دورية أكاديمية
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Evaluation of the reporting level to detect triamcinolone acetonide misuse in sports.

التفاصيل البيبلوغرافية
العنوان: Evaluation of the reporting level to detect triamcinolone acetonide misuse in sports.
المؤلفون: Matabosch X; Bioanalysis Research Group, IMIM (Institut Hospital del Mar d'Investigacions Mèdiques), Doctor Aiguader 88, 08003 Barcelona, Spain., Pozo OJ; Bioanalysis Research Group, IMIM (Institut Hospital del Mar d'Investigacions Mèdiques), Doctor Aiguader 88, 08003 Barcelona, Spain., Pérez-Mañá C; Human Pharmacology and Neurosciences Research Group, IMIM, Doctor Aiguader 88, 08003 Barcelona, Spain; Department of Pharmacology, Therapeutics and Toxicology, Universitat Autònoma de Barcelona, Bellaterra (Cerdanyola del Vallès), 08193 Barcelona, Spain., Papaseit E; Human Pharmacology and Neurosciences Research Group, IMIM, Doctor Aiguader 88, 08003 Barcelona, Spain; Department of Pharmacology, Therapeutics and Toxicology, Universitat Autònoma de Barcelona, Bellaterra (Cerdanyola del Vallès), 08193 Barcelona, Spain., Marcos J; Bioanalysis Research Group, IMIM (Institut Hospital del Mar d'Investigacions Mèdiques), Doctor Aiguader 88, 08003 Barcelona, Spain; Department of Experimental and Health Sciences, Universitat Pompeu Fabra, Doctor Aiguader 88, 08003 Barcelona, Spain., Segura J; Bioanalysis Research Group, IMIM (Institut Hospital del Mar d'Investigacions Mèdiques), Doctor Aiguader 88, 08003 Barcelona, Spain; Department of Experimental and Health Sciences, Universitat Pompeu Fabra, Doctor Aiguader 88, 08003 Barcelona, Spain., Ventura R; Bioanalysis Research Group, IMIM (Institut Hospital del Mar d'Investigacions Mèdiques), Doctor Aiguader 88, 08003 Barcelona, Spain; Department of Experimental and Health Sciences, Universitat Pompeu Fabra, Doctor Aiguader 88, 08003 Barcelona, Spain. Electronic address: rventura@imim.es.
المصدر: The Journal of steroid biochemistry and molecular biology [J Steroid Biochem Mol Biol] 2015 Jan; Vol. 145, pp. 94-102. Date of Electronic Publication: 2014 Sep 24.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Pergamon Country of Publication: England NLM ID: 9015483 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1220 (Electronic) Linking ISSN: 09600760 NLM ISO Abbreviation: J Steroid Biochem Mol Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Oxford ; New York : Pergamon, c1990-
مواضيع طبية MeSH: Doping in Sports* , Urinalysis*, Triamcinolone Acetonide/*urine, Administration, Intranasal ; Administration, Topical ; Adult ; Calibration ; Chromatography, Liquid ; False Positive Reactions ; Healthy Volunteers ; Humans ; Injections, Intramuscular ; Male ; Middle Aged ; Quality Control ; Reproducibility of Results ; Sensitivity and Specificity ; Sports ; Tandem Mass Spectrometry ; Young Adult
مستخلص: Triamcinolone acetonide (TA) is prohibited in sport competitions using systemic administrations (e.g., intramuscular, IM), and it is allowed by other routes (e.g., intranasal, IN, or topical, TOP). A reporting level of 30 ng/mL is used to discriminate between forbidden and allowed administrations. We examined urinary profiles of TA metabolites after TOP, IN and IM administrations to evaluate the suitability of the current reporting level and to define the best criteria to discriminate between these administrations. TA was administered to healthy volunteers by different routes: a single IM dose (n=2), IN doses for three days (n=6), and TOP doses for five days followed by a single IM dose (n=8). Urine samples were collected at different time intervals and they were analyzed by liquid chromatography-tandem mass spectrometry to measure TA and eight metabolites. After TOP and IN administrations, concentrations of the metabolites were significantly lower (p<0.05) than after IM administrations. Concentrations of TA after IM administration were lower than 30 ng/mL for all volunteers (range 0.7-29.7 ng/mL), and they were lower than 5 ng/mL after multiple IN or TOP doses (0.1-3.6 ng/mL and 0-1.7 ng/mL, respectively). For 6β-hydroxy-TA, the main TA metabolite, greater concentrations were obtained: 10.7-469.1 ng/mL, 2.2-90.6 ng/mL and 0-57.2 ng/mL after IM, IN and TOP administrations, respectively. These results suggest that the current reporting level is not suitable to detect forbidden IM administration of TA. A lower concentration of the parent drug or the use of specific metabolites could discriminate IM from TOP or IN administrations.
(Copyright © 2014 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Administration routes; Doping; LC–MS/MS; Metabolites; Triamcinolone acetonide
المشرفين على المادة: F446C597KA (Triamcinolone Acetonide)
تواريخ الأحداث: Date Created: 20140928 Date Completed: 20150223 Latest Revision: 20141216
رمز التحديث: 20240628
DOI: 10.1016/j.jsbmb.2014.09.018
PMID: 25260353
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-1220
DOI:10.1016/j.jsbmb.2014.09.018