Staphylococcus aureus alters growth activity, autolysis, and antibiotic tolerance in a human host-adapted Pseudomonas aeruginosa lineage.

التفاصيل البيبلوغرافية
العنوان:	Staphylococcus aureus alters growth activity, autolysis, and antibiotic tolerance in a human host-adapted Pseudomonas aeruginosa lineage.
المؤلفون:	Michelsen CF; Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark., Christensen AM; Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark., Bojer MS; Department of Veterinary Disease Biology, Food Safety and Zoonoses, University of Copenhagen, Frederiksberg, Denmark., Høiby N; Institute for International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark., Ingmer H; Department of Veterinary Disease Biology, Food Safety and Zoonoses, University of Copenhagen, Frederiksberg, Denmark., Jelsbak L; Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark lj@bio.dtu.dk.
المصدر:	Journal of bacteriology [J Bacteriol] 2014 Nov; Vol. 196 (22), pp. 3903-11. Date of Electronic Publication: 2014 Sep 02.
نوع المنشور:	Journal Article; Research Support, Non-U.S. Gov't
اللغة:	English
بيانات الدورية:	Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 2985120R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-5530 (Electronic) Linking ISSN: 00219193 NLM ISO Abbreviation: J Bacteriol Subsets: MEDLINE
أسماء مطبوعة:	Original Publication: Washington, DC : American Society for Microbiology
مواضيع طبية MeSH:	Anti-Bacterial Agents/pharmacology , Drug Resistance, Bacterial/physiology , Pseudomonas aeruginosa/drug effects , Pseudomonas aeruginosa/growth & development , Staphylococcus aureus/*physiology, Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Bacteriological Techniques ; Ciprofloxacin/pharmacology ; Coculture Techniques ; Gene Expression Regulation, Bacterial/physiology ; Gentamicins/pharmacology ; Host Specificity ; Humans ; Pseudomonas aeruginosa/classification ; Quorum Sensing
مستخلص:	Interactions among members of polymicrobial infections or between pathogens and the commensal flora may determine disease outcomes. Pseudomonas aeruginosa and Staphylococcus aureus are important opportunistic human pathogens and are both part of the polymicrobial infection communities in human hosts. In this study, we analyzed the in vitro interaction between S. aureus and a collection of P. aeruginosa isolates representing different evolutionary steps of a dominant lineage, DK2, that have evolved through decades of growth in chronically infected patients. While the early adapted P. aeruginosa DK2 strains outcompeted S. aureus during coculture on agar plates, we found that later P. aeruginosa DK2 strains showed a commensal-like interaction, where S. aureus was not inhibited by P. aeruginosa and the growth activity of P. aeruginosa was enhanced in the presence of S. aureus. This effect is mediated by one or more extracellular S. aureus proteins greater than 10 kDa, which also suppressed P. aeruginosa autolysis and prevented killing by clinically relevant antibiotics through promoting small-colony variant (SCV) formation. The commensal interaction was abolished with S. aureus strains mutated in the agr quorum sensing system or in the SarA transcriptional virulence regulator, as well as with strains lacking the proteolytic subunit, ClpP, of the Clp protease. Our results show that during evolution of a dominant cystic fibrosis lineage of P. aeruginosa, a commensal interaction potential with S. aureus has developed. (Copyright © 2014, American Society for Microbiology. All Rights Reserved.)
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المشرفين على المادة:	0 (Anti-Bacterial Agents) 0 (Bacterial Proteins) 0 (Gentamicins) 5E8K9I0O4U (Ciprofloxacin)
تواريخ الأحداث:	Date Created: 20140904 Date Completed: 20150708 Latest Revision: 20211021
رمز التحديث:	20240628
مُعرف محوري في PubMed:	PMC4248816
DOI:	10.1128/JB.02006-14
PMID:	25182495
قاعدة البيانات:	MEDLINE

الوصف
تدمد:	1098-5530
DOI:	10.1128/JB.02006-14