دورية أكاديمية
أعرض في EDS

DNA methylation analysis in self-sampled brush material as a triage test in hrHPV-positive women.

التفاصيل البيبلوغرافية
العنوان: DNA methylation analysis in self-sampled brush material as a triage test in hrHPV-positive women.
المؤلفون: Boers A; Department of Gynaecologic Oncology, Cancer Research Center, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands., Bosgraaf RP; Department of Obstetrics and Gynaecology, Radboud University Medical Center, Nijmegen, The Netherlands., van Leeuwen RW; Department of Gynaecologic Oncology, Cancer Research Center, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands., Schuuring E; Department of Pathology, Cancer Research Center, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands., Heideman DA; Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands., Massuger LF; Department of Obstetrics and Gynaecology, Radboud University Medical Center, Nijmegen, The Netherlands., Verhoef VM; Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands., Bulten J; Department of Pathology, Radboud University Medical Center, Nijmegen, The Netherlands., Melchers WJ; Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, The Netherlands., van der Zee AG; Department of Gynaecologic Oncology, Cancer Research Center, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands., Bekkers RL; Department of Obstetrics and Gynaecology, Radboud University Medical Center, Nijmegen, The Netherlands., Wisman GB; Department of Gynaecologic Oncology, Cancer Research Center, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
المصدر: British journal of cancer [Br J Cancer] 2014 Sep 09; Vol. 111 (6), pp. 1095-101. Date of Electronic Publication: 2014 Jul 17.
نوع المنشور: Comparative Study; Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group on behalf of Cancer Research UK Country of Publication: England NLM ID: 0370635 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-1827 (Electronic) Linking ISSN: 00070920 NLM ISO Abbreviation: Br J Cancer Subsets: MEDLINE
أسماء مطبوعة: Publication: 2002- : London : Nature Publishing Group on behalf of Cancer Research UK
Original Publication: London, Lewis.
مواضيع طبية MeSH: DNA Methylation*, Papillomavirus Infections/*virology , Triage/*methods , Uterine Cervical Neoplasms/*diagnosis , Vaginal Smears/*methods , Uterine Cervical Dysplasia/*diagnosis, Adult ; Cell Adhesion Molecules/genetics ; Early Detection of Cancer/methods ; Female ; Humans ; Microfilament Proteins/genetics ; Middle Aged ; Patient Compliance ; Risk Factors ; Self Care ; Sensitivity and Specificity ; Specimen Handling ; Telomerase/genetics ; Tumor Suppressor Proteins/genetics ; Uterine Cervical Neoplasms/virology ; Uterine Cervical Dysplasia/virology
مستخلص: Background: Primary high-risk human papillomavirus (hrHPV) testing in cervical cancer screening shows relatively low specificity, which makes triage testing necessary. In this study, DNA methylation analysis was compared with cytology for triage testing in hrHPV-positive women. Moreover, feasibility of DNA methylation analysis directly on brush-based self-sampled specimens was assessed.
Methods: Non-responding women from population-based screening were invited to self-collect a cervico-vaginal specimen for hrHPV testing; hrHPV-positive women were referred to a physician for triage liquid-based cytology. DNA methylation analysis was performed on 128 hrHPV-positive physician-collected triage samples and 50 matched brush self-samples with QMSP for C13ORF18, EPB41L3, JAM3 and TERT.
Results: In physician-taken triage material, DNA methylation analysis of JAM3 showed the highest combined specificity (88%) and sensitivity (82%) for detection of CIN3+, whereas cytology showed a specificity of 48% and a sensitivity of 91%. Out of 39 women with abnormal cytology and normal histology (false-positive by cytology), 87% were negative for JAM3 and 90% for C13ORF18 methylation. Agreement between DNA methylation analysis performed directly on the matched self-sampled material and physician-taken samples was 88% for JAM3 (κ=0.75, P<0.001) and 90% for C13ORF18 (κ=0.77; P<0.001).
Conclusions: DNA methylation analysis as a triage test in hrHPV-positive women is an attractive alternative to cytology. Furthermore, DNA methylation is feasible directly on brush-based self-samplers and showed good correlation with matched physician-taken samples. Direct molecular triage on self-collected specimens could optimise the screening program, especially for non-responders, as this would eliminate the need for an additional physician-taken scraping for triage testing.
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المشرفين على المادة: 0 (Cell Adhesion Molecules)
0 (EPB41L3 protein, human)
0 (JAM3 protein, human)
0 (Microfilament Proteins)
0 (RUBCNL protein, human)
0 (Tumor Suppressor Proteins)
EC 2.7.7.49 (TERT protein, human)
EC 2.7.7.49 (Telomerase)
تواريخ الأحداث: Date Created: 20140718 Date Completed: 20141104 Latest Revision: 20221207
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC4453842
DOI: 10.1038/bjc.2014.392
PMID: 25032730
قاعدة البيانات: MEDLINE
الوصف
تدمد:1532-1827
DOI:10.1038/bjc.2014.392