دورية أكاديمية
أعرض في EDS

The olfactory transcriptomes of mice.

التفاصيل البيبلوغرافية
العنوان: The olfactory transcriptomes of mice.
المؤلفون: Ibarra-Soria X; Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom., Levitin MO; Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom., Saraiva LR; Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom; European Molecular Biology Laboratory - European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom., Logan DW; Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom.
المصدر: PLoS genetics [PLoS Genet] 2014 Sep 04; Vol. 10 (9), pp. e1004593. Date of Electronic Publication: 2014 Sep 04 (Print Publication: 2014).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101239074 Publication Model: eCollection Cited Medium: Internet ISSN: 1553-7404 (Electronic) Linking ISSN: 15537390 NLM ISO Abbreviation: PLoS Genet Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science, c2005-
مواضيع طبية MeSH: Olfactory Mucosa/*pathology , Olfactory Perception/*genetics , Receptors, Odorant/*genetics , Smell/*genetics , Transcriptome/*genetics, Animals ; Female ; Gene Expression/genetics ; Genome/genetics ; High-Throughput Nucleotide Sequencing/methods ; Male ; Mice ; Mice, Inbred C57BL ; Pseudogenes/genetics ; Vomeronasal Organ/physiology
مستخلص: The olfactory (OR) and vomeronasal receptor (VR) repertoires are collectively encoded by 1700 genes and pseudogenes in the mouse genome. Most OR and VR genes were identified by comparative genomic techniques and therefore, in many of those cases, only their protein coding sequences are defined. Some also lack experimental support, due in part to the similarity between them and their monogenic, cell-specific expression in olfactory tissues. Here we use deep RNA sequencing, expression microarray and quantitative RT-PCR in both the vomeronasal organ and whole olfactory mucosa to quantify their full transcriptomes in multiple male and female mice. We find evidence of expression for all VR, and almost all OR genes that are annotated as functional in the reference genome, and use the data to generate over 1100 new, multi-exonic, significantly extended receptor gene annotations. We find that OR and VR genes are neither equally nor randomly expressed, but have reproducible distributions of abundance in both tissues. The olfactory transcriptomes are only minimally different between males and females, suggesting altered gene expression at the periphery is unlikely to underpin the striking sexual dimorphism in olfactory-mediated behavior. Finally, we present evidence that hundreds of novel, putatively protein-coding genes are expressed in these highly specialized olfactory tissues, and carry out a proof-of-principle validation. Taken together, these data provide a comprehensive, quantitative catalog of the genes that mediate olfactory perception and pheromone-evoked behavior at the periphery.
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معلومات مُعتمدة: United Kingdom Wellcome Trust; 098051 United Kingdom WT_ Wellcome Trust
المشرفين على المادة: 0 (Receptors, Odorant)
تواريخ الأحداث: Date Created: 20140905 Date Completed: 20151106 Latest Revision: 20211021
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC4154679
DOI: 10.1371/journal.pgen.1004593
PMID: 25187969
قاعدة البيانات: MEDLINE
الوصف
تدمد:1553-7404
DOI:10.1371/journal.pgen.1004593