دورية أكاديمية
In vitro Scleroderma laeve and Eucalyptus grandis mycorrhization and analysis of atp6, 17S rDNA, and ras gene expression during ectomycorrhizal formation.
| العنوان: | In vitro Scleroderma laeve and Eucalyptus grandis mycorrhization and analysis of atp6, 17S rDNA, and ras gene expression during ectomycorrhizal formation. |
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| المؤلفون: | de Freitas Pereira M; Department of Microbiology/BIOAGRO, Federal University of Viçosa, Viçosa-Minas Gerais (MG), Brazil., Betancourth BM, Teixeira JA, Zubieta MP, de Queiroz MV, Kasuya MC, Costa MD, de Araújo EF |
| المصدر: | Journal of basic microbiology [J Basic Microbiol] 2014 Dec; Vol. 54 (12), pp. 1358-66. Date of Electronic Publication: 2014 Jul 30. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-VCH Verlag Country of Publication: Germany NLM ID: 8503885 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1521-4028 (Electronic) Linking ISSN: 0233111X NLM ISO Abbreviation: J Basic Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2005->: Weinheim, Germany : Wiley-VCH Verlag Original Publication: Berlin : Akademie-Verlag, [1985- |
| مواضيع طبية MeSH: | Genes, Fungal* , Genes, ras*, Basidiomycota/*metabolism , DNA, Ribosomal/*metabolism , Eucalyptus/*metabolism , Fungal Proteins/*metabolism , Mycorrhizae/*metabolism, Basidiomycota/genetics ; DNA, Ribosomal/genetics ; Eucalyptus/genetics ; Fungal Proteins/genetics ; Mycorrhizae/genetics ; Mycorrhizae/growth & development ; Signal Transduction |
| مستخلص: | The interaction between fungi and plants that form ectomycorrhizae (ECM) promotes alterations in the gene expression profiles of both organisms. Fungal genes expression related to metabolism were evaluated at the pre-symbiotic stage and during the ECM development between Scleroderma laeve and Eucalyptus grandis. Partial sequences of ATP synthase (atp6), translation elongation factor (ef1α), the RAS protein (ras), and the 17S rDNA genes were isolated. The expression of the atp6 and 17S rDNA genes during the pre-symbiotic stage showed an approximately threefold increase compared to the control. During ECM development, the expression of the 17S rDNA gene showed a 4.4-fold increase after 3 days of contact, while the expression of the atp6 gene increased 7.23-fold by the 15th day, suggesting that protein synthesis and respiratory chain activities are increased during the formation of the mantle and the Hartig net. The ras gene transcripts were only detected by RT-PCR 30 days after fungus-plant contact, suggesting that RAS-mediated signal transduction pathways are functional during the establishment of symbiosis. The present study demonstrates that alterations in gene expression occur in response to stimuli released by the plant during ECM association and increases the understanding of the association between S. laeve and E. grandis. (© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.) |
| فهرسة مساهمة: | Keywords: Ectomycorrhizae; Eucalyptus grandis; In vitro mycorrhization; Scleroderma laeve; Symbiosis-regulated genes |
| المشرفين على المادة: | 0 (DNA, Ribosomal) 0 (Fungal Proteins) |
| تواريخ الأحداث: | Date Created: 20140801 Date Completed: 20150723 Latest Revision: 20141216 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1002/jobm.201400253 |
| PMID: | 25080195 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1521-4028 |
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| DOI: | 10.1002/jobm.201400253 |