دورية أكاديمية
[Development of barcode and proteome profiling of glioblastoma].
| العنوان: | [Development of barcode and proteome profiling of glioblastoma]. |
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| المؤلفون: | Naryzhnyĭ SN, Ronzhina NL, Maĭnskova MA, Beliakova NV, Pantina RA, Filatov MV |
| المصدر: | Biomeditsinskaia khimiia [Biomed Khim] 2014 May-Jun; Vol. 60 (3), pp. 308-21. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | Russian |
| بيانات الدورية: | Publisher: Rossiĭskai︠a︡ akademii︠a︡ medit︠s︡inskikh nauk Country of Publication: Russia (Federation) NLM ID: 101196966 Publication Model: Print Cited Medium: Print ISSN: 2310-6972 (Print) Linking ISSN: 23106905 NLM ISO Abbreviation: Biomed Khim Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Moskva : Rossiĭskai︠a︡ akademii︠a︡ medit︠s︡inskikh nauk, 2003- |
| مواضيع طبية MeSH: | Gene Expression Regulation, Neoplastic*, Biomarkers, Tumor/*classification , Biomarkers, Tumor/*genetics , Brain Neoplasms/*genetics , Glioblastoma/*genetics , Tumor Suppressor Protein p53/*genetics, Annexin A1/genetics ; Annexin A1/metabolism ; Biomarkers, Tumor/metabolism ; Brain Neoplasms/diagnosis ; Brain Neoplasms/metabolism ; Cell Line, Tumor ; Cofilin 1/genetics ; Cofilin 1/metabolism ; Electrophoresis, Gel, Two-Dimensional ; Gene Expression Profiling ; Glioblastoma/diagnosis ; Glioblastoma/metabolism ; Humans ; Mass Spectrometry ; Molecular Sequence Annotation ; Molecular Typing ; Phosphopyruvate Hydratase/genetics ; Phosphopyruvate Hydratase/metabolism ; Proliferating Cell Nuclear Antigen/genetics ; Proliferating Cell Nuclear Antigen/metabolism ; Protein Isoforms/genetics ; Protein Isoforms/metabolism ; Proteome ; Pyruvate Kinase/genetics ; Pyruvate Kinase/metabolism ; Tumor Protein, Translationally-Controlled 1 ; Tumor Suppressor Protein p53/metabolism |
| مستخلص: | High grade glioma (glioblastoma) is the most common brain tumor. Its malignancy makes it the fourth biggest cause of cancer death. In our experiments we used several glioblastoma cell lines generated in our laboratory to obtain proteomics information specific for this disease. This study starts our developing the complete 2DE map of glioblastoma proteins. 2DE separation with following imaging, immunochemistry, spot picking, and mass-spectrometry allowed us detecting and identifying more than 100 proteins. Several of them have prominent differences in their level between norm and cancer. Among them are alpha-enolase (ENOA_HUMAN), pyruvate kinase isozymes M1/M2 (KPYM_HUMAN), cofilin 1 (COF1_HUMAN), translationally-controlled tumor protein TCTP_HUMAN, annexin 1 (ANXA1_HUMAN), PCNA (PCNA_HUMAN), p53 (TP53_HUMAN) and others. Most interesting results were obtained with protein p53. In all glioblastoma cell lines, its level was dramatically up regulated and enriched by multiple additional isoforms. This distribution is well correlated with presence of these proteins inside of cells themselves. At this initial step we suggest the panel of specific brain tumor markers (signature) to help creating noninvasive techniques to diagnose disease. These preliminary data point to these proteins as promising markers of glioblastoma. |
| فهرسة مساهمة: | Keywords: PCNA; glioblastoma; p53; proteome Local Abstract: [Publisher, Russian] Glioblastoma iavliaetsia naibolee chastoĭ opukhol'iu mozga. Po smertnosti ona zanimaet 4 mesto sredi rakovykh zabolevaniĭ. V nashikh éksperimentakh my ispol'zovali neskol'ko glioblastomnykh kletochnykh liniĭ dlia polucheniia proteomnoĭ informatsii, kharakternoĭ dlia dannogo zabolevaniia. Razdelenie belkov dvumernym élektroforezom s posleduiushchim okrashivaniem, analizom izobrazheniĭ i belkovykh piaten, mass-spektrometricheskoĭ i immunologicheskoĭ identifikatsieĭ belkov, pozvolilo nam vizualizirovat' bolee 600 belkovykh piaten i identifitsirovat' bolee 130 iz nikh. Proteomnye profili v normal'nykh i glioblastomnykh kletkakh ochen' pokhozhi, odnako urovni soderzhaniia mnogikh belkov sil'no otlichaiutsia. Sredi takikh belkov – al'fa-enolaza (ENOA_HUMAN), izoferment piruvatkinazy M1/M2 (KPYM_HUMAN), kofilin 1 (COF1_HUMAN), opukholevyĭ belok TCTP (TCTP_HUMAN), anneksin 1 (ANXA1_HUMAN), anneksin 2 (ANXA2_HUMAN), PCNA (PCNA_HUMAN), p53 (TP53_HUMAN) i drugie. Samye interesnye rezul'taty byli polucheny dlia belka r53. Po sravneniiu s normal'nymi kletkami v glioblastomnykh kletkakh uroven' dannogo belka ne tol'ko sil'no povyshen, no i eshche i soprovozhdaetsia poiavleniem mnozhestva dopolnitel'nykh form. Ispol'zovanie immunofermentnogo analiza (Vestern-blota) trekh khab-belkov (r53, 14-3-3 i PCNA) pozvolilo poluchit' minimal'nyĭ shtrikh-kod glioblastomnykh liniĭ. Ispol'zovanie takogo shtrikh-koda dast vozmozhnost' provedeniia diagnostiki s ispol'zovaniem biologicheskikh zhidkosteĭ patsientov. |
| المشرفين على المادة: | 0 (Annexin A1) 0 (Biomarkers, Tumor) 0 (CFL1 protein, human) 0 (Cofilin 1) 0 (Proliferating Cell Nuclear Antigen) 0 (Protein Isoforms) 0 (Proteome) 0 (TPT1 protein, human) 0 (Tumor Protein, Translationally-Controlled 1) 0 (Tumor Suppressor Protein p53) EC 2.7.1.40 (Pyruvate Kinase) EC 4.2.1.11 (Phosphopyruvate Hydratase) |
| تواريخ الأحداث: | Date Created: 20140715 Date Completed: 20140820 Latest Revision: 20211203 |
| رمز التحديث: | 20231215 |
| DOI: | 10.18097/pbmc20146003308 |
| PMID: | 25019393 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2310-6972 |
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| DOI: | 10.18097/pbmc20146003308 |