دورية أكاديمية
Uroguanylin inhibits H-ATPase activity and surface expression in renal distal tubules by a PKG-dependent pathway.
| العنوان: | Uroguanylin inhibits H-ATPase activity and surface expression in renal distal tubules by a PKG-dependent pathway. |
|---|---|
| المؤلفون: | da Silva Lima V; Superior Institute of Biomedical Sciences, State University of Ceará, Fortaleza, Ceará, Brazil;, Crajoinas RO; Heart Institute (InCor), and., Carraro-Lacroix LR; Department of Physiology and Biophysics, Biomedical Sciences Institute, University of São Paulo, São Paulo, São Paulo, Brazil;, Godinho AN; Superior Institute of Biomedical Sciences, State University of Ceará, Fortaleza, Ceará, Brazil;, Dias JL; Superior Institute of Biomedical Sciences, State University of Ceará, Fortaleza, Ceará, Brazil;, Dariolli R; Heart Institute (InCor), and., Girardi AC; Heart Institute (InCor), and., Fonteles MC; Superior Institute of Biomedical Sciences, State University of Ceará, Fortaleza, Ceará, Brazil; Mackenzie University, São Paulo, São Paulo, Brazil., Malnic G; Department of Physiology and Biophysics, Biomedical Sciences Institute, University of São Paulo, São Paulo, São Paulo, Brazil;, Lessa LM; Superior Institute of Biomedical Sciences, State University of Ceará, Fortaleza, Ceará, Brazil; lucilialessa17@gmail.com. |
| المصدر: | American journal of physiology. Cell physiology [Am J Physiol Cell Physiol] 2014 Sep 15; Vol. 307 (6), pp. C532-41. Date of Electronic Publication: 2014 Jul 16. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Physiological Society Country of Publication: United States NLM ID: 100901225 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-1563 (Electronic) Linking ISSN: 03636143 NLM ISO Abbreviation: Am J Physiol Cell Physiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Bethesda, Md. : American Physiological Society, |
| مواضيع طبية MeSH: | Cell Membrane/*drug effects , Cyclic GMP-Dependent Protein Kinases/*metabolism , Kidney Tubules, Distal/*drug effects , Natriuretic Peptides/*pharmacology , Proton-Translocating ATPases/*metabolism, Animals ; Bicarbonates/metabolism ; Cell Membrane/enzymology ; Cyclic GMP/metabolism ; Cyclic GMP-Dependent Protein Kinases/antagonists & inhibitors ; Dogs ; Hydrogen-Ion Concentration ; Kidney Tubules, Distal/enzymology ; Madin Darby Canine Kidney Cells ; Male ; Perfusion ; Protein Kinase Inhibitors/pharmacology ; Protein Transport ; Rats ; Rats, Wistar ; Receptors, Guanylate Cyclase-Coupled/drug effects ; Receptors, Guanylate Cyclase-Coupled/genetics ; Receptors, Guanylate Cyclase-Coupled/metabolism ; Signal Transduction/drug effects ; Time Factors |
| مستخلص: | Cumulative evidence suggests that guanylin peptides play an important role on electrolyte homeostasis. We have previously reported that uroguanylin (UGN) inhibits bicarbonate reabsorption in a renal distal tubule. In the present study, we tested the hypothesis that the bicarbonaturic effect of UGN is at least in part attributable to inhibition of H(+)-ATPase-mediated hydrogen secretion in the distal nephron. By in vivo stationary microperfusion experiments, we were able to show that UGN inhibits H(+)-ATPase activity by a PKG-dependent pathway because KT5823 (PKG inhibitor) abolished the UGN effect on distal bicarbonate reabsorption and H89 (PKA inhibitor) was unable to prevent it. The in vivo results were confirmed by the in vitro experiments, where we used fluorescence microscopy to measure intracellular pH (pHi) recovery after an acid pulse with NH4Cl. By this technique, we observed that UGN and 8 bromoguanosine-cGMP (8Br-cGMP) inhibited H(+)-ATPase-dependent pHi recovery and that the UGN inhibitory effect was abolished in the presence of the PKG inhibitor. In addition, by using RT-PCR technique, we verified that Madin-Darby canine kidney (MDCK)-C11 cells express guanylate cyclase-C. Besides, UGN stimulated an increase of both cGMP content and PKG activity but was unable to increase the production of cellular cAMP content and PKA activity. Furthermore, we found that UGN reduced cell surface abundance of H+-ATPase B1 subunit in MDCK-C11 and that this effect was abolished by the PKG inhibitor. Taken together, our data suggest that UGN inhibits H(+)-ATPase activity and surface expression in renal distal cells by a cGMP/PKG-dependent pathway. (Copyright © 2014 the American Physiological Society.) |
| References: | J Clin Invest. 2013 Oct;123(10):4219-31. (PMID: 24051376) Clin Sci (Lond). 2012 Dec;123(12):659-68. (PMID: 22877138) Can J Physiol Pharmacol. 2011 Aug;89(8):575-85. (PMID: 21815750) J Biol Chem. 2002 May 17;277(20):17758-64. (PMID: 11889121) Regul Pept. 2009 Nov 27;158(1-3):6-13. (PMID: 19632278) J Nephrol. 2010 Nov-Dec;23 Suppl 16:S19-27. (PMID: 21170879) J Clin Invest. 1976 Aug;58(2):454-69. (PMID: 783200) Am J Physiol Renal Physiol. 2000 Feb;278(2):F180-91. (PMID: 10662722) J Clin Invest. 1971 Mar;50(3):667-78. (PMID: 5101785) Pflugers Arch. 2009 Sep;458(5):969-79. (PMID: 19396617) Pflugers Arch. 2009 May;458(1):137-56. (PMID: 19277700) Histochem Cell Biol. 2001 Feb;115(2):131-45. (PMID: 11444148) Am J Physiol Renal Physiol. 2010 Dec;299(6):F1433-42. (PMID: 20861080) J Biol Chem. 1951 Nov;193(1):265-75. (PMID: 14907713) Am J Physiol Renal Physiol. 2012 Mar 1;302(5):F614-24. (PMID: 22129966) Nat Rev Mol Cell Biol. 2007 Nov;8(11):917-29. (PMID: 17912264) Pflugers Arch. 1989 Jan;413(3):280-6. (PMID: 2717375) Nat Genet. 1999 Jan;21(1):84-90. (PMID: 9916796) Kidney Int. 2005 Sep;68(3):1008-17. (PMID: 16105031) Pflugers Arch. 2000 Dec;441(2-3):287-93. (PMID: 11211115) Kidney Int. 2004 Jan;65(1):40-53. (PMID: 14675035) J Biol Chem. 1999 May 21;274(21):14670-7. (PMID: 10329661) J Physiol. 1997 Dec 1;505 ( Pt 2):411-23. (PMID: 9423183) Arch Biochem Biophys. 1999 Jul 1;367(1):137-9. (PMID: 10375409) Pflugers Arch. 1994 Sep;428(2):157-62. (PMID: 7971172) Physiol Rev. 2004 Oct;84(4):1263-314. (PMID: 15383652) Biochemistry. 1979 May 29;18(11):2210-8. (PMID: 36128) J Am Soc Nephrol. 2008 Jan;19(1):84-91. (PMID: 18178800) Am J Physiol Renal Physiol. 2012 Nov 15;303(10):F1399-408. (PMID: 22952280) J Biol Chem. 2000 Oct 13;275(41):32331-7. (PMID: 10915794) Can J Physiol Pharmacol. 2006 Oct;84(10):1003-10. (PMID: 17218966) Endocrinology. 2001 Jul;142(7):3087-97. (PMID: 11416031) Regul Pept. 2009 Oct 9;157(1-3):37-43. (PMID: 19540271) Am J Physiol Cell Physiol. 2011 Nov;301(5):C1251-61. (PMID: 21832245) Annu Rev Physiol. 2008;70:379-403. (PMID: 17988205) J Gen Physiol. 1976 Jan;67(1):91-112. (PMID: 1460) Pancreas. 2002 Aug;25(2):170-5. (PMID: 12142741) Am J Physiol. 1994 Oct;267(4 Pt 2):F509-15. (PMID: 7524355) Kidney Int. 2002 Nov;62(5):1693-9. (PMID: 12371969) |
| فهرسة مساهمة: | Keywords: H+-ATPase; PKG; cGMP; distal tubule; renal microperfusion; uroguanylin |
| المشرفين على المادة: | 0 (Bicarbonates) 0 (Natriuretic Peptides) 0 (Protein Kinase Inhibitors) 152175-68-3 (uroguanylin) EC 2.7.11.12 (Cyclic GMP-Dependent Protein Kinases) EC 3.6.3.14 (Proton-Translocating ATPases) EC 4.6.1.2 (Receptors, Guanylate Cyclase-Coupled) H2D2X058MU (Cyclic GMP) |
| تواريخ الأحداث: | Date Created: 20140718 Date Completed: 20141106 Latest Revision: 20211021 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC4166739 |
| DOI: | 10.1152/ajpcell.00392.2013 |
| PMID: | 25031022 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1522-1563 |
|---|---|
| DOI: | 10.1152/ajpcell.00392.2013 |