دورية أكاديمية
Identification of peptides that selectively bind to myoglobin by biopanning of phage displayed-peptide library.
| العنوان: | Identification of peptides that selectively bind to myoglobin by biopanning of phage displayed-peptide library. |
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| المؤلفون: | Padmanaban G; Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea; Cell & Matrix Research Institute, Kyungpook National University, Daegu, Republic of Korea; BK21 Plus KNU Biomedical Convergence Program, Department of Biomedical Science, Kyungpook National University, Daegu, Republic of Korea., Park H; Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea., Choi JS; Department of Chemical Engineering, Hanyang University, Gyeonggi-do, Republic of Korea., Cho YW; Department of Chemical Engineering, Hanyang University, Gyeonggi-do, Republic of Korea., Kang WC; Department of Cardiology, Gil Medical Center, Gachon University of Medicine and Science, Incheon, Republic of Korea., Moon CI; Department of Cardiology, School of Medicine, Hanyang University, Seoul, Republic of Korea., Kim IS; Biomedical Research Institute, KIST, Seoul, Republic of Korea., Lee BH; Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea; Cell & Matrix Research Institute, Kyungpook National University, Daegu, Republic of Korea; BK21 Plus KNU Biomedical Convergence Program, Department of Biomedical Science, Kyungpook National University, Daegu, Republic of Korea. Electronic address: leebh@knu.ac.kr. |
| المصدر: | Journal of biotechnology [J Biotechnol] 2014 Oct 10; Vol. 187, pp. 43-50. Date of Electronic Publication: 2014 Jul 29. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Publishers Country of Publication: Netherlands NLM ID: 8411927 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4863 (Electronic) Linking ISSN: 01681656 NLM ISO Abbreviation: J Biotechnol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam : Elsevier Science Publishers, c1984- |
| مواضيع طبية MeSH: | Peptide Library*, Biomarkers/*blood , Enzyme-Linked Immunosorbent Assay/*methods , Myoglobin/*blood , Peptides/*metabolism, Biomarkers/metabolism ; Heart Diseases/blood ; Humans ; Myoglobin/metabolism ; Peptides/chemistry ; Peptides/isolation & purification ; Protein Binding ; Reproducibility of Results |
| مستخلص: | Biopanning of phage displayed-peptide library was performed against myoglobin, a marker for the early assessment of acute myocardial infarction (AMI), to identify peptides that selectively bind to myoglobin. Using myoglobin-conjugated magnetic beads, phages that bound to myoglobin were collected and amplified for the next round of screening. A 148-fold enrichment of phage titer was observed after five rounds of screening relative to the first round. After phage binding ELISA, three phage clones were selected (3R1, 3R7 and 3R10) and the inserted peptides were chemically synthesized. The analysis of binding affinity showed that the 3R7 (CPSTLGASC) peptide had higher binding affinity (Kd=57 nM) than did the 3R1 (CNLSSSWIC) and 3R10 (CVPRLSAPC) peptide (Kd=125 nM and 293 nM, respectively). Cross binding activity to other proteins, such as bovine serum albumin, troponin I, and creatine kinase-MB, was minimal. In a peptide-antibody sandwich ELISA, the selected peptides efficiently captured myoglobin. Moreover, the concentrations of myoglobin in serum samples measured by a peptide-peptide sandwich assay were comparable to those measured by a commercial antibody-based kit. These results indicate that the identified peptides can be used for the detection of myoglobin and may be a cost effective alternative to antibodies. (Copyright © 2014 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Cardiac biomarkers; Myoglobin; Peptide; Phage display; Sandwich assays |
| المشرفين على المادة: | 0 (Biomarkers) 0 (Myoglobin) 0 (Peptide Library) 0 (Peptides) |
| تواريخ الأحداث: | Date Created: 20140801 Date Completed: 20150623 Latest Revision: 20151119 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.jbiotec.2014.07.435 |
| PMID: | 25078431 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-4863 |
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| DOI: | 10.1016/j.jbiotec.2014.07.435 |