دورية أكاديمية
HIV screening in blood donors: rapid diagnostic test versus enhanced ELISA.
| العنوان: | HIV screening in blood donors: rapid diagnostic test versus enhanced ELISA. |
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| المؤلفون: | Orkuma JA, Egesie JO, Banwat EB, Ejele AO, Orkuma JH, Bako IA |
| المصدر: | Nigerian journal of medicine : journal of the National Association of Resident Doctors of Nigeria [Niger J Med] 2014 Jul-Sep; Vol. 23 (3), pp. 192-200. |
| نوع المنشور: | Comparative Study; Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: The Association Country of Publication: Nigeria NLM ID: 100888321 Publication Model: Print Cited Medium: Print ISSN: 1115-2613 (Print) Linking ISSN: 11152613 NLM ISO Abbreviation: Niger J Med Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Lagos, Nigeria] : The Association, |
| مواضيع طبية MeSH: | Blood Donors*, AIDS Serodiagnosis/*methods , Enzyme-Linked Immunosorbent Assay/*methods, Adolescent ; Adult ; Cross-Sectional Studies ; Female ; Humans ; Male ; Middle Aged ; Nigeria ; Predictive Value of Tests ; Sensitivity and Specificity |
| مستخلص: | Objective: To compare the laboratory performance of a HIV antibody Rapid Diagnostic Test (RDT) [Determine HIV-1/2] with an Enhanced ELISA [GENSCREEN PLUS HIV Ag-Ab] used for HIV screening among blood donors in a hospital-based transfusion centre in North Central Nigeria. Methods: The serum of four hundred and forty (440) blood donors of both sexes aged between 18 and 55 years, who fulfilled the conditions to donate blood, were serially tested for HIV using both methods. Result: DetermineTM HIV-1/2 detected 16 (3.6%) while GENSCREEN PLUS HIV Ag-Ab ELISA detected 41 (9.3%) blood donors who were HIV sero-positive. The DetermineTM HIV-1/2 missed 25 (61%) blood donors who were positive with the GENSCREENPLUS HIV Ag-Ab ELISA. The calculated sensitivity and specificity of Determine HIV-1/2 were 39.0% and 100% respectively while the positive predictive value and negative predictive values of Determine HIV-1/2 were 100% and 94.1% respectively. Conclusion: The HIV Antibody RDT (Determine HIV-1/2) has a very low sensitivity and therefore, inadequate in preventing transfusion transmissible HIV (TT-HIV) in a hospital-based blood transfusion service. The National Blood Transfusion Service (NBTS) in Nigeria should be equipped and supported to provide blood products screened with Enhanced ELISA as well as to embark on regular evaluation of all HIV screening assays used in the country in order to prevent counterfeit and sub standard assays and invariably reduce the risk of TT-HIV infection in the country. |
| تواريخ الأحداث: | Date Created: 20140905 Date Completed: 20140925 Latest Revision: 20220410 |
| رمز التحديث: | 20231215 |
| PMID: | 25185375 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1115-2613 |
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