. 2000 Jun;109(4):785-7. (PMID: 10929030)
Br J Haematol. 1998 Nov;103(2):317-25. (PMID: 9827900)
Blood. 1997 Aug 15;90(4):1345-64. (PMID: 9269751)
Blood. 1995 Jul 15;86(2):572-80. (PMID: 7541668)
Proc Natl Acad Sci U S A. 2005 Sep 13;102(37):13170-5. (PMID: 16141334)
J Biosci Bioeng. 2011 Jan;111(1):92-7. (PMID: 20869914)
J Biomed Mater Res A. 2003 Nov 1;67(2):376-88. (PMID: 14566778)
Development. 1998 May;125(9):1747-57. (PMID: 9521912)
Development. 1999 Nov;126(22):5073-84. (PMID: 10529424)
J Reprod Fertil. 1993 Sep;99(1):87-95. (PMID: 8283458)
Circulation. 2001 Jan 30;103(4):549-54. (PMID: 11157721)
Tissue Eng Part A. 2010 Nov;16(11):3285-98. (PMID: 20528675)
Development. 2000 Jun;127(11):2447-59. (PMID: 10804185)
Exp Physiol. 1999 Sep;84(5):917-27. (PMID: 10502659)
Stem Cells Dev. 2008 Oct;17(5):963-70. (PMID: 18564030)
Curr Opin Hematol. 2009 Jul;16(4):259-68. (PMID: 19444099)
Development. 1998 Feb;125(4):725-32. (PMID: 9435292)
Curr Opin Cell Biol. 1995 Dec;7(6):862-9. (PMID: 8608017)
Nature. 2009 Feb 12;457(7231):892-5. (PMID: 19182774)
Nature. 2004 Dec 2;432(7017):625-30. (PMID: 15577911)
Ann N Y Acad Sci. 2001 Nov;944:135-43. (PMID: 11797664)
Proc Natl Acad Sci U S A. 2008 Sep 2;105(35):13087-92. (PMID: 18755895)
PLoS One. 2008 Feb 13;3(2):e1565. (PMID: 18270562)
Nat Methods. 2007 Jun;4(6):501-9. (PMID: 17486087)
Tissue Eng. 2006 Jun;12(6):1381-92. (PMID: 16846337)
Blood. 2011 Aug 25;118(8):2296-304. (PMID: 21527522)
Stem Cells. 2004;22(3):275-82. (PMID: 15153605)
Biotechnol Bioeng. 2004 Jun 5;86(5):493-502. (PMID: 15129432)
Nat Methods. 2012 Jul;9(7):671-5. (PMID: 22930834)
Stem Cells Dev. 2009 Jan-Feb;18(1):77-91. (PMID: 18393639)
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4334-8. (PMID: 6945587)
Circ Res. 2003 Feb 7;92(2):133-5. (PMID: 12574139)
Stem Cells. 2010 Jul;28(7):1132-42. (PMID: 20506541)
J R Soc Interface. 2009 Mar 6;6(32):209-32. (PMID: 19033137)
Blood. 2007 Apr 1;109(7):2679-87. (PMID: 17148580)
Bioprocess Biosyst Eng. 2005 Oct;27(6):381-387. (PMID: 15928928)
Nature. 2009 Feb 12;457(7231):896-900. (PMID: 19212410)
المشرفين على المادة: 0 (Alginates)
0 (Culture Media, Conditioned)
0 (Cytokines)
0 (Erythroid-Specific DNA-Binding Factors)
0 (Hexuronic Acids)
0 (Interleukin-3)
0 (Kruppel-Like Transcription Factors)
0 (Stem Cell Factor)
0 (beta-Globins)
0 (erythroid Kruppel-like factor)
0 (zeta-Globins)
25852-47-5 (Hydrogel, Polyethylene Glycol Dimethacrylate)
8A5D83Q4RW (Glucuronic Acid)
تواريخ الأحداث: Date Created: 20140614 Date Completed: 20150708 Latest Revision: 20211021
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC4216522
DOI: 10.1089/scd.2014.0105
PMID: 24926614
قاعدة البيانات: MEDLINE
الوصف
تدمد:1557-8534
DOI:10.1089/scd.2014.0105