دورية أكاديمية
The FACT complex interacts with the E3 ubiquitin ligase Psh1 to prevent ectopic localization of CENP-A.
| العنوان: | The FACT complex interacts with the E3 ubiquitin ligase Psh1 to prevent ectopic localization of CENP-A. |
|---|---|
| المؤلفون: | Deyter GM; Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA., Biggins S; Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA sbiggins@fhcrc.org. |
| المصدر: | Genes & development [Genes Dev] 2014 Aug 15; Vol. 28 (16), pp. 1815-26. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cold Spring Harbor Laboratory Press Country of Publication: United States NLM ID: 8711660 Publication Model: Print Cited Medium: Internet ISSN: 1549-5477 (Electronic) Linking ISSN: 08909369 NLM ISO Abbreviation: Genes Dev Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cold Spring Harbor, NY : Cold Spring Harbor Laboratory Press Original Publication: [Cold Spring Harbor, N.Y.] : Cold Spring Harbor Laboratory in association with the Genetical Society of Great Britain, [c1987- |
| مواضيع طبية MeSH: | Autoantigens/*metabolism , Chromosomal Proteins, Non-Histone/*metabolism , DNA-Binding Proteins/*metabolism , High Mobility Group Proteins/*metabolism , Peptide Elongation Factors/*metabolism , Saccharomyces cerevisiae/*physiology , Saccharomyces cerevisiae Proteins/*metabolism , Transcriptional Elongation Factors/*metabolism , Ubiquitin-Protein Ligases/*metabolism, Centromere Protein A ; Euchromatin/metabolism ; Mutation ; Nucleosomes/metabolism ; Peptide Elongation Factors/genetics ; Protein Binding ; Protein Stability ; Protein Transport ; Proteolysis ; Saccharomyces cerevisiae/enzymology ; Ubiquitin-Protein Ligases/genetics ; Ubiquitination |
| مستخلص: | Centromere identity and its epigenetic maintenance require the incorporation of a histone H3 variant called CENP-A at centromeres. CENP-A mislocalization to ectopic sites may disrupt chromatin-based processes and chromosome segregation, so it is important to uncover the mechanisms by which this variant is exclusively localized to centromeres. Here, we identify a role for the conserved chromatin-modifying complex FACT (facilitates chromatin transcription/transactions) in preventing budding yeast CENP-A(Cse4) mislocalization to euchromatin by mediating its proteolysis. The Spt16 subunit of the FACT complex binds to Psh1 (Pob3/Spt16/histone), an E3 ubiquitin ligase that targets CENP-A(Cse4) for degradation. The interaction between Psh1 and Spt16 is critical for both CENP-A(Cse4) ubiquitylation and its exclusion from euchromatin. We found that Psh1 cannot efficiently ubiquitylate CENP-A(Cse4) nucleosomes in vitro, suggesting that additional factors must facilitate CENP-A(Cse4) removal from chromatin in vivo. Consistent with this, a Psh1 mutant that cannot associate with FACT has a reduced interaction with CENP-A(Cse4) in vivo. Together, our data identify a previously unknown mechanism to maintain centromere identity and genomic stability through the FACT-mediated degradation of ectopically localized CENP-A(Cse4). (© 2014 Deyter and Biggins; Published by Cold Spring Harbor Laboratory Press.) |
| References: | Mol Cell. 2007 Jun 22;26(6):853-65. (PMID: 17569568) EMBO J. 2011 May 18;30(10):1919-27. (PMID: 21505420) Mol Cell. 2013 Aug 22;51(4):469-79. (PMID: 23973375) Nucleic Acids Res. 2005 May 20;33(9):2868-79. (PMID: 15908586) Genetics. 2013 Jun;194(2):513-8. (PMID: 23525333) Science. 2003 Aug 22;301(5636):1096-9. (PMID: 12934008) Nature. 2004 Jul 29;430(6999):578-82. (PMID: 15282608) Mol Cell. 2009 Aug 14;35(3):365-76. (PMID: 19683499) Genetics. 2011 Jan;187(1):9-19. (PMID: 20944015) Mol Cell. 2009 Sep 24;35(6):794-805. (PMID: 19782029) Curr Opin Cell Biol. 2013 Jun;25(3):334-40. (PMID: 23490282) Nature. 2006 Jan 26;439(7075):497-501. (PMID: 16299494) J Biol Chem. 2006 Aug 18;281(33):23297-301. (PMID: 16766522) Curr Biol. 2011 Sep 13;21(17):1488-93. (PMID: 21871803) Mol Cell Biol. 2001 Mar;21(6):2098-106. (PMID: 11238944) Curr Biol. 2004 Nov 9;14(21):1968-72. (PMID: 15530401) Yeast. 1998 Jul;14(10):953-61. (PMID: 9717241) Nucleic Acids Res. 2004 Nov 01;32(19):5894-906. (PMID: 15520471) Cell. 1998 Jan 9;92(1):105-16. (PMID: 9489704) BMC Genomics. 2009 Jan 21;10:37. (PMID: 19159457) Genetics. 2013 Aug;194(4):817-46. (PMID: 23908374) Mol Cell Biol. 2002 Oct;22(20):6979-92. (PMID: 12242279) Cell. 2009 May 1;137(3):472-84. (PMID: 19410544) J Biol Chem. 2013 Apr 12;288(15):10188-94. (PMID: 23417676) J Biol Chem. 2009 Aug 28;284(35):23461-71. (PMID: 19574230) Mol Cell. 2010 Nov 12;40(3):444-54. (PMID: 21070970) Nature. 2013 Jul 4;499(7456):111-4. (PMID: 23698368) Science. 2013 May 31;340(6136):1110-3. (PMID: 23723239) Nat Commun. 2011;2:313. (PMID: 21587230) Cancer Res. 2003 Jul 1;63(13):3511-6. (PMID: 12839935) Nature. 2011 Jul 10;476(7359):232-5. (PMID: 21743476) Genetics. 2002 Dec;162(4):1557-71. (PMID: 12524332) Proc Natl Acad Sci U S A. 2012 Jan 3;109(1):243-8. (PMID: 22184235) Biochemistry. 1978 Nov 14;17(23):4955-64. (PMID: 718868) EMBO J. 2001 Jul 2;20(13):3506-17. (PMID: 11432837) Mol Cell. 2009 May 14;34(4):405-15. (PMID: 19481521) PLoS Genet. 2013;9(2):e1003317. (PMID: 23468649) Proc Natl Acad Sci U S A. 2011 Oct 4;108(40):16588-93. (PMID: 21949362) Genetics. 2014 Apr;196(4):1041-5. (PMID: 24514906) Genetics. 2000 Aug;155(4):1593-606. (PMID: 10924459) Mol Cell. 2014 Feb 20;53(4):631-44. (PMID: 24530302) Chromosome Res. 2013 Jul;21(4):407-18. (PMID: 23793898) Nature. 2011 Apr 14;472(7342):234-7. (PMID: 21412236) Mol Cell. 2009 Aug 14;35(3):377-83. (PMID: 19683500) J Biol Chem. 2011 Feb 4;286(5):4021-6. (PMID: 21115484) J Cell Biol. 2010 Jun 28;189(7):1143-55. (PMID: 20566683) Mol Cell. 2006 May 5;22(3):363-74. (PMID: 16678108) Nat Cell Biol. 2006 May;8(5):458-69. (PMID: 16622419) Science. 2003 Aug 22;301(5636):1090-3. (PMID: 12934006) Nat Methods. 2009 Dec;6(12):917-22. (PMID: 19915560) Genetics. 2008 May;179(1):263-75. (PMID: 18458100) Mol Cell Biol. 2005 Jul;25(14):5812-22. (PMID: 15987999) Mol Cell Biol. 2004 May;24(9):3907-17. (PMID: 15082784) Curr Biol. 2007 Jul 17;17(14):1219-24. (PMID: 17614284) J Biol Chem. 2011 Sep 2;286(35):30504-30512. (PMID: 21757688) Mol Biol Cell. 2009 Sep;20(18):3986-95. (PMID: 19625449) J Biol Chem. 2011 Dec 2;286(48):41883-41892. (PMID: 21969370) J Biol Chem. 1998 Aug 21;273(34):21972-9. (PMID: 9705338) Proc Natl Acad Sci U S A. 2008 Jul 1;105(26):8884-9. (PMID: 18579787) PLoS Genet. 2012 Sep;8(9):e1002985. (PMID: 23028377) Mol Cancer. 2009 Dec 10;8:119. (PMID: 20003272) Mol Cell. 2010 Nov 12;40(3):455-64. (PMID: 21070971) |
| معلومات مُعتمدة: | P30 CA015704 United States CA NCI NIH HHS; R01 GM078069 United States GM NIGMS NIH HHS; GM078069 United States GM NIGMS NIH HHS |
| فهرسة مساهمة: | Keywords: CENP-A; FACT; Psh1; centromere; proteolysis; ubiquitylation |
| المشرفين على المادة: | 0 (Autoantigens) 0 (Centromere Protein A) 0 (Chromosomal Proteins, Non-Histone) 0 (DNA-Binding Proteins) 0 (Euchromatin) 0 (FACT protein, S cerevisiae) 0 (High Mobility Group Proteins) 0 (Nucleosomes) 0 (Peptide Elongation Factors) 0 (SPT16 protein, S cerevisiae) 0 (Saccharomyces cerevisiae Proteins) 0 (Transcriptional Elongation Factors) EC 2.3.2.27 (Psh1 protein, S cerevisiae) EC 2.3.2.27 (Ubiquitin-Protein Ligases) |
| تواريخ الأحداث: | Date Created: 20140817 Date Completed: 20141001 Latest Revision: 20211021 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC4197964 |
| DOI: | 10.1101/gad.243113.114 |
| PMID: | 25128498 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1549-5477 |
|---|---|
| DOI: | 10.1101/gad.243113.114 |