دورية أكاديمية
أعرض في EDS

Immunization of Wistar female rats with 255-Gy-irradiated Toxoplasma gondii: preventing parasite load and maternofoetal transmission.

التفاصيل البيبلوغرافية
العنوان: Immunization of Wistar female rats with 255-Gy-irradiated Toxoplasma gondii: preventing parasite load and maternofoetal transmission.
المؤلفون: Camossi LG; Faculdade de Medicina Veterinária e Zootecnia, UNESP Univ Estadual Paulista, Campus de Botucatu, Departamento de Higiene Veterinária e Saúde Pública, Botucatu, SP, Brazil. Electronic address: lucilenecamossi@gmail.com., Fornazari F; Faculdade de Medicina Veterinária e Zootecnia, UNESP Univ Estadual Paulista, Campus de Botucatu, Departamento de Higiene Veterinária e Saúde Pública, Botucatu, SP, Brazil., Richini-Pereira VB; Faculdade de Medicina Veterinária e Zootecnia, UNESP Univ Estadual Paulista, Campus de Botucatu, Departamento de Higiene Veterinária e Saúde Pública, Botucatu, SP, Brazil., da Silva RC; Faculdade de Medicina Veterinária e Zootecnia, UNESP Univ Estadual Paulista, Campus de Botucatu, Departamento de Higiene Veterinária e Saúde Pública, Botucatu, SP, Brazil., Cardia DF; Faculdade de Medicina Veterinária e Zootecnia, UNESP Univ Estadual Paulista, Campus de Botucatu, Departamento de Higiene Veterinária e Saúde Pública, Botucatu, SP, Brazil., Langoni H; Faculdade de Medicina Veterinária e Zootecnia, UNESP Univ Estadual Paulista, Campus de Botucatu, Departamento de Higiene Veterinária e Saúde Pública, Botucatu, SP, Brazil. Electronic address: hlangoni@fmvz.unesp.br.
المصدر: Experimental parasitology [Exp Parasitol] 2014 Oct; Vol. 145, pp. 157-63. Date of Electronic Publication: 2014 Aug 25.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Academic Press Country of Publication: United States NLM ID: 0370713 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2449 (Electronic) Linking ISSN: 00144894 NLM ISO Abbreviation: Exp Parasitol Subsets: MEDLINE
أسماء مطبوعة: Publication: Orlando, FL : Academic Press
Original Publication: New York.
مواضيع طبية MeSH: Infectious Disease Transmission, Vertical/*prevention & control , Pregnancy Complications, Parasitic/*prevention & control , Toxoplasma/*immunology , Toxoplasmosis, Animal/*prevention & control, Animals ; Antibodies, Protozoan/blood ; Brain/parasitology ; Female ; Immunoglobulin G/blood ; Male ; Muscle, Skeletal/parasitology ; Parasite Load ; Peritoneal Cavity/parasitology ; Polymerase Chain Reaction ; Pregnancy ; Rats ; Rats, Wistar ; Real-Time Polymerase Chain Reaction ; Specific Pathogen-Free Organisms ; Toxoplasma/radiation effects ; Toxoplasmosis, Animal/transmission ; Viscera/parasitology
مستخلص: Toxoplasmosis, caused by an obligate intracellular protozoan parasite, Toxoplasma gondii, is an worldwide parasitic disease, with significant importance for animal production and considerable impact to the public health. This study was aimed to evaluate the dynamic of the distribution of T.gondii in tissues of female Wistar rats and their puppies tissues, after the immunization by oral rote with irradiated tachyzoites. One week after pregnancy confirmation, rats was challenged by gavage with T. gondii bradyzoites, oocysts or tachyzoites of T. gondii. Forty-eight pregnant rats were grouped as follow: immunized and challenged with bradyzoites (BZ*); non-immunized and challenged with bradyzoites (BZ); immunized and challenged with oocysts (OC*); non-immunized and challenged with oocysts (OC); immunized and challenged with tachyzoites (TZ*); non-immunized and challenged with tachyzoites (TZ); only immunized (I); control group (C). After parturition the rats were sacrificed and the tissues were researched for the DNA of T. gondii by polymerase chain reaction (PCR) and the parasite load determined by the quantitative PCR (qPCR). It was verified that the immunization with irradiated tachyzoites of T. gondii induced the reduction of parasitic load in most organs analyzed, although not prevent the establishment of infection with the parasite. And also, the immunization showed a favorable effect on the birth rate and litter size.
(Copyright © 2014 Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: Immunization; Parasite load; Toxoplasmosis; Wistar rats; Zoonosis
المشرفين على المادة: 0 (Antibodies, Protozoan)
0 (Immunoglobulin G)
تواريخ الأحداث: Date Created: 20140830 Date Completed: 20141214 Latest Revision: 20141003
رمز التحديث: 20231215
DOI: 10.1016/j.exppara.2014.08.012
PMID: 25169764
قاعدة البيانات: MEDLINE
الوصف
تدمد:1090-2449
DOI:10.1016/j.exppara.2014.08.012