دورية أكاديمية
أعرض في EDS

MicroRNAs are required for the feature maintenance and differentiation of brown adipocytes.

التفاصيل البيبلوغرافية
العنوان: MicroRNAs are required for the feature maintenance and differentiation of brown adipocytes.
المؤلفون: Kim HJ; Cardiovascular and Metabolic Disorders, Duke-National University of Singapore Graduate Medical School, Singapore., Cho H; Whitehead Institute for Biomedical Research, Cambridge, MA Department of Biology, Massachusetts Institute of Technology, Cambridge, MA., Alexander R; Whitehead Institute for Biomedical Research, Cambridge, MA Department of Biology, Massachusetts Institute of Technology, Cambridge, MA., Patterson HC; Whitehead Institute for Biomedical Research, Cambridge, MA Department of Pathology, Brigham and Women's Hospital, Boston, MA., Gu M; Cardiovascular and Metabolic Disorders, Duke-National University of Singapore Graduate Medical School, Singapore., Lo KA; Institute of Molecular and Cell Biology, Singapore., Xu D; Cardiovascular and Metabolic Disorders, Duke-National University of Singapore Graduate Medical School, Singapore., Goh VJ; Cardiovascular and Metabolic Disorders, Duke-National University of Singapore Graduate Medical School, Singapore., Nguyen LN; Cardiovascular and Metabolic Disorders, Duke-National University of Singapore Graduate Medical School, Singapore., Chai X; Cardiovascular and Metabolic Disorders, Duke-National University of Singapore Graduate Medical School, Singapore., Huang CX; Whitehead Institute for Biomedical Research, Cambridge, MA Department of Biology, Massachusetts Institute of Technology, Cambridge, MA., Kovalik JP; Cardiovascular and Metabolic Disorders, Duke-National University of Singapore Graduate Medical School, Singapore., Ghosh S; Cardiovascular and Metabolic Disorders, Duke-National University of Singapore Graduate Medical School, Singapore., Trajkovski M; University of Geneva, Medical Faculty, Department of Cell Physiology and Metabolism, Centre Médical Universitaire (CMU), Geneva, Switzerland., Silver DL; Cardiovascular and Metabolic Disorders, Duke-National University of Singapore Graduate Medical School, Singapore., Lodish H; Whitehead Institute for Biomedical Research, Cambridge, MA Department of Biology, Massachusetts Institute of Technology, Cambridge, MA., Sun L; Cardiovascular and Metabolic Disorders, Duke-National University of Singapore Graduate Medical School, Singapore Institute of Molecular and Cell Biology, Singapore sun.lei@duke-nus.edu.sg.
المصدر: Diabetes [Diabetes] 2014 Dec; Vol. 63 (12), pp. 4045-56. Date of Electronic Publication: 2014 Jul 09.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: American Diabetes Association Country of Publication: United States NLM ID: 0372763 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1939-327X (Electronic) Linking ISSN: 00121797 NLM ISO Abbreviation: Diabetes Subsets: MEDLINE
أسماء مطبوعة: Publication: Alexandria, VA : American Diabetes Association
Original Publication: [New York, American Diabetes Association]
مواضيع طبية MeSH: Adipocytes, Brown/*metabolism , Adipose Tissue, Brown/*metabolism , Cell Differentiation/*genetics , MicroRNAs/*physiology , RNA, Messenger/*analysis, Adipocytes, White/metabolism ; Animals ; Cells, Cultured ; Gene Knockout Techniques ; Mice ; Mice, Knockout ; RNA-Binding Proteins/genetics
مستخلص: Brown adipose tissue (BAT) is specialized to burn lipids for heat generation as a natural defense against cold and obesity. Previous studies established microRNAs (miRNAs) as essential regulators of brown adipocyte differentiation, but whether miRNAs are required for the feature maintenance of mature brown adipocytes remains unknown. To address this question, we ablated Dgcr8, a key regulator of the miRNA biogenesis pathway, in mature brown as well as in white adipocytes. Adipose tissue-specific Dgcr8 knockout mice displayed enlarged but pale interscapular brown fat with decreased expression of genes characteristic of brown fat and were intolerant to cold exposure. Primary brown adipocyte cultures in vitro confirmed that miRNAs are required for marker gene expression in mature brown adipocytes. We also demonstrated that miRNAs are essential for the browning of subcutaneous white adipocytes in vitro and in vivo. Using this animal model, we performed miRNA expression profiling analysis and identified a set of BAT-specific miRNAs that are upregulated during brown adipocyte differentiation and enriched in brown fat compared with other organs. We identified miR-182 and miR-203 as new regulators of brown adipocyte development. Taken together, our study demonstrates an essential role of miRNAs in the maintenance as well as in the differentiation of brown adipocytes.
(© 2014 by the American Diabetes Association. Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered.)
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معلومات مُعتمدة: T32 HL007627 United States HL NHLBI NIH HHS; R37 DK047618 United States DK NIDDK NIH HHS; R56 DK068348 United States DK NIDDK NIH HHS; R01 DK068348 United States DK NIDDK NIH HHS; K08-GM-102718 United States GM NIGMS NIH HHS; K08 GM102718 United States GM NIGMS NIH HHS; DK-068348-07 United States DK NIDDK NIH HHS; T32-HL-007627 United States HL NHLBI NIH HHS; DK-047618-25 United States DK NIDDK NIH HHS; R01 DK047618 United States DK NIDDK NIH HHS
المشرفين على المادة: 0 (Dgcr8 protein, mouse)
0 (MicroRNAs)
0 (RNA, Messenger)
0 (RNA-Binding Proteins)
تواريخ الأحداث: Date Created: 20140711 Date Completed: 20150116 Latest Revision: 20211021
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC4238002
DOI: 10.2337/db14-0466
PMID: 25008181
قاعدة البيانات: MEDLINE
الوصف
تدمد:1939-327X
DOI:10.2337/db14-0466