دورية أكاديمية
Photonic detection and characterization of DNA using sapphire microspheres.
| العنوان: | Photonic detection and characterization of DNA using sapphire microspheres. |
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| المؤلفون: | Murib MS; Hasselt University, Instituut voor Materiaalonderzoek, Wetenschapspark 1, B-3590 Diepenbeek, Belgium., Yeap WS; Hasselt University, Instituut voor Materiaalonderzoek, Wetenschapspark 1, B-3590 Diepenbeek, Belgium., Martens D; Ghent University-Information Technology, Department of Information Technology, Photonics Research Group, Sint-Pietersnieuwstraat 41, B-9000 Gent, Belgium., Bienstman P; Ghent University-Information Technology, Department of Information Technology, Photonics Research Group, Sint-Pietersnieuwstraat 41, B-9000 Gent, Belgium., De Ceuninck W; Hasselt University, Instituut voor Materiaalonderzoek, Wetenschapspark 1, B-3590 Diepenbeek, BelgiumcInteruniversitair Microelectronica Centrum vereniging zonder winstoogmerk, Division Instituut voor Materiaalonderzoek in de Micro-Elektronica, Wetenschaps., van Grinsven B; Hasselt University, Instituut voor Materiaalonderzoek, Wetenschapspark 1, B-3590 Diepenbeek, BelgiumcInteruniversitair Microelectronica Centrum vereniging zonder winstoogmerk, Division Instituut voor Materiaalonderzoek in de Micro-Elektronica, Wetenschaps., Schöning MJ; Aachen University of Applied Sciences, Institute of Nano- and Biotechnologies, Heinrich-Mußmann-Straße 1, D-52428 Jülich, Germany., Michiels L; Hasselt University, BIOMED, Agoralaan Building C, B-3590 Diepenbeek, Belgium., Haenen K; Hasselt University, Instituut voor Materiaalonderzoek, Wetenschapspark 1, B-3590 Diepenbeek, BelgiumcInteruniversitair Microelectronica Centrum vereniging zonder winstoogmerk, Division Instituut voor Materiaalonderzoek in de Micro-Elektronica, Wetenschaps., Ameloot M; Hasselt University, BIOMED, Agoralaan Building C, B-3590 Diepenbeek, Belgium., Serpengüzel A; Koç University, Department of Physics, Microphotonics Research Laboratory, Rumelifeneri Yolu, Sariyer, Istanbul 34450, Turkey., Wagner P; Hasselt University, Instituut voor Materiaalonderzoek, Wetenschapspark 1, B-3590 Diepenbeek, BelgiumcInteruniversitair Microelectronica Centrum vereniging zonder winstoogmerk, Division Instituut voor Materiaalonderzoek in de Micro-Elektronica, Wetenschaps. |
| المصدر: | Journal of biomedical optics [J Biomed Opt] 2014 Sep; Vol. 19 (9), pp. 97006. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Published by SPIE--the International Society for Optical Engineering in cooperation with International Biomedical Optics Society Country of Publication: United States NLM ID: 9605853 Publication Model: Print Cited Medium: Internet ISSN: 1560-2281 (Electronic) Linking ISSN: 10833668 NLM ISO Abbreviation: J Biomed Opt Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Bellingham, WA : Published by SPIE--the International Society for Optical Engineering in cooperation with International Biomedical Optics Society, c1996- |
| مواضيع طبية MeSH: | Microspheres* , Scattering, Radiation*, Aluminum Oxide/*chemistry , DNA/*chemistry , Optics and Photonics/*methods, DNA/analysis ; Light ; Microscopy, Confocal |
| مستخلص: | A microcavity-based deoxyribonucleic acid (DNA) optical biosensor is demonstrated for the first time using synthetic sapphire for the optical cavity. Transmitted and elastic scattering intensity at 1510 nm are analyzed from a sapphire microsphere (radius 500 µm, refractive index 1.77) on an optical fiber half coupler. The 0.43 nm angular mode spacing of the resonances correlates well with the optical size of the sapphire sphere. Probe DNA consisting of a 36-mer fragment was covalently immobilized on a sapphire microsphere and hybridized with a 29-mer target DNA. Whispering gallery modes (WGMs) were monitored before the sapphire was functionalized with DNA and after it was functionalized with single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). The shift in WGMs from the surface modification with DNA was measured and correlated well with the estimated thickness of the add-on DNA layer. It is shown that ssDNA is more uniformly oriented on the sapphire surface than dsDNA. In addition, it is shown that functionalization of the sapphire spherical surface with DNA does not affect the quality factor (Q . ≈ 04) of the sapphire microspheres. The use of sapphire is especially interesting because this material is chemically resilient, biocompatible, and widely used for medical implants. |
| المشرفين على المادة: | 9007-49-2 (DNA) LMI26O6933 (Aluminum Oxide) |
| تواريخ الأحداث: | Date Created: 20140928 Date Completed: 20150514 Latest Revision: 20190930 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1117/1.JBO.19.9.097006 |
| PMID: | 25260868 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1560-2281 |
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| DOI: | 10.1117/1.JBO.19.9.097006 |